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Downregulation of survivin by RNAi inhibits the growth of esophageal carcinoma cells.

Downregulation of survivin by RNAi inhibits the growth of esophageal carcinoma cells.
Downregulation of survivin by RNAi inhibits the growth of esophageal carcinoma cells.
Esophageal squamous cell carcinoma ranks among one of the most frequent cause of cancer death in the world. Understanding of the molecular mechanisms involved in the pathogenesis of esophageal cancer becomes critical to develop more effective treatments. Elevated expression of survivin in esophageal carcinoma has been reported before and suppression of survivin expression leads to many tumor cells growth inhibition. We hypothesized that downregulation of survivin would inhibit the growth of human esophageal cancer cells. RNA interference directed against survivin was introduced into a human esophageal squamous cell carcinoma cell line KYSE510. Stable clones were selected and western blot analysis was performed to detect the protein level of survivin. Tumor cell growth in vitro and in vivo was assessed by trypan blue exclusion and nude mice experiments. Annexin V/propidium iodide staining followed by flow cytometric analysis and TUNEL assay were used to detect apoptosis in cell culture and in nude mice. We found that RNA interference could efficiently and stably suppress survivin expression in KYSE510 cells. Downregulation of survivin resulted in significantly inhibition of tumor growth in vitro and in vivo. The mechanism appears to be increased induction of apoptosis. Our results suggest a potential role for the targeting of survivin in the treatments of esophageal carcinoma.
1538-4047
974-978
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Zhu, Hongxia
34c74461-aab8-4886-8ed4-f80438fa2083
Quan, Lanping
75ae7877-e353-41d9-ba4b-c3ad47e5a69c
Zhou, Cuiqi
a79cddd5-12f5-41fd-af17-f553062f6d70
Bai, Jinfeng
66033867-936e-4551-96db-61c624e804f1
Zhang, Guo
7bce7bd5-04a3-48ac-8330-6f3521fab734
Zhan, Qimin
c125182f-9a51-4062-93f0-9a30d98942f1
Xu, Ningzhi
7e2ddae3-ef56-4e9e-9ccb-4fddbcdf045f
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Zhu, Hongxia
34c74461-aab8-4886-8ed4-f80438fa2083
Quan, Lanping
75ae7877-e353-41d9-ba4b-c3ad47e5a69c
Zhou, Cuiqi
a79cddd5-12f5-41fd-af17-f553062f6d70
Bai, Jinfeng
66033867-936e-4551-96db-61c624e804f1
Zhang, Guo
7bce7bd5-04a3-48ac-8330-6f3521fab734
Zhan, Qimin
c125182f-9a51-4062-93f0-9a30d98942f1
Xu, Ningzhi
7e2ddae3-ef56-4e9e-9ccb-4fddbcdf045f

Wang, Yihua, Zhu, Hongxia, Quan, Lanping, Zhou, Cuiqi, Bai, Jinfeng, Zhang, Guo, Zhan, Qimin and Xu, Ningzhi (2005) Downregulation of survivin by RNAi inhibits the growth of esophageal carcinoma cells. Cancer Biology & Therapy, 4 (9), 974-978. (doi:10.4161/cbt.4.9.1914). (PMID:16082195)

Record type: Article

Abstract

Esophageal squamous cell carcinoma ranks among one of the most frequent cause of cancer death in the world. Understanding of the molecular mechanisms involved in the pathogenesis of esophageal cancer becomes critical to develop more effective treatments. Elevated expression of survivin in esophageal carcinoma has been reported before and suppression of survivin expression leads to many tumor cells growth inhibition. We hypothesized that downregulation of survivin would inhibit the growth of human esophageal cancer cells. RNA interference directed against survivin was introduced into a human esophageal squamous cell carcinoma cell line KYSE510. Stable clones were selected and western blot analysis was performed to detect the protein level of survivin. Tumor cell growth in vitro and in vivo was assessed by trypan blue exclusion and nude mice experiments. Annexin V/propidium iodide staining followed by flow cytometric analysis and TUNEL assay were used to detect apoptosis in cell culture and in nude mice. We found that RNA interference could efficiently and stably suppress survivin expression in KYSE510 cells. Downregulation of survivin resulted in significantly inhibition of tumor growth in vitro and in vivo. The mechanism appears to be increased induction of apoptosis. Our results suggest a potential role for the targeting of survivin in the treatments of esophageal carcinoma.

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More information

Accepted/In Press date: 13 June 2005
e-pub ahead of print date: 23 June 2005
Published date: September 2005
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 385920
URI: http://eprints.soton.ac.uk/id/eprint/385920
ISSN: 1538-4047
PURE UUID: c1b62597-25af-420f-a2df-ddedde37278b
ORCID for Yihua Wang: ORCID iD orcid.org/0000-0001-5561-0648

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Date deposited: 26 Jan 2016 11:31
Last modified: 17 Dec 2019 01:33

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Contributors

Author: Yihua Wang ORCID iD
Author: Hongxia Zhu
Author: Lanping Quan
Author: Cuiqi Zhou
Author: Jinfeng Bai
Author: Guo Zhang
Author: Qimin Zhan
Author: Ningzhi Xu

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