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Assimilation of acetate by the putative atmospheric methane oxidizers belonging to the USC[alpha] clade

Assimilation of acetate by the putative atmospheric methane oxidizers belonging to the USC[alpha] clade
Assimilation of acetate by the putative atmospheric methane oxidizers belonging to the USC[alpha] clade
Forest soils are a major biological sink for atmospheric methane, yet the identity and physiology of the microorganisms responsible for this process remain unclear. Although members of the upland soil cluster ? (USC?) are assumed to represent methanotrophic bacteria adapted to the oxidation of the trace level of methane in the atmosphere and to be an important sink of this greenhouse gas, so far they have resisted isolation. In particular, the question of whether the atmospheric methane oxidizers are able to obtain all their energy and carbon solely from atmospheric methane still waits to be answered. In this study, we performed stable-isotope probing (SIP) of RNA and DNA to investigate the assimilation of (13) C-methane and (13) C-acetate by USC? in an acidic forest soil. RNA-SIP showed that pmoA mRNA of USC? was not labelled by (13) C of supplemented (13) C methane, although catalysed reporter deposition - fluorescence in situ hybridization (CARD-FISH) targeting pmoA mRNA of USC? detected its expression in the incubated soil. In contrast, incorporation of (13) C-acetate into USC?pmoA mRNA was observed. USC?pmoA genes were not labelled, indicating that they had not grown during the incubation. Our results indicate that the contribution of alternative carbon sources, such as acetate, to the metabolism of the putative atmospheric methane oxidizers in upland forest soils might be substantial.
1462-2920
2692-2701
Pratscher, Jennifer
bdb96445-89b7-418d-a94c-c68e8a53422b
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Conrad, Ralf
b63adcc7-abe3-4e99-9ce6-20f1cc671d96
Pratscher, Jennifer
bdb96445-89b7-418d-a94c-c68e8a53422b
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Conrad, Ralf
b63adcc7-abe3-4e99-9ce6-20f1cc671d96

Pratscher, Jennifer, Dumont, Marc and Conrad, Ralf (2011) Assimilation of acetate by the putative atmospheric methane oxidizers belonging to the USC[alpha] clade. Environmental Microbiology, 13 (10), 2692-2701. (doi:10.1111/j.1462-2920.2011.02537.x). (PMID:21883789)

Record type: Article

Abstract

Forest soils are a major biological sink for atmospheric methane, yet the identity and physiology of the microorganisms responsible for this process remain unclear. Although members of the upland soil cluster ? (USC?) are assumed to represent methanotrophic bacteria adapted to the oxidation of the trace level of methane in the atmosphere and to be an important sink of this greenhouse gas, so far they have resisted isolation. In particular, the question of whether the atmospheric methane oxidizers are able to obtain all their energy and carbon solely from atmospheric methane still waits to be answered. In this study, we performed stable-isotope probing (SIP) of RNA and DNA to investigate the assimilation of (13) C-methane and (13) C-acetate by USC? in an acidic forest soil. RNA-SIP showed that pmoA mRNA of USC? was not labelled by (13) C of supplemented (13) C methane, although catalysed reporter deposition - fluorescence in situ hybridization (CARD-FISH) targeting pmoA mRNA of USC? detected its expression in the incubated soil. In contrast, incorporation of (13) C-acetate into USC?pmoA mRNA was observed. USC?pmoA genes were not labelled, indicating that they had not grown during the incubation. Our results indicate that the contribution of alternative carbon sources, such as acetate, to the metabolism of the putative atmospheric methane oxidizers in upland forest soils might be substantial.

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Accepted/In Press date: 1 June 2011
e-pub ahead of print date: 24 August 2011
Published date: 11 October 2011
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 387935
URI: http://eprints.soton.ac.uk/id/eprint/387935
ISSN: 1462-2920
PURE UUID: 0f3d8f42-90a7-4d2a-beb8-cd1114593f62
ORCID for Marc Dumont: ORCID iD orcid.org/0000-0002-7347-8668

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Date deposited: 13 Jul 2016 15:50
Last modified: 17 Dec 2019 01:33

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