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An adapted flow injection analysis method of phosphate for estuarine samples avoiding matrix effects

An adapted flow injection analysis method of phosphate for estuarine samples avoiding matrix effects
An adapted flow injection analysis method of phosphate for estuarine samples avoiding matrix effects

A flow injection analysis (FIA) method, based on the molybdenum blue formation, has been adapted and successfully optimized in order to use it to measure phosphate in estuarine water samples. Matrix effects have been avoided using the highest peak, and the Schlieren effect (common in FIA analysis) has been minimized by injecting the ascorbic acid with the same sulphuric acid concentration as the molybdate reagent. The concentrations of the reagents have been optimized by a Central Composite Design. The measuring range used was 0.02–0.2 mg L? 1 phosphate and both univariate and multivariate regressions have been compared using a random sample, reaching the same concentration (0.065 ± 0.002 mg L? 1). However, the univariate regression showed lower LODs, 7 ?g L? 1 (74 nM) in front of the 10 ?g L? 1 (105 nM) reached by the multivariate one. The effectiveness of the developed method was tested with real samples. For this, low-cost conventional equipment easily available to most laboratories has been used, with a LOD low enough to measure estuary samples, and high repeatability.
Phosphate, Estuarine, Flow injection analysis, Limit of detection
0026-265X
416-421
Kortazar, Leire
f4b66c18-d78f-46d5-8bf0-24c0014f55c9
Alberdi, Sara
465b5f40-5f01-4581-9f3b-959f26834fd8
Tynan, Eithne
dfed4bbd-ea2a-4ff9-81f5-de2af563943b
Fernández, Luis Angel
bcff2a9a-8e7b-4402-b3da-b77023cbd812
Kortazar, Leire
f4b66c18-d78f-46d5-8bf0-24c0014f55c9
Alberdi, Sara
465b5f40-5f01-4581-9f3b-959f26834fd8
Tynan, Eithne
dfed4bbd-ea2a-4ff9-81f5-de2af563943b
Fernández, Luis Angel
bcff2a9a-8e7b-4402-b3da-b77023cbd812

Kortazar, Leire, Alberdi, Sara, Tynan, Eithne and Fernández, Luis Angel (2016) An adapted flow injection analysis method of phosphate for estuarine samples avoiding matrix effects. Microchemical Journal, 124, 416-421. (doi:10.1016/j.microc.2015.09.027).

Record type: Article

Abstract


A flow injection analysis (FIA) method, based on the molybdenum blue formation, has been adapted and successfully optimized in order to use it to measure phosphate in estuarine water samples. Matrix effects have been avoided using the highest peak, and the Schlieren effect (common in FIA analysis) has been minimized by injecting the ascorbic acid with the same sulphuric acid concentration as the molybdate reagent. The concentrations of the reagents have been optimized by a Central Composite Design. The measuring range used was 0.02–0.2 mg L? 1 phosphate and both univariate and multivariate regressions have been compared using a random sample, reaching the same concentration (0.065 ± 0.002 mg L? 1). However, the univariate regression showed lower LODs, 7 ?g L? 1 (74 nM) in front of the 10 ?g L? 1 (105 nM) reached by the multivariate one. The effectiveness of the developed method was tested with real samples. For this, low-cost conventional equipment easily available to most laboratories has been used, with a LOD low enough to measure estuary samples, and high repeatability.

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More information

Published date: January 2016
Keywords: Phosphate, Estuarine, Flow injection analysis, Limit of detection
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 386913
URI: http://eprints.soton.ac.uk/id/eprint/386913
ISSN: 0026-265X
PURE UUID: bc3d7eba-b4eb-411f-a511-e0cfa4e3185d

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Date deposited: 04 Feb 2016 11:50
Last modified: 27 Apr 2022 04:14

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Contributors

Author: Leire Kortazar
Author: Sara Alberdi
Author: Eithne Tynan
Author: Luis Angel Fernández

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