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Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation

Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation
Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation
Background: The cell membrane is a primary and fundamental player in most cellular processes, and fatty acids form a major structural component of cell membranes. The aim of this study was to compare the membrane fatty acid profiles of different human blood leukocytes and selected cell lines, to identify the effects of in vitro culture on fatty acid profiles, and to test medium supplements for their effect on fatty acid profiles.

Methods: Different classes of leukocytes were isolated from human blood and their membrane fatty acid profiles were analysed and compared. After culturing in vitro immortalised and primary leukocytes, membrane fatty acids were analysed and compared. Finally, different lipid formulations were developed and used for supplementing leukocytes in vitro in an effort to maintain the in vivo fatty acid profile. Descriptive and analytical tests were performed to compare the obtained fatty acid profiles.

Results: Membrane fatty acid profiles of primary human CD4+ T-lymphocytes, CD8+ T-lymphocytes, B-lymphocytes and monocytes differed. Moreover, there were differences among Jurkat, Raji and THP-1 cell lines and the corresponding primary leukocyte classes, as well as between freshly prepared and in vitro cultured primary lymphocytes. A lipid supplement was able to maintain cultured Jurkat cells with a membrane fatty acid profile almost identical to that of the primary CD4+ T-lymphocytes. Finally, variations in the lipid supplement composition enabled the development of Jurkat cells with different membrane fatty acid profiles characterising different physiological or pathological human conditions.

Conclusions: Each leukocyte class has its own specific membrane fatty acid profile in vivo. Cultured primary leukocytes and immortalized leukocytic cells display different membrane fatty acid profiles when compared to their respective in vivo counterparts. The membrane fatty acid composition of cultured cells can be restored to reflect that of the corresponding in vivo condition through use of optimised lipid supplementation. Typical physiological or pathological leukocyte membrane fatty acid profiles can be obtained by tuning in vitro fatty acid supplementation.
leukocytes, lymphocytes, monocytes, fatty acids, lipid supplementation, membrane fatty acid profile, membrane lipid composition, cell cultures
1-13
Poggi, P.
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Mirabella, R.
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Neri, S.
aeab8298-2d94-4957-ab1f-3ee244a0a8b2
Assirelli, E.
4e584e45-ff4d-4f56-abe5-7cf9a17c3bcc
Dolzani, P.
93513245-4106-45f6-8a92-3a22333ba8d0
Mariani, E.
676f83ea-5fed-4a65-a177-ad5fcd02f0ad
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Chatgilialoglu, A.
92b527b1-c936-43ac-8c14-b0bc95d8c37b
Poggi, P.
7ad99c73-0f06-465b-b741-d37a4b626d78
Mirabella, R.
9e1dd3a8-223c-4a1b-8951-76ef96c1e152
Neri, S.
aeab8298-2d94-4957-ab1f-3ee244a0a8b2
Assirelli, E.
4e584e45-ff4d-4f56-abe5-7cf9a17c3bcc
Dolzani, P.
93513245-4106-45f6-8a92-3a22333ba8d0
Mariani, E.
676f83ea-5fed-4a65-a177-ad5fcd02f0ad
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Chatgilialoglu, A.
92b527b1-c936-43ac-8c14-b0bc95d8c37b

Poggi, P., Mirabella, R., Neri, S., Assirelli, E., Dolzani, P., Mariani, E., Calder, P.C. and Chatgilialoglu, A. (2015) Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation. Lipids in Health and Disease, 14 (165), 1-13. (doi:10.1186/s12944-015-0166-3). (PMID:26703000)

Record type: Article

Abstract

Background: The cell membrane is a primary and fundamental player in most cellular processes, and fatty acids form a major structural component of cell membranes. The aim of this study was to compare the membrane fatty acid profiles of different human blood leukocytes and selected cell lines, to identify the effects of in vitro culture on fatty acid profiles, and to test medium supplements for their effect on fatty acid profiles.

Methods: Different classes of leukocytes were isolated from human blood and their membrane fatty acid profiles were analysed and compared. After culturing in vitro immortalised and primary leukocytes, membrane fatty acids were analysed and compared. Finally, different lipid formulations were developed and used for supplementing leukocytes in vitro in an effort to maintain the in vivo fatty acid profile. Descriptive and analytical tests were performed to compare the obtained fatty acid profiles.

Results: Membrane fatty acid profiles of primary human CD4+ T-lymphocytes, CD8+ T-lymphocytes, B-lymphocytes and monocytes differed. Moreover, there were differences among Jurkat, Raji and THP-1 cell lines and the corresponding primary leukocyte classes, as well as between freshly prepared and in vitro cultured primary lymphocytes. A lipid supplement was able to maintain cultured Jurkat cells with a membrane fatty acid profile almost identical to that of the primary CD4+ T-lymphocytes. Finally, variations in the lipid supplement composition enabled the development of Jurkat cells with different membrane fatty acid profiles characterising different physiological or pathological human conditions.

Conclusions: Each leukocyte class has its own specific membrane fatty acid profile in vivo. Cultured primary leukocytes and immortalized leukocytic cells display different membrane fatty acid profiles when compared to their respective in vivo counterparts. The membrane fatty acid composition of cultured cells can be restored to reflect that of the corresponding in vivo condition through use of optimised lipid supplementation. Typical physiological or pathological leukocyte membrane fatty acid profiles can be obtained by tuning in vitro fatty acid supplementation.

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Accepted/In Press date: 11 December 2015
Published date: 24 December 2015
Keywords: leukocytes, lymphocytes, monocytes, fatty acids, lipid supplementation, membrane fatty acid profile, membrane lipid composition, cell cultures
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 386745
URI: http://eprints.soton.ac.uk/id/eprint/386745
PURE UUID: a9521963-ccae-4e96-82cf-081aff91a1e8
ORCID for P.C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 03 Feb 2016 16:36
Last modified: 15 Mar 2024 02:50

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Contributors

Author: P. Poggi
Author: R. Mirabella
Author: S. Neri
Author: E. Assirelli
Author: P. Dolzani
Author: E. Mariani
Author: P.C. Calder ORCID iD
Author: A. Chatgilialoglu

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