The University of Southampton
University of Southampton Institutional Repository

Tissue engineering strategies in spinal arthrodesis: the development of new models with the potential to promote fusion

Tissue engineering strategies in spinal arthrodesis: the development of new models with the potential to promote fusion
Tissue engineering strategies in spinal arthrodesis: the development of new models with the potential to promote fusion
Skeletal disorders requiring the regeneration or de novo production of bone present considerable reconstructive challenges and are one of the main driving forces for the development of skeletal tissue engineering strategies today. The incorporation of stem cell technology with material science has been pivotal in the design and application of tissue engineering strategies for bone regeneration but requires a detailed understanding of the complex interactions that occur between stem cells, osteoinductive stimuli, osteoconductive constructs and the biomechanical environment. The requirement for tissue engineering strategies in spinal arthrodesis highlights just one example of the clinical imperative for such strategies. Being a field of personal clinical interest, recent tissue engineering strategies employed to promote spinal fusion and the ongoing challenges to successful clinical translation, are considered in greater detail for the purposes of this thesis. The work in this thesis explores the role of two novel tissue engineering strategies to promote bone tissue regeneration and considers the potential application of these strategies for spinal arthrodesis. Firstly, by examining in vitro and ex vivo the effect of a titanium-spray coated nanopatterned surface on skeletal stem cell behaviour and embryonic chick femur development, it attempts to identify whether nanotopography can direct skeletal stem cell differentiation along an osteoblastic lineage in the absence of chemical stimulation, and considers whether the arrangement of nanopits on the substrate surface affects the osteoinductive surface potential. Furthermore, attempts are made to develop a suitable animal model to analyse the effect of nanopatterned titanium-spray coated substrates in vivo. Secondly, by way of an in vitro study, this thesis analyses the effect of varying pore size on the osteoconductive potential of a 3-D printed 100% sintered hydroxyapatite scaffold seeded with skeletal stem cells. This thesis demonstrates the potential that nanotopography and 3-D printed scaffolds offer to tissue engineering strategiesfor skeletal regeneration but also highlights the challenges to clinical translation and the need for a collaborative multidisciplinary approach for future success.
Evans, Nicholas
0982bf3e-b3f5-4682-9238-f362d6471992
Evans, Nicholas
0982bf3e-b3f5-4682-9238-f362d6471992
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dunlop, Douglas
5f8d8b5c-e516-48b8-831f-c6e5529a52cc

(2015) Tissue engineering strategies in spinal arthrodesis: the development of new models with the potential to promote fusion. University of Southampton, Faculty of Medicine, Doctoral Thesis, 256pp.

Record type: Thesis (Doctoral)

Abstract

Skeletal disorders requiring the regeneration or de novo production of bone present considerable reconstructive challenges and are one of the main driving forces for the development of skeletal tissue engineering strategies today. The incorporation of stem cell technology with material science has been pivotal in the design and application of tissue engineering strategies for bone regeneration but requires a detailed understanding of the complex interactions that occur between stem cells, osteoinductive stimuli, osteoconductive constructs and the biomechanical environment. The requirement for tissue engineering strategies in spinal arthrodesis highlights just one example of the clinical imperative for such strategies. Being a field of personal clinical interest, recent tissue engineering strategies employed to promote spinal fusion and the ongoing challenges to successful clinical translation, are considered in greater detail for the purposes of this thesis. The work in this thesis explores the role of two novel tissue engineering strategies to promote bone tissue regeneration and considers the potential application of these strategies for spinal arthrodesis. Firstly, by examining in vitro and ex vivo the effect of a titanium-spray coated nanopatterned surface on skeletal stem cell behaviour and embryonic chick femur development, it attempts to identify whether nanotopography can direct skeletal stem cell differentiation along an osteoblastic lineage in the absence of chemical stimulation, and considers whether the arrangement of nanopits on the substrate surface affects the osteoinductive surface potential. Furthermore, attempts are made to develop a suitable animal model to analyse the effect of nanopatterned titanium-spray coated substrates in vivo. Secondly, by way of an in vitro study, this thesis analyses the effect of varying pore size on the osteoconductive potential of a 3-D printed 100% sintered hydroxyapatite scaffold seeded with skeletal stem cells. This thesis demonstrates the potential that nanotopography and 3-D printed scaffolds offer to tissue engineering strategiesfor skeletal regeneration but also highlights the challenges to clinical translation and the need for a collaborative multidisciplinary approach for future success.

Text
NICK EVANS - MD THESIS.pdf - Other
Download (188MB)

More information

Published date: June 2015
Organisations: University of Southampton, Human Development & Health

Identifiers

Local EPrints ID: 386941
URI: http://eprints.soton.ac.uk/id/eprint/386941
PURE UUID: 87881981-7ca9-41ac-9e25-d2406d9fb34d
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 17 Feb 2016 13:43
Last modified: 06 Jun 2018 12:53

Export record

Contributors

Author: Nicholas Evans
Thesis advisor: Richard Oreffo ORCID iD
Thesis advisor: Douglas Dunlop

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×