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Streptococcus mutans biofilm transient viscoelastic fluid behaviour during high-velocity microsprays

Streptococcus mutans biofilm transient viscoelastic fluid behaviour during high-velocity microsprays
Streptococcus mutans biofilm transient viscoelastic fluid behaviour during high-velocity microsprays
Using high-speed imaging we assessed Streptococcus mutans biofilm–fluid interactions during exposure to a 60-ms microspray burst with a maximum exit velocity of 51 m/s. S. mutans UA159 biofilms were grown for 72 h on 10 mm-length glass slides pre-conditioned with porcine gastric mucin. Biofilm stiffness was measured by performing uniaxial-compression tests. We developed an in-vitro interproximal model which allowed the parallel insertion of two biofilm-colonized slides separated by a distance of 1 mm and enabled high-speed imaging of the removal process at the surface. S. mutans biofilms were exposed to either a water microspray or an air-only microburst. High-speed videos provided further insight into the mechanical behaviour of biofilms as complex liquids and into high-shear fluid–biofilm interaction. We documented biofilms extremely transient fluid behaviour when exposed to the high-velocity microsprays. The presence of time-dependent recoil and residual deformation confirmed the pivotal role of viscoelasticity in biofilm removal. The air-only microburst was effective enough to remove some of the biofilm but created a smaller clearance zone underlying the importance of water and the air–water interface of drops moving over the solid surface in the removal process. Confocal and COMSTAT analysis showed the high-velocity water microspray caused up to a 99.9% reduction in biofilm thickness, biomass and area coverage, within the impact area.
biofilm, oral hygiene, high-speed camera, fluid dynamics, mechanical properties, viscoelasticity
1751-6161
197-206
Fabbri, S.
c93b6166-2117-48a9-9a88-b23a62c7b5da
Johnston, D.A.
b41163c9-b9d2-425c-af99-2a357204014e
Rmaile, A.
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Gottenbos, B.
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De Jager, M.
97b2b3cb-02d5-4c02-9f12-49627b85b57c
Aspiras, M.
198433cd-5075-49d0-ac43-c305c35dc4f3
Starke, M.E.
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Ward, M.T.
21684a17-3051-43f9-bd3f-7adb147f22ab
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f
Fabbri, S.
c93b6166-2117-48a9-9a88-b23a62c7b5da
Johnston, D.A.
b41163c9-b9d2-425c-af99-2a357204014e
Rmaile, A.
d6bb51ea-0892-465c-a2fe-5e530bc6330a
Gottenbos, B.
0b14ede8-eb0f-461c-a520-d36f290a4a08
De Jager, M.
97b2b3cb-02d5-4c02-9f12-49627b85b57c
Aspiras, M.
198433cd-5075-49d0-ac43-c305c35dc4f3
Starke, M.E.
7f1b0bc3-77d6-4ada-8dd6-fd161465ee63
Ward, M.T.
21684a17-3051-43f9-bd3f-7adb147f22ab
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f

Fabbri, S., Johnston, D.A., Rmaile, A., Gottenbos, B., De Jager, M., Aspiras, M., Starke, M.E., Ward, M.T. and Stoodley, P. (2016) Streptococcus mutans biofilm transient viscoelastic fluid behaviour during high-velocity microsprays. Journal of the Mechanical Behavior of Biomedical Materials, 59, 197-206. (doi:10.1016/j.jmbbm.2015.12.012). (PMID:26771168)

Record type: Article

Abstract

Using high-speed imaging we assessed Streptococcus mutans biofilm–fluid interactions during exposure to a 60-ms microspray burst with a maximum exit velocity of 51 m/s. S. mutans UA159 biofilms were grown for 72 h on 10 mm-length glass slides pre-conditioned with porcine gastric mucin. Biofilm stiffness was measured by performing uniaxial-compression tests. We developed an in-vitro interproximal model which allowed the parallel insertion of two biofilm-colonized slides separated by a distance of 1 mm and enabled high-speed imaging of the removal process at the surface. S. mutans biofilms were exposed to either a water microspray or an air-only microburst. High-speed videos provided further insight into the mechanical behaviour of biofilms as complex liquids and into high-shear fluid–biofilm interaction. We documented biofilms extremely transient fluid behaviour when exposed to the high-velocity microsprays. The presence of time-dependent recoil and residual deformation confirmed the pivotal role of viscoelasticity in biofilm removal. The air-only microburst was effective enough to remove some of the biofilm but created a smaller clearance zone underlying the importance of water and the air–water interface of drops moving over the solid surface in the removal process. Confocal and COMSTAT analysis showed the high-velocity water microspray caused up to a 99.9% reduction in biofilm thickness, biomass and area coverage, within the impact area.

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Accepted/In Press date: 14 December 2015
e-pub ahead of print date: 23 December 2015
Published date: June 2016
Keywords: biofilm, oral hygiene, high-speed camera, fluid dynamics, mechanical properties, viscoelasticity
Organisations: Bioengineering Group, Engineering Science Unit, nCATS Group, Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 385961
URI: http://eprints.soton.ac.uk/id/eprint/385961
ISSN: 1751-6161
PURE UUID: 02ef03de-14e7-47a6-89a6-a810e987f3b8
ORCID for D.A. Johnston: ORCID iD orcid.org/0000-0001-6703-6014
ORCID for P. Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 27 Jan 2016 08:58
Last modified: 17 Dec 2019 01:44

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Contributors

Author: S. Fabbri
Author: D.A. Johnston ORCID iD
Author: A. Rmaile
Author: B. Gottenbos
Author: M. De Jager
Author: M. Aspiras
Author: M.E. Starke
Author: M.T. Ward
Author: P. Stoodley ORCID iD

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