The University of Southampton
University of Southampton Institutional Repository

Accumulation of cytoplasmic beta-catenin correlates with reduced expression of E-cadherin, but not with phosphorylated Akt in esophageal squamous cell carcinoma: immunohistochemical study

Accumulation of cytoplasmic beta-catenin correlates with reduced expression of E-cadherin, but not with phosphorylated Akt in esophageal squamous cell carcinoma: immunohistochemical study
Accumulation of cytoplasmic beta-catenin correlates with reduced expression of E-cadherin, but not with phosphorylated Akt in esophageal squamous cell carcinoma: immunohistochemical study
Accumulation of ?-catenin in cytoplasm occurs frequently during the pathogenesis of esophageal squamous cell carcinoma (ESCC). The mechanism leading to this alteration, however, is largely unknown. In the present study, immunohistochemistry was performed for ?-catenin, E-cadherin and Ser473 phosphorylated Akt (P-Akt) in 44 tissue samples of ESCC and corresponding normal esophageal epithelium. Exon 3 of the ?-catenin gene was analyzed by using single-strand conformation polymorphism and direct sequencing. In addition to the reduced expression of E-cadherin and membranous ?-catenin observed in 65.9% and 68% of ESCC tested, respectively, cytoplasmic accumulation of ?-catenin was also detected in 68% (30/44) cases. However, only two cases were found to have the same ?-catenin gene mutation. The data showed that cytoplasmic accumulation of??-catenin was significantly associated ?with? reduced? expression? of? E-cadherin? (P < 0.05) and that of membranous ?-catenin (P < 0.05). Furthermore, cytoplasmic ?-catenin was correlated significantly with lymph node metastasis (P < 0.05). In contrast, although strong staining of P-Akt occurred in 14 of 44 cases (32%), there was no significant correlation between the positive staining of P-Akt and cytoplasmic ?-catenin. Taken together these results suggest that the lost membranous ?-catenin might translocate to cytoplasm depending on reduced expression of E-cadherin, while Akt seems unlikely to play a role in this process.
?-catenin, E-cadherin, esophagus, immunohistochemistry, P-Akt, squamous cell carcinoma
1320-5463
310-317
Zhang, Guo
7bce7bd5-04a3-48ac-8330-6f3521fab734
Zhou, Xiaobo
0deca180-bc8a-42d6-b6a6-237e04cae0e1
Xue, Liyan
d396faa2-726e-4672-aec4-7f56d9e186b0
Quan, Lanping
75ae7877-e353-41d9-ba4b-c3ad47e5a69c
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Zhou, Cuiqi
a79cddd5-12f5-41fd-af17-f553062f6d70
Lu, Ning
8d60125f-c459-4a0e-8d87-c8e0123d40a5
Wang, Quanhong
4d568c81-ffd2-4d70-b997-5ec4e5570b48
Zhu, Hongxia
34c74461-aab8-4886-8ed4-f80438fa2083
Xu, Ningzhi
7e2ddae3-ef56-4e9e-9ccb-4fddbcdf045f
Zhang, Guo
7bce7bd5-04a3-48ac-8330-6f3521fab734
Zhou, Xiaobo
0deca180-bc8a-42d6-b6a6-237e04cae0e1
Xue, Liyan
d396faa2-726e-4672-aec4-7f56d9e186b0
Quan, Lanping
75ae7877-e353-41d9-ba4b-c3ad47e5a69c
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Zhou, Cuiqi
a79cddd5-12f5-41fd-af17-f553062f6d70
Lu, Ning
8d60125f-c459-4a0e-8d87-c8e0123d40a5
Wang, Quanhong
4d568c81-ffd2-4d70-b997-5ec4e5570b48
Zhu, Hongxia
34c74461-aab8-4886-8ed4-f80438fa2083
Xu, Ningzhi
7e2ddae3-ef56-4e9e-9ccb-4fddbcdf045f

Zhang, Guo, Zhou, Xiaobo, Xue, Liyan, Quan, Lanping, Wang, Yihua, Zhou, Cuiqi, Lu, Ning, Wang, Quanhong, Zhu, Hongxia and Xu, Ningzhi (2005) Accumulation of cytoplasmic beta-catenin correlates with reduced expression of E-cadherin, but not with phosphorylated Akt in esophageal squamous cell carcinoma: immunohistochemical study. Pathology International, 55 (6), 310-317. (doi:10.1111/j.1440-1827.2005.01840.x). (PMID:15943787)

Record type: Article

Abstract

Accumulation of ?-catenin in cytoplasm occurs frequently during the pathogenesis of esophageal squamous cell carcinoma (ESCC). The mechanism leading to this alteration, however, is largely unknown. In the present study, immunohistochemistry was performed for ?-catenin, E-cadherin and Ser473 phosphorylated Akt (P-Akt) in 44 tissue samples of ESCC and corresponding normal esophageal epithelium. Exon 3 of the ?-catenin gene was analyzed by using single-strand conformation polymorphism and direct sequencing. In addition to the reduced expression of E-cadherin and membranous ?-catenin observed in 65.9% and 68% of ESCC tested, respectively, cytoplasmic accumulation of ?-catenin was also detected in 68% (30/44) cases. However, only two cases were found to have the same ?-catenin gene mutation. The data showed that cytoplasmic accumulation of??-catenin was significantly associated ?with? reduced? expression? of? E-cadherin? (P < 0.05) and that of membranous ?-catenin (P < 0.05). Furthermore, cytoplasmic ?-catenin was correlated significantly with lymph node metastasis (P < 0.05). In contrast, although strong staining of P-Akt occurred in 14 of 44 cases (32%), there was no significant correlation between the positive staining of P-Akt and cytoplasmic ?-catenin. Taken together these results suggest that the lost membranous ?-catenin might translocate to cytoplasm depending on reduced expression of E-cadherin, while Akt seems unlikely to play a role in this process.

Full text not available from this repository.

More information

Accepted/In Press date: 18 February 2005
Published date: June 2005
Keywords: ?-catenin, E-cadherin, esophagus, immunohistochemistry, P-Akt, squamous cell carcinoma
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 385917
URI: http://eprints.soton.ac.uk/id/eprint/385917
ISSN: 1320-5463
PURE UUID: 86c15431-df43-4195-a024-c98244181919
ORCID for Yihua Wang: ORCID iD orcid.org/0000-0001-5561-0648

Catalogue record

Date deposited: 26 Jan 2016 11:22
Last modified: 20 Jul 2019 00:34

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×