Stable isotope probing — linking microbial identity to function
Stable isotope probing — linking microbial identity to function
Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as 13C, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and 13C-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics
499-504
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Murrell, J. Colin
244a92ff-dbe1-41cf-9e65-baacbc4a90cf
June 2005
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Murrell, J. Colin
244a92ff-dbe1-41cf-9e65-baacbc4a90cf
Dumont, Marc and Murrell, J. Colin
(2005)
Stable isotope probing — linking microbial identity to function.
Nature Reviews Microbiology, 3 (6), .
(doi:10.1038/nrmicro1162).
Abstract
Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as 13C, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and 13C-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics
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Published date: June 2005
Organisations:
Centre for Biological Sciences
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Local EPrints ID: 387954
URI: http://eprints.soton.ac.uk/id/eprint/387954
ISSN: 1740-1526
PURE UUID: e337e76c-bdc0-45fa-9ba0-5b7d7e8f65b1
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Date deposited: 20 Jun 2016 12:50
Last modified: 15 Mar 2024 03:53
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J. Colin Murrell
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