Simple and rapid sample preparation system for the molecular detection of antibiotic resistant bacteria in human urine
Simple and rapid sample preparation system for the molecular detection of antibiotic resistant bacteria in human urine
Antibiotic resistance in urinary tract infections (UTIs) can cause significant complications without quick detection and appropriate treatment. . We describe a new approach to capture, concentrate and prepare amplification-ready DNA from antibiotic resistant bacteria in human urine samples. Klebsiella pneumoniae NCTC13443 (blaCTX-M-15 positive) spiked into filtered human urine was used as a model system. Bacteria were captured using anion exchange diaethylaminoethyl (DEAE) magnetic microparticles and concentrated 200-fold within ~3.5 minutes using a custom, valve-less microfluidic chip. Eight samples were processed in parallel, and DNA, was released using heat lysis from an integrated resistive heater. The crude cell lysate was used for real time Recombinase Polymerase Amplification (RPA) of the blaCTX-M-15 gene. The end to end processing time was approximately 15 minutes with a limit of detection of 1000 bacteria in 1 mL urine
1-30
Valiadi, Martha
e245e9c5-3e97-4896-b910-c4178c5fb3c9
Kalsi, Sumit
65cb3d4c-c28c-490a-85a3-7a8fb96ecfee
Jones, Isaac
f550a9db-f6fd-43cb-825c-bbbaee87cf88
Turner, Carrie
92e6c17b-41e4-4867-9812-677e4cb88996
Sutton, Mark
3ffaeca5-caf0-4d77-a573-fa4763387149
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Valiadi, Martha
e245e9c5-3e97-4896-b910-c4178c5fb3c9
Kalsi, Sumit
65cb3d4c-c28c-490a-85a3-7a8fb96ecfee
Jones, Isaac
f550a9db-f6fd-43cb-825c-bbbaee87cf88
Turner, Carrie
92e6c17b-41e4-4867-9812-677e4cb88996
Sutton, Mark
3ffaeca5-caf0-4d77-a573-fa4763387149
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Valiadi, Martha, Kalsi, Sumit, Jones, Isaac, Turner, Carrie, Sutton, Mark and Morgan, Hywel
(2016)
Simple and rapid sample preparation system for the molecular detection of antibiotic resistant bacteria in human urine.
Biomedical Microdevices, .
(In Press)
Abstract
Antibiotic resistance in urinary tract infections (UTIs) can cause significant complications without quick detection and appropriate treatment. . We describe a new approach to capture, concentrate and prepare amplification-ready DNA from antibiotic resistant bacteria in human urine samples. Klebsiella pneumoniae NCTC13443 (blaCTX-M-15 positive) spiked into filtered human urine was used as a model system. Bacteria were captured using anion exchange diaethylaminoethyl (DEAE) magnetic microparticles and concentrated 200-fold within ~3.5 minutes using a custom, valve-less microfluidic chip. Eight samples were processed in parallel, and DNA, was released using heat lysis from an integrated resistive heater. The crude cell lysate was used for real time Recombinase Polymerase Amplification (RPA) of the blaCTX-M-15 gene. The end to end processing time was approximately 15 minutes with a limit of detection of 1000 bacteria in 1 mL urine
PDF
Valiadi et al submission Biomed Microdev.pdf
- Accepted Manuscript
More information
Accepted/In Press date: 5 January 2016
Organisations:
Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 385553
URI: http://eprints.soton.ac.uk/id/eprint/385553
ISSN: 1387-2176
PURE UUID: 325aeea2-7701-4c3a-87ce-a93b9700850a
Catalogue record
Date deposited: 20 Jan 2016 10:27
Last modified: 06 Jun 2018 12:45
Export record
Contributors
Author:
Martha Valiadi
Author:
Sumit Kalsi
Author:
Isaac Jones
Author:
Carrie Turner
Author:
Mark Sutton
Author:
Hywel Morgan
University divisions
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics