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Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium

Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium
Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium
Epithelial-neutrophil activating peptide-78 (ENA-78) induces neutrophil migration, an early response to viral infection. Rhinovirus serotype 16 (RV16) was used to infect primary bronchial epithelial cells and a cell line (BEAS-2B). Release of ENA-78 protein was measured by enzyme-linked immunosorbent assay, ENA-78 mRNA production was quantified by reverse-transcription polymerase chain reaction, and ENA-78 promoter activity was assessed by use of a promoter construct. After infection with RV16, ENA-78 protein and mRNA increased significantly, and RV16 induced 3-fold increases in ENA-78 gene transcription. Nasal ENA-78 measured in patients with asthma with and without RV infection was more elevated in patients with RV infection present. Our study demonstrates that ENA-78 is produced in bronchial epithelial cells in response to RV16 infection. With other chemokines, it may be an important initiator of neutrophil airway inflammation during RV common colds and thus may play a role in the development of virus-associated airway pathologies.
0022-1899
1809-1817
Donninger, Howard
ba6b5180-8a3e-402a-8276-c6dd77be6a79
Glashoff, Richard
f83e6733-5f37-46ac-b07b-d6481ae749cb
Haitchi, Hans-Michael
68dadb29-305d-4236-884f-e9c93f4d78fe
Syce, James A.
b3ad37f7-5c3d-49ea-b76f-3515da371e02
Ghildyal, Reena
9385eb4e-f0fc-43c9-b419-b9932de6b3f8
van Rensburg, Estrelita
b04bfdd2-ebab-4400-8b5e-a3cefab5b818
Bardin, Philip G.
cc3dd40f-a88c-4130-94eb-30cef82d2d28
Donninger, Howard
ba6b5180-8a3e-402a-8276-c6dd77be6a79
Glashoff, Richard
f83e6733-5f37-46ac-b07b-d6481ae749cb
Haitchi, Hans-Michael
68dadb29-305d-4236-884f-e9c93f4d78fe
Syce, James A.
b3ad37f7-5c3d-49ea-b76f-3515da371e02
Ghildyal, Reena
9385eb4e-f0fc-43c9-b419-b9932de6b3f8
van Rensburg, Estrelita
b04bfdd2-ebab-4400-8b5e-a3cefab5b818
Bardin, Philip G.
cc3dd40f-a88c-4130-94eb-30cef82d2d28

Donninger, Howard, Glashoff, Richard, Haitchi, Hans-Michael, Syce, James A., Ghildyal, Reena, van Rensburg, Estrelita and Bardin, Philip G. (2003) Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium. The Journal of Infectious Diseases, 187 (11), 1809-1817. (doi:10.1086/375246). (PMID:12751040)

Record type: Article

Abstract

Epithelial-neutrophil activating peptide-78 (ENA-78) induces neutrophil migration, an early response to viral infection. Rhinovirus serotype 16 (RV16) was used to infect primary bronchial epithelial cells and a cell line (BEAS-2B). Release of ENA-78 protein was measured by enzyme-linked immunosorbent assay, ENA-78 mRNA production was quantified by reverse-transcription polymerase chain reaction, and ENA-78 promoter activity was assessed by use of a promoter construct. After infection with RV16, ENA-78 protein and mRNA increased significantly, and RV16 induced 3-fold increases in ENA-78 gene transcription. Nasal ENA-78 measured in patients with asthma with and without RV infection was more elevated in patients with RV infection present. Our study demonstrates that ENA-78 is produced in bronchial epithelial cells in response to RV16 infection. With other chemokines, it may be an important initiator of neutrophil airway inflammation during RV common colds and thus may play a role in the development of virus-associated airway pathologies.

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More information

Accepted/In Press date: 6 January 2003
e-pub ahead of print date: 15 May 2003
Published date: 1 June 2003
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 386159
URI: https://eprints.soton.ac.uk/id/eprint/386159
ISSN: 0022-1899
PURE UUID: ec3b2173-7e72-46fe-aad6-83fabf4663e0
ORCID for Hans-Michael Haitchi: ORCID iD orcid.org/0000-0001-8603-302X

Catalogue record

Date deposited: 28 Jan 2016 16:02
Last modified: 06 Jun 2018 12:47

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Contributors

Author: Howard Donninger
Author: Richard Glashoff
Author: James A. Syce
Author: Reena Ghildyal
Author: Estrelita van Rensburg
Author: Philip G. Bardin

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