The University of Southampton
University of Southampton Institutional Repository

Two subpopulations of Crocosphaera watsonii have distinct distributions in the North and South Pacific

Two subpopulations of Crocosphaera watsonii have distinct distributions in the North and South Pacific
Two subpopulations of Crocosphaera watsonii have distinct distributions in the North and South Pacific
Crocosphaera watsonii is a unicellular nitrogen (N2)-fixing cyanobacterium with ecological importance in oligotrophic oceans. In cultivated strains there are two phenotypes of C.?watsonii (large and small cells) with differences that could differentially impact biogeochemical processes. Recent work has shown the phenotypes diverged through loss or addition of type-specific genes in a fraction of their genomes, whereas the rest of the genomes were maintained at 99–100% DNA identity. Previous molecular assays for C.?watsonii abundances targeted the conserved regions and therefore could not differentiate between phenotypes, so their relative distributions in natural communities were unknown. To determine phenotype distributions, this study developed and applied type-specific quantitative polymerase chain reaction assays to samples from the North and South Pacific. Abundances of both Crocosphaera types declined sharply with depth between 45 and 75?m in both sites. In surface water small cells were 10–100 times more abundant than large cells in the N. Pacific, whereas in the S. Pacific the two phenotypes were nearly equal. Evidence for large cell aggregation was only found in N. Pacific samples. The differences in C.?watsonii sub-populations in the North and South Pacific have direct implications for biogeochemistry and carbon export in oligotrophic gyres.
1462-2920
514-524
Bench, Shellie R.
3af1690f-2f4e-49c0-a747-99a711e3b6e1
Frank, Ildiko
f2e37fa5-988e-406b-8f25-4de2d71db873
Robidart, Julie
a9b8d49c-c1e3-4a3b-a53c-685a0f2c7f93
Zehr, Jonathan P.
df07e726-0814-4247-8a74-942c07bd23e2
Bench, Shellie R.
3af1690f-2f4e-49c0-a747-99a711e3b6e1
Frank, Ildiko
f2e37fa5-988e-406b-8f25-4de2d71db873
Robidart, Julie
a9b8d49c-c1e3-4a3b-a53c-685a0f2c7f93
Zehr, Jonathan P.
df07e726-0814-4247-8a74-942c07bd23e2

Bench, Shellie R., Frank, Ildiko, Robidart, Julie and Zehr, Jonathan P. (2016) Two subpopulations of Crocosphaera watsonii have distinct distributions in the North and South Pacific. Environmental Microbiology, 18 (2), 514-524. (doi:10.1111/1462-2920.13180).

Record type: Article

Abstract

Crocosphaera watsonii is a unicellular nitrogen (N2)-fixing cyanobacterium with ecological importance in oligotrophic oceans. In cultivated strains there are two phenotypes of C.?watsonii (large and small cells) with differences that could differentially impact biogeochemical processes. Recent work has shown the phenotypes diverged through loss or addition of type-specific genes in a fraction of their genomes, whereas the rest of the genomes were maintained at 99–100% DNA identity. Previous molecular assays for C.?watsonii abundances targeted the conserved regions and therefore could not differentiate between phenotypes, so their relative distributions in natural communities were unknown. To determine phenotype distributions, this study developed and applied type-specific quantitative polymerase chain reaction assays to samples from the North and South Pacific. Abundances of both Crocosphaera types declined sharply with depth between 45 and 75?m in both sites. In surface water small cells were 10–100 times more abundant than large cells in the N. Pacific, whereas in the S. Pacific the two phenotypes were nearly equal. Evidence for large cell aggregation was only found in N. Pacific samples. The differences in C.?watsonii sub-populations in the North and South Pacific have direct implications for biogeochemistry and carbon export in oligotrophic gyres.

Text
Bench et al., 2015 Post Print.pdf - Accepted Manuscript
Download (210kB)

More information

e-pub ahead of print date: 20 January 2016
Published date: February 2016
Organisations: Ocean Technology and Engineering

Identifiers

Local EPrints ID: 386450
URI: http://eprints.soton.ac.uk/id/eprint/386450
ISSN: 1462-2920
PURE UUID: ee79a833-e0e7-4331-afd6-7f06378380fe

Catalogue record

Date deposited: 26 Jan 2016 09:39
Last modified: 16 Dec 2019 20:08

Export record

Altmetrics

Contributors

Author: Shellie R. Bench
Author: Ildiko Frank
Author: Julie Robidart
Author: Jonathan P. Zehr

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×