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Selective and compartmentalized myelin expression of HspB5

Selective and compartmentalized myelin expression of HspB5
Selective and compartmentalized myelin expression of HspB5
In the present study, we reveal myelin specific expression and targeting of mRNA and biochemical pools of HspB5 in the mouse CNS. Our observations are based on in-situ hybridisation, electron microscopy and co-localisation with 2’,3’-Cyclin-Nucleotide 3’-Phosphodiesterase (CNPase), reinforcing this myelin selective expression. HspB5 mRNA might be targeted to these structures based on its presence in discrete clusters resembling RNA granules and the presence of a putative RNA transport signal. Further, sub-cellular fractionation of myelin membranes reveals a distinct sub-compartment specific association and detergent solubility of HspB5. This is akin to other abundant myelin proteins and is consistent with HspB5’s association with cytoskeletal/membrane assemblies. Oligodendrocytes have a pivotal role in supporting axonal function via generating and segregating the ensheathing myelin. This specialisation places extreme structural and metabolic demands on this glial cell type. Our observations place HspB5 in oligodendrocytes which may require selective and specific chaperone capabilities to maintain normal function and neuronal support.
myelin, small heat-shock protein, alphaB-crystallin, oligodendrocyte, cns, chaperone
0306-4522
130-142
Quraishe, Shmma
cfc3aed4-f120-41aa-9127-0fc26c657ad2
Wyttenbach, Andreas
05019897-52b1-4bb6-b259-5d51abae7540
Matinyarare, Nyasha
6a316e36-03ad-40cc-9baf-2790e18bd15a
Perry, Victor
8f29d36a-8e1f-4082-8700-09483bbaeae4
Fern, Robert
62aed936-dc63-4135-8cea-9fdd3ca8e788
O'Connor, Vincent
8021b06c-01a0-4925-9dde-a61c8fe278ca
Quraishe, Shmma
cfc3aed4-f120-41aa-9127-0fc26c657ad2
Wyttenbach, Andreas
05019897-52b1-4bb6-b259-5d51abae7540
Matinyarare, Nyasha
6a316e36-03ad-40cc-9baf-2790e18bd15a
Perry, Victor
8f29d36a-8e1f-4082-8700-09483bbaeae4
Fern, Robert
62aed936-dc63-4135-8cea-9fdd3ca8e788
O'Connor, Vincent
8021b06c-01a0-4925-9dde-a61c8fe278ca

Quraishe, Shmma, Wyttenbach, Andreas, Matinyarare, Nyasha, Perry, Victor, Fern, Robert and O'Connor, Vincent (2016) Selective and compartmentalized myelin expression of HspB5. Neuroscience, 316, 130-142. (doi:10.1016/j.neuroscience.2015.12.035). (PMID:26718604)

Record type: Article

Abstract

In the present study, we reveal myelin specific expression and targeting of mRNA and biochemical pools of HspB5 in the mouse CNS. Our observations are based on in-situ hybridisation, electron microscopy and co-localisation with 2’,3’-Cyclin-Nucleotide 3’-Phosphodiesterase (CNPase), reinforcing this myelin selective expression. HspB5 mRNA might be targeted to these structures based on its presence in discrete clusters resembling RNA granules and the presence of a putative RNA transport signal. Further, sub-cellular fractionation of myelin membranes reveals a distinct sub-compartment specific association and detergent solubility of HspB5. This is akin to other abundant myelin proteins and is consistent with HspB5’s association with cytoskeletal/membrane assemblies. Oligodendrocytes have a pivotal role in supporting axonal function via generating and segregating the ensheathing myelin. This specialisation places extreme structural and metabolic demands on this glial cell type. Our observations place HspB5 in oligodendrocytes which may require selective and specific chaperone capabilities to maintain normal function and neuronal support.

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Accepted/In Press date: 17 December 2015
e-pub ahead of print date: 21 December 2015
Published date: 1 March 2016
Keywords: myelin, small heat-shock protein, alphaB-crystallin, oligodendrocyte, cns, chaperone
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 385295
URI: http://eprints.soton.ac.uk/id/eprint/385295
ISSN: 0306-4522
PURE UUID: c165ae36-1c73-451f-8ecc-53ed6595280d

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Date deposited: 14 Mar 2016 13:45
Last modified: 02 Dec 2019 20:27

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