A polysaccharide from Andrographis paniculata induces mitochondrial-mediated apoptosis in human hepatoma cell line (HepG2)
A polysaccharide from Andrographis paniculata induces mitochondrial-mediated apoptosis in human hepatoma cell line (HepG2)
In the present study, we investigated the effects and action mechanisms of a purified polysaccharide (APWP) from Andrographis paniculata, on human hepatocellular carcinoma (HCC) HepG2 cells. The results showed that APWP was able to suppress the proliferation of HepG2 cells via inducing apoptosis. Western blot analysis revealed that dose-dependent increase in proapoptotic Bax protein and no change in antiapoptotic Bcl-2 protein in APWP-treated cells. Furthermore, exposure of tumor cells to APWP resulted in a loss of mitochondrial membrane potential (MMP) and the release of cytochrome c from the mitochondria to the cytosol. Besides, caspase-9 and caspase-3 were activated while caspase-8 was not affected in HepG2 cells followed by APWP treatment. All these results point clearly to the involvement of mitochondria-mediated signaling pathway in APWP-induced apoptosis and strongly suggest that APWP seems to be safe and effective in the prevention and treatment of HCC.
andrographis paniculata, polysaccharides, hepatocellular carcinoma, apoptosis, mitochondria
5179-5186
Zou, Yanmei
6783820d-2ff0-4900-9ade-34fe1180f56f
Xiong, Hua
f80e5b10-9793-40ae-8143-1762577028f4
Xiong, Huihua
c835b64f-d8a2-4a72-8c53-b730db6ded56
Lu, Tao
1658fb42-f32a-416d-86b5-3ab28dda8338
Zhu, Feng
a9499a5c-c42b-4fc0-91dd-0a7f830e6ce0
Luo, Zhiyong
c770d311-3ca9-41de-8ce9-f560a0ed79b1
Yuan, Xianglin
54dfb6d3-78bf-48da-936d-ded37bfcf238
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
July 2015
Zou, Yanmei
6783820d-2ff0-4900-9ade-34fe1180f56f
Xiong, Hua
f80e5b10-9793-40ae-8143-1762577028f4
Xiong, Huihua
c835b64f-d8a2-4a72-8c53-b730db6ded56
Lu, Tao
1658fb42-f32a-416d-86b5-3ab28dda8338
Zhu, Feng
a9499a5c-c42b-4fc0-91dd-0a7f830e6ce0
Luo, Zhiyong
c770d311-3ca9-41de-8ce9-f560a0ed79b1
Yuan, Xianglin
54dfb6d3-78bf-48da-936d-ded37bfcf238
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Zou, Yanmei, Xiong, Hua, Xiong, Huihua, Lu, Tao, Zhu, Feng, Luo, Zhiyong, Yuan, Xianglin and Wang, Yihua
(2015)
A polysaccharide from Andrographis paniculata induces mitochondrial-mediated apoptosis in human hepatoma cell line (HepG2).
Tumor Biology, 36 (7), .
(doi:10.1007/s13277-015-3172-1).
(PMID:25652470)
Abstract
In the present study, we investigated the effects and action mechanisms of a purified polysaccharide (APWP) from Andrographis paniculata, on human hepatocellular carcinoma (HCC) HepG2 cells. The results showed that APWP was able to suppress the proliferation of HepG2 cells via inducing apoptosis. Western blot analysis revealed that dose-dependent increase in proapoptotic Bax protein and no change in antiapoptotic Bcl-2 protein in APWP-treated cells. Furthermore, exposure of tumor cells to APWP resulted in a loss of mitochondrial membrane potential (MMP) and the release of cytochrome c from the mitochondria to the cytosol. Besides, caspase-9 and caspase-3 were activated while caspase-8 was not affected in HepG2 cells followed by APWP treatment. All these results point clearly to the involvement of mitochondria-mediated signaling pathway in APWP-induced apoptosis and strongly suggest that APWP seems to be safe and effective in the prevention and treatment of HCC.
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Accepted/In Press date: 26 January 2015
e-pub ahead of print date: 5 February 2015
Published date: July 2015
Keywords:
andrographis paniculata, polysaccharides, hepatocellular carcinoma, apoptosis, mitochondria
Organisations:
Centre for Biological Sciences
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Local EPrints ID: 385896
URI: http://eprints.soton.ac.uk/id/eprint/385896
ISSN: 1010-4283
PURE UUID: eef0adfc-e102-447f-a2f0-77805b97bfc9
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Date deposited: 25 Jan 2016 17:02
Last modified: 15 Mar 2024 03:52
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Author:
Yanmei Zou
Author:
Hua Xiong
Author:
Huihua Xiong
Author:
Tao Lu
Author:
Feng Zhu
Author:
Zhiyong Luo
Author:
Xianglin Yuan
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