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A polysaccharide from mushroom Huaier retards human hepatocellular carcinoma growth, angiogenesis, and metastasis in nude mice

A polysaccharide from mushroom Huaier retards human hepatocellular carcinoma growth, angiogenesis, and metastasis in nude mice
A polysaccharide from mushroom Huaier retards human hepatocellular carcinoma growth, angiogenesis, and metastasis in nude mice
Mushroom Huaier has become a focus of interest in the treatment of hepatocellular carcinoma (HCC). Presently, we isolated and purified one polysaccharide from this mushroom. This study aimed to investigate the effects of SP1 on tumor growth and metastasis in a HCC xenograft model and explore its possible mechanism of action. Our results showed that SP1 not only significantly inhibited the proliferation of SMMC-7721 cells in vitro at the concentration ranging from 0 to 800 ?g/ml but also suppressed the HCC tumor growth and metastatic nodules to the lung in SMMC-7721-bearing mice by oral administration at three doses of 30, 60, and 120 mg/kg. Concomitantly, immunohistochemistry analysis of tumor tissues identified that SP1 administration at three doses significantly inhibited the in vivo cancer cell proliferation and microvessel density (MVD) formation, evidenced by a low proliferating cell nuclear antigen (PCNA) and CD34 expression, but increased the percentage of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive cells. Keeping in line with this observation, SP1 treatment decreased serum matrix metalloproteinase (MMP) 2 and vascular endothelial growth factor (VEGF) levels, downregulated the protein expression of hypoxia-inducible factor (HIF)-1alpha, VEGF, MMP2, bcl-2, N-cadherin, signal transducer and activator of transcription 3 (STAT3), and metadherin (MTDH), and upregulated bax and NE-cadherin protein expression in tumor tissues. Taken together, our data suggest that SP1 appears to be a promising chemopreventive agent for the tumorigenesis and metastasis in patients with HCC, especially at advanced stages
huaier purified polysaccharide, metastasis, angiogenesis
1010-4283
2929-2936
Zou, Yanmei
6783820d-2ff0-4900-9ade-34fe1180f56f
Xiong, Hua
f80e5b10-9793-40ae-8143-1762577028f4
Xiong, Huihua
c835b64f-d8a2-4a72-8c53-b730db6ded56
Lu, Tao
1658fb42-f32a-416d-86b5-3ab28dda8338
Zhu, Feng
a9499a5c-c42b-4fc0-91dd-0a7f830e6ce0
Luo, Zhiyong
c770d311-3ca9-41de-8ce9-f560a0ed79b1
Yuan, Xianglin
54dfb6d3-78bf-48da-936d-ded37bfcf238
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Zou, Yanmei
6783820d-2ff0-4900-9ade-34fe1180f56f
Xiong, Hua
f80e5b10-9793-40ae-8143-1762577028f4
Xiong, Huihua
c835b64f-d8a2-4a72-8c53-b730db6ded56
Lu, Tao
1658fb42-f32a-416d-86b5-3ab28dda8338
Zhu, Feng
a9499a5c-c42b-4fc0-91dd-0a7f830e6ce0
Luo, Zhiyong
c770d311-3ca9-41de-8ce9-f560a0ed79b1
Yuan, Xianglin
54dfb6d3-78bf-48da-936d-ded37bfcf238
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e

Zou, Yanmei, Xiong, Hua, Xiong, Huihua, Lu, Tao, Zhu, Feng, Luo, Zhiyong, Yuan, Xianglin and Wang, Yihua (2015) A polysaccharide from mushroom Huaier retards human hepatocellular carcinoma growth, angiogenesis, and metastasis in nude mice. Tumor Biology, 36 (4), 2929-2936. (doi:10.1007/s13277-014-2923-8). (PMID:25492485)

Record type: Article

Abstract

Mushroom Huaier has become a focus of interest in the treatment of hepatocellular carcinoma (HCC). Presently, we isolated and purified one polysaccharide from this mushroom. This study aimed to investigate the effects of SP1 on tumor growth and metastasis in a HCC xenograft model and explore its possible mechanism of action. Our results showed that SP1 not only significantly inhibited the proliferation of SMMC-7721 cells in vitro at the concentration ranging from 0 to 800 ?g/ml but also suppressed the HCC tumor growth and metastatic nodules to the lung in SMMC-7721-bearing mice by oral administration at three doses of 30, 60, and 120 mg/kg. Concomitantly, immunohistochemistry analysis of tumor tissues identified that SP1 administration at three doses significantly inhibited the in vivo cancer cell proliferation and microvessel density (MVD) formation, evidenced by a low proliferating cell nuclear antigen (PCNA) and CD34 expression, but increased the percentage of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive cells. Keeping in line with this observation, SP1 treatment decreased serum matrix metalloproteinase (MMP) 2 and vascular endothelial growth factor (VEGF) levels, downregulated the protein expression of hypoxia-inducible factor (HIF)-1alpha, VEGF, MMP2, bcl-2, N-cadherin, signal transducer and activator of transcription 3 (STAT3), and metadherin (MTDH), and upregulated bax and NE-cadherin protein expression in tumor tissues. Taken together, our data suggest that SP1 appears to be a promising chemopreventive agent for the tumorigenesis and metastasis in patients with HCC, especially at advanced stages

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More information

Accepted/In Press date: 28 November 2014
Published date: 2015
Keywords: huaier purified polysaccharide, metastasis, angiogenesis
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 386538
URI: http://eprints.soton.ac.uk/id/eprint/386538
ISSN: 1010-4283
PURE UUID: 78428fc7-a1ec-4478-a840-93156554d1d5
ORCID for Yihua Wang: ORCID iD orcid.org/0000-0001-5561-0648

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Date deposited: 28 Jan 2016 09:33
Last modified: 15 Mar 2024 03:52

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Contributors

Author: Yanmei Zou
Author: Hua Xiong
Author: Huihua Xiong
Author: Tao Lu
Author: Feng Zhu
Author: Zhiyong Luo
Author: Xianglin Yuan
Author: Yihua Wang ORCID iD

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