The University of Southampton
University of Southampton Institutional Repository

Ammonia oxidizers are pioneer microorganisms in the colonization of new acidic volcanic soils from South of Chile

Ammonia oxidizers are pioneer microorganisms in the colonization of new acidic volcanic soils from South of Chile
Ammonia oxidizers are pioneer microorganisms in the colonization of new acidic volcanic soils from South of Chile
Ammonia oxidation, performed by specialized microorganisms belonging to the Bacteria and Archaea, is the first and most limiting step of soil nitrification. Nitrification has not yet been examined in young volcanic soils. The aim of the present work was to evaluate the abundance and diversity of ammonia-oxidizing bacteria (AOB) and archaea (AOA) in acidic volcanic soils (andisols) of different defined ages to determine their relative contribution to nitrification and soil colonization. Soil was collected from three vegetated sites on Llaima Volcano (Chile) recolonized after lava eruptions in 1640, 1751 and 1957. Quantitative polymerase chain reaction, terminal restriction fragment length polymorphism and clone sequence analyses of the amoA gene were performed for the AOA and AOB communities. All soils showed high nitrification potentials, but they were highest in the younger soils. Archaeal amoA genes outnumbered bacterial amoA genes at all sites, and AOA abundances were found to be proportional to the nitrification potentials. Sequencing indicated the presence of AOA related to Nitrososphaera and Nitrosotalea, and AOB related primarily to Nitrosospira and sporadically to Nitrosomonas. The study showed that both AOA and AOB are early colonizers of andisols, but that AOA outnumber AOB and play an important role in nitrification.
1758-2229
70-79
Hernandez, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Calabi, Marcela
985c02c5-0696-4eb4-9e70-0d77a34f010f
Basualto, Daniel
2eaf9055-bbb5-438a-b82f-c90781de4d78
Conrad, Ralf
b63adcc7-abe3-4e99-9ce6-20f1cc671d96
Hernandez, Marcela
e73477e7-cf3e-4f50-97c8-4494c5b05cd0
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Calabi, Marcela
985c02c5-0696-4eb4-9e70-0d77a34f010f
Basualto, Daniel
2eaf9055-bbb5-438a-b82f-c90781de4d78
Conrad, Ralf
b63adcc7-abe3-4e99-9ce6-20f1cc671d96

Hernandez, Marcela, Dumont, Marc, Calabi, Marcela, Basualto, Daniel and Conrad, Ralf (2014) Ammonia oxidizers are pioneer microorganisms in the colonization of new acidic volcanic soils from South of Chile. Environmental Microbiology Reports, 6 (1), 70-79. (doi:10.1111/1758-2229.12109). (PMID:24596264)

Record type: Article

Abstract

Ammonia oxidation, performed by specialized microorganisms belonging to the Bacteria and Archaea, is the first and most limiting step of soil nitrification. Nitrification has not yet been examined in young volcanic soils. The aim of the present work was to evaluate the abundance and diversity of ammonia-oxidizing bacteria (AOB) and archaea (AOA) in acidic volcanic soils (andisols) of different defined ages to determine their relative contribution to nitrification and soil colonization. Soil was collected from three vegetated sites on Llaima Volcano (Chile) recolonized after lava eruptions in 1640, 1751 and 1957. Quantitative polymerase chain reaction, terminal restriction fragment length polymorphism and clone sequence analyses of the amoA gene were performed for the AOA and AOB communities. All soils showed high nitrification potentials, but they were highest in the younger soils. Archaeal amoA genes outnumbered bacterial amoA genes at all sites, and AOA abundances were found to be proportional to the nitrification potentials. Sequencing indicated the presence of AOA related to Nitrososphaera and Nitrosotalea, and AOB related primarily to Nitrosospira and sporadically to Nitrosomonas. The study showed that both AOA and AOB are early colonizers of andisols, but that AOA outnumber AOB and play an important role in nitrification.

Text
hernandez-et-al-2014.pdf - Version of Record
Restricted to Repository staff only
Request a copy

More information

e-pub ahead of print date: 21 October 2013
Published date: February 2014
Organisations: Centre for Biological Sciences, Environmental

Identifiers

Local EPrints ID: 387931
URI: http://eprints.soton.ac.uk/id/eprint/387931
ISSN: 1758-2229
PURE UUID: 20589a6a-ea4d-45d8-9819-8467891d33a8
ORCID for Marc Dumont: ORCID iD orcid.org/0000-0002-7347-8668

Catalogue record

Date deposited: 09 Jun 2016 15:59
Last modified: 19 Feb 2020 01:35

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×