Tissue microarray analysis of human FRAT1 expression and its correlation with the subcellular localisation of ?-catenin in ovarian tumours
Tissue microarray analysis of human FRAT1 expression and its correlation with the subcellular localisation of ?-catenin in ovarian tumours
The mechanisms involved in the pathogenesis of ovarian cancer are poorly understood, but evidence suggests that aberrant activation of Wnt/?-catenin signalling pathway plays a significant role in this malignancy. However, the molecular defects that contribute to the activation of this pathway have not been elucidated. Frequently rearranged in advanced T-cell lymphomas-1 (FRAT1) is a candidate for the regulation of cytoplasmic ?-catenin. In this study, we developed in situ hybridisation probes to evaluate the presence of FRAT1 and used an anti-?-catenin antibody to evaluate by immunohistochemistry the expression levels and subcellular localisation of ?-catenin in ovarian cancer tissue microarrays. Expression of FRAT1 was found in some human normal tissues and 47% of ovarian adenocarcinomas. A total of 46% of ovarian serous adenocarcinomas were positive for FRAT1 expression. Accumulation of ?-catenin in the nucleus and/or cytoplasm was observed in 55% ovarian adenocarcinomas and in 59% of serous adenocarcinomas. A significant association was observed in ovarian serous adenocarcinomas between FRAT1 and ?-catenin expression (P<0.01). These findings support that Wnt/?-catenin signalling may be aberrantly activated through FRAT1 overexpression in ovarian serous adenocarcinomas. The mechanism behind the overexpression of FRAT1 in ovarian serous adenocarcinomas and its significance is yet to be investigated.
FRAT1, ?-catenin, wnt pathway, ovarian cancer, tissue microarray
686-691
Wang, Y.
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Hewitt, S.M.
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Liu, S.
3554b4ea-f13f-4ea0-af48-e7559d076cb5
Zhou, X.
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Zhu, H.
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Zhou, C.
b01ced75-65f3-44b3-88ba-00fe9d5efb44
Zhang, G.
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Quan, L.
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Bai, J.
823a05e0-144e-4dad-97f3-f8d942141e6c
Xu, N.
ea97ad95-80e3-4c01-a36c-9826718726b9
7 March 2006
Wang, Y.
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Hewitt, S.M.
20824f4c-003d-42d0-bc78-e271957f9e67
Liu, S.
3554b4ea-f13f-4ea0-af48-e7559d076cb5
Zhou, X.
bee0e911-42d5-4854-8520-cf87faecb3a9
Zhu, H.
56de08ce-886f-4d04-bbc5-ee175ce014ae
Zhou, C.
b01ced75-65f3-44b3-88ba-00fe9d5efb44
Zhang, G.
ed8f311f-ae14-4489-970f-c6ba8c36fcd2
Quan, L.
bbf3ef89-8345-4715-9201-720998f8a248
Bai, J.
823a05e0-144e-4dad-97f3-f8d942141e6c
Xu, N.
ea97ad95-80e3-4c01-a36c-9826718726b9
Wang, Y., Hewitt, S.M., Liu, S., Zhou, X., Zhu, H., Zhou, C., Zhang, G., Quan, L., Bai, J. and Xu, N.
(2006)
Tissue microarray analysis of human FRAT1 expression and its correlation with the subcellular localisation of ?-catenin in ovarian tumours.
British Journal of Cancer, 94 (5), .
(doi:10.1038/sj.bjc.6602988).
(PMID:16479254)
Abstract
The mechanisms involved in the pathogenesis of ovarian cancer are poorly understood, but evidence suggests that aberrant activation of Wnt/?-catenin signalling pathway plays a significant role in this malignancy. However, the molecular defects that contribute to the activation of this pathway have not been elucidated. Frequently rearranged in advanced T-cell lymphomas-1 (FRAT1) is a candidate for the regulation of cytoplasmic ?-catenin. In this study, we developed in situ hybridisation probes to evaluate the presence of FRAT1 and used an anti-?-catenin antibody to evaluate by immunohistochemistry the expression levels and subcellular localisation of ?-catenin in ovarian cancer tissue microarrays. Expression of FRAT1 was found in some human normal tissues and 47% of ovarian adenocarcinomas. A total of 46% of ovarian serous adenocarcinomas were positive for FRAT1 expression. Accumulation of ?-catenin in the nucleus and/or cytoplasm was observed in 55% ovarian adenocarcinomas and in 59% of serous adenocarcinomas. A significant association was observed in ovarian serous adenocarcinomas between FRAT1 and ?-catenin expression (P<0.01). These findings support that Wnt/?-catenin signalling may be aberrantly activated through FRAT1 overexpression in ovarian serous adenocarcinomas. The mechanism behind the overexpression of FRAT1 in ovarian serous adenocarcinomas and its significance is yet to be investigated.
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Accepted/In Press date: 17 January 2006
e-pub ahead of print date: 14 February 2006
Published date: 7 March 2006
Keywords:
FRAT1, ?-catenin, wnt pathway, ovarian cancer, tissue microarray
Organisations:
Centre for Biological Sciences
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Local EPrints ID: 385914
URI: http://eprints.soton.ac.uk/id/eprint/385914
ISSN: 0007-0920
PURE UUID: adb539c2-7cc0-47c7-a60b-ff8068c8485c
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Date deposited: 26 Jan 2016 11:12
Last modified: 15 Mar 2024 03:52
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Author:
S.M. Hewitt
Author:
S. Liu
Author:
X. Zhou
Author:
H. Zhu
Author:
C. Zhou
Author:
G. Zhang
Author:
L. Quan
Author:
J. Bai
Author:
N. Xu
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