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Generation of a functional, soluble tapasin protein from an alternatively spliced mRNA

Record type: Article

The loading of newly synthesised MHC class I molecules (MHCI) with peptides requires the involvement of several endoplasmic reticulum (ER)-resident cofactors including calnexin, calreticulin, transporter associated with antigen processing, ERp57 and tapasin. In the absence of tapasin, MHC I complexes are loaded with suboptimal peptides and their recognition by cytotoxic T cells raised to high-affinity, immunodominant peptide epitopes is impaired. Here, we describe the cloning and functional assessment of an alternative spliced form of tapasin. From the EST database, we obtained a partially spliced tapasin cDNA that retained introns 4-6. When transfected into the tapasin-deficient cell line 0.220, the cDNA produced an alternatively spliced tapasin transcript that contained intron 5 (74 bp). This introduced a new stop codon that terminated translation immediately before the putative transmembrane domain and led to a tapasin molecule containing the lumenal domain plus 8 extra novel amino acids at its C-terminus. This molecule promoted peptide loading of HLA-B5 in 0.220 cell line, and restored normal HLA-B5 surface expression. However, the peptides loaded onto HLA-B5 were suboptimal compared to those loaded onto HLA-B5 in the presence of wild-type tapasin.

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Citation

Gao, B., Williams, A., Sewell, A. and Elliott, T. (2004) Generation of a functional, soluble tapasin protein from an alternatively spliced mRNA Genes and Immunity, 5, (2), pp. 101-108. (doi:10.1038/sj.gene.6364043). (PMID:14668790).

More information

Published date: March 2004
Keywords: tapasin, antigen processing, MHC class I assembly
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 384929
URI: http://eprints.soton.ac.uk/id/eprint/384929
ISSN: 1466-4879
PURE UUID: 1c1899fd-0bb0-4185-b803-5b8de07623e5
ORCID for T. Elliott: ORCID iD orcid.org/0000-0003-1097-0222

Catalogue record

Date deposited: 21 Jan 2016 11:32
Last modified: 17 Jul 2017 20:01

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Contributors

Author: B. Gao
Author: A. Williams
Author: A. Sewell
Author: T. Elliott ORCID iD

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