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Community-level analysis: key genes of aerobic methane oxidation

Community-level analysis: key genes of aerobic methane oxidation
Community-level analysis: key genes of aerobic methane oxidation
Aerobic methane?oxidizing bacteria (methanotrophs) are a diverse group of bacteria that are currently represented by 13 recognized genera. They play a major role in the global methane cycle and are widespread in nature with representatives found in soils, freshwater, seawater, freshwater and marine sediments, peat bogs and at extremes of temperature, salinity, and pH. There has been an interest in methanotrophs for their potential in bioremediation processes. Methanotroph diversity and ecology are often studied using the “functional” genes pmoA, mmoX, and mxaF, encoding subunits of the particulate methane monooxygenase, soluble methane monooxygenase, and the methanol dehydrogenase, respectively. This chapter describes methods used to detect and analyze these functional genes
0076-6879
413-427
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Murrell, J. Colin
22f4dcaa-2fab-4b88-9365-0418bae53ec9
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Murrell, J. Colin
22f4dcaa-2fab-4b88-9365-0418bae53ec9

Dumont, Marc and Murrell, J. Colin (2005) Community-level analysis: key genes of aerobic methane oxidation. Methods in Enzymology, 397, 413-427. (doi:10.1016/S0076-6879(05)97025-0).

Record type: Article

Abstract

Aerobic methane?oxidizing bacteria (methanotrophs) are a diverse group of bacteria that are currently represented by 13 recognized genera. They play a major role in the global methane cycle and are widespread in nature with representatives found in soils, freshwater, seawater, freshwater and marine sediments, peat bogs and at extremes of temperature, salinity, and pH. There has been an interest in methanotrophs for their potential in bioremediation processes. Methanotroph diversity and ecology are often studied using the “functional” genes pmoA, mmoX, and mxaF, encoding subunits of the particulate methane monooxygenase, soluble methane monooxygenase, and the methanol dehydrogenase, respectively. This chapter describes methods used to detect and analyze these functional genes

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Published date: 2005
Organisations: Centre for Biological Sciences, Environmental

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Local EPrints ID: 387955
URI: http://eprints.soton.ac.uk/id/eprint/387955
ISSN: 0076-6879
PURE UUID: eb05ffdc-039e-4e9a-a883-380669964429
ORCID for Marc Dumont: ORCID iD orcid.org/0000-0002-7347-8668

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Date deposited: 13 Jun 2016 11:57
Last modified: 17 Dec 2019 01:33

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Contributors

Author: Marc Dumont ORCID iD
Author: J. Colin Murrell

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