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Overexpression of human pituitary tumor transforming gene (hPTTG), is regulated by beta-catenin /TCF pathway in human esophageal squamous cell carcinoma

Overexpression of human pituitary tumor transforming gene (hPTTG), is regulated by beta-catenin /TCF pathway in human esophageal squamous cell carcinoma
Overexpression of human pituitary tumor transforming gene (hPTTG), is regulated by beta-catenin /TCF pathway in human esophageal squamous cell carcinoma
Overexpression of human pituitary tumor transforming gene (PTTG) is wildly detected in many tumors, including esophageal cancer. Besides overexpression of PTTG in esophageal squamous cell carcinoma (ESCC) tissues and cells, we detected accumulation of cytoplasmic ?-catenin in ESCC. In our study, a putative TCF4-binding element (TBE) was identified in PTTG promoter region. The activity of PTTG promoter containing the TBE was activated by S37A?-catenin and inhibited by dominant-negative TCF. Furthermore, the activation by S37A?-catenin was mostly abrogated among PTTG promoter region without the TBE or with a mutant one. By using biotin-streptavidin pull-down assay, we also found that the TBE among PTTG promoter bound to TCF-4 protein. Moreover, levels of PTTG mRNA and protein were increased by S37A?-catenin. Finally, it is noticeable that we detected a correlation between ?-catenin localization and PTTG expression in 69 primary ESCC (p<0.01). In brief, our study shows that overexpression of PTTG in ESCC is likely due to the activation of ?-catenin/WNT signaling. As a target gene of ?-catenin/TCF pathway, PTTG may play an important role in tumorigenesis of human ESCC
hPTTG, overexpression, ?-catenin, target gene, ESCC
0020-7136
891-898
Zhou, Cuiqi
a79cddd5-12f5-41fd-af17-f553062f6d70
Liu, Shuang
f136b833-cd10-476d-80e2-8e8c69ee69bc
Zhou, Xiaobo
0deca180-bc8a-42d6-b6a6-237e04cae0e1
Xue, Liyan
d396faa2-726e-4672-aec4-7f56d9e186b0
Quan, Lanping
75ae7877-e353-41d9-ba4b-c3ad47e5a69c
Lu, Ning
8d60125f-c459-4a0e-8d87-c8e0123d40a5
Zhang, Guo
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Bai, Jinfeng
66033867-936e-4551-96db-61c624e804f1
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Liu, Zhihua
5b57dc8c-3339-4b29-a4aa-c44512cf4b41
Zhan, Qimin
c125182f-9a51-4062-93f0-9a30d98942f1
Zhu, Hongxia
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Xu, Ningzhi
7e2ddae3-ef56-4e9e-9ccb-4fddbcdf045f
Zhou, Cuiqi
a79cddd5-12f5-41fd-af17-f553062f6d70
Liu, Shuang
f136b833-cd10-476d-80e2-8e8c69ee69bc
Zhou, Xiaobo
0deca180-bc8a-42d6-b6a6-237e04cae0e1
Xue, Liyan
d396faa2-726e-4672-aec4-7f56d9e186b0
Quan, Lanping
75ae7877-e353-41d9-ba4b-c3ad47e5a69c
Lu, Ning
8d60125f-c459-4a0e-8d87-c8e0123d40a5
Zhang, Guo
7bce7bd5-04a3-48ac-8330-6f3521fab734
Bai, Jinfeng
66033867-936e-4551-96db-61c624e804f1
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Liu, Zhihua
5b57dc8c-3339-4b29-a4aa-c44512cf4b41
Zhan, Qimin
c125182f-9a51-4062-93f0-9a30d98942f1
Zhu, Hongxia
34c74461-aab8-4886-8ed4-f80438fa2083
Xu, Ningzhi
7e2ddae3-ef56-4e9e-9ccb-4fddbcdf045f

Zhou, Cuiqi, Liu, Shuang, Zhou, Xiaobo, Xue, Liyan, Quan, Lanping, Lu, Ning, Zhang, Guo, Bai, Jinfeng, Wang, Yihua, Liu, Zhihua, Zhan, Qimin, Zhu, Hongxia and Xu, Ningzhi (2005) Overexpression of human pituitary tumor transforming gene (hPTTG), is regulated by beta-catenin /TCF pathway in human esophageal squamous cell carcinoma. International Journal of Cancer, 113 (6), 891-898. (doi:10.1002/ijc.20642). (PMID:15514942)

Record type: Article

Abstract

Overexpression of human pituitary tumor transforming gene (PTTG) is wildly detected in many tumors, including esophageal cancer. Besides overexpression of PTTG in esophageal squamous cell carcinoma (ESCC) tissues and cells, we detected accumulation of cytoplasmic ?-catenin in ESCC. In our study, a putative TCF4-binding element (TBE) was identified in PTTG promoter region. The activity of PTTG promoter containing the TBE was activated by S37A?-catenin and inhibited by dominant-negative TCF. Furthermore, the activation by S37A?-catenin was mostly abrogated among PTTG promoter region without the TBE or with a mutant one. By using biotin-streptavidin pull-down assay, we also found that the TBE among PTTG promoter bound to TCF-4 protein. Moreover, levels of PTTG mRNA and protein were increased by S37A?-catenin. Finally, it is noticeable that we detected a correlation between ?-catenin localization and PTTG expression in 69 primary ESCC (p<0.01). In brief, our study shows that overexpression of PTTG in ESCC is likely due to the activation of ?-catenin/WNT signaling. As a target gene of ?-catenin/TCF pathway, PTTG may play an important role in tumorigenesis of human ESCC

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More information

Accepted/In Press date: 6 August 2004
e-pub ahead of print date: 28 October 2004
Published date: 1 March 2005
Keywords: hPTTG, overexpression, ?-catenin, target gene, ESCC
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 385916
URI: http://eprints.soton.ac.uk/id/eprint/385916
ISSN: 0020-7136
PURE UUID: 9787aa5b-ef9e-4a7f-bba4-5db494b875d1
ORCID for Yihua Wang: ORCID iD orcid.org/0000-0001-5561-0648

Catalogue record

Date deposited: 26 Jan 2016 11:19
Last modified: 01 Oct 2019 00:33

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Contributors

Author: Cuiqi Zhou
Author: Shuang Liu
Author: Xiaobo Zhou
Author: Liyan Xue
Author: Lanping Quan
Author: Ning Lu
Author: Guo Zhang
Author: Jinfeng Bai
Author: Yihua Wang ORCID iD
Author: Zhihua Liu
Author: Qimin Zhan
Author: Hongxia Zhu
Author: Ningzhi Xu

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