Extracellular matrix deposition in engineered micromass cartilage pellet cultures: measurements and modelling
Extracellular matrix deposition in engineered micromass cartilage pellet cultures: measurements and modelling
This article explores possible mechanisms governing extracellular matrix deposition in engineered cartilaginous cell pellets. A theoretical investigation is carried out alongside an experimental study measuring proteoglycan and collagen volume fractions within murine chondrogenic (ATDC-5) cell pellets. The simple mathematical model, which adopts a nutrient-dependent proteoglycan production rate, successfully reproduces the periphery-dominated proteoglycan deposition, characteristic of the growth pattern observed experimentally within pellets after 21 days of culture. The results suggest that this inhomogeneous proteoglycan production is due to nutrient deficiencies at the pellet centre. Our model analysis further indicates that a spatially uniform distribution of proteoglycan matrix could be maintained by initiating the culture process with a smaller-sized pellet. Finally, possible extensions are put forward with an aim to improve the model predictions for the early behaviour, where different mechanisms appear to dominate the matrix production within the pellets.
1-12
Lewis, Miranda C.
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MacArthur, Ben D.
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Tare, Rahul
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Oreffo, Richard O.C.
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Please, Colin P.
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18 February 2016
Lewis, Miranda C.
e2341eb3-5938-495a-b80c-f9fa0e90f1ee
MacArthur, Ben D.
2c0476e7-5d3e-4064-81bb-104e8e88bb6b
Tare, Rahul
587c9db4-e409-4e7c-a02a-677547ab724a
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Please, Colin P.
fe749b2b-8792-4cd2-b656-5252f68280bf
Lewis, Miranda C., MacArthur, Ben D., Tare, Rahul, Oreffo, Richard O.C. and Please, Colin P.
(2016)
Extracellular matrix deposition in engineered micromass cartilage pellet cultures: measurements and modelling.
PLoS ONE, 11 (2), .
(doi:10.1371/journal.pone.0147302).
Abstract
This article explores possible mechanisms governing extracellular matrix deposition in engineered cartilaginous cell pellets. A theoretical investigation is carried out alongside an experimental study measuring proteoglycan and collagen volume fractions within murine chondrogenic (ATDC-5) cell pellets. The simple mathematical model, which adopts a nutrient-dependent proteoglycan production rate, successfully reproduces the periphery-dominated proteoglycan deposition, characteristic of the growth pattern observed experimentally within pellets after 21 days of culture. The results suggest that this inhomogeneous proteoglycan production is due to nutrient deficiencies at the pellet centre. Our model analysis further indicates that a spatially uniform distribution of proteoglycan matrix could be maintained by initiating the culture process with a smaller-sized pellet. Finally, possible extensions are put forward with an aim to improve the model predictions for the early behaviour, where different mechanisms appear to dominate the matrix production within the pellets.
Text
Extracellular Matrix Deposition in Engineered Micromass Cartilage Pellet Cultures - Measurements and Modelling.pdf
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More information
Accepted/In Press date: 2 January 2016
Published date: 18 February 2016
Organisations:
Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences
Identifiers
Local EPrints ID: 388183
URI: http://eprints.soton.ac.uk/id/eprint/388183
ISSN: 1932-6203
PURE UUID: 989f4d0f-8615-4af8-911d-2f0f16ca7308
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Date deposited: 19 Feb 2016 16:42
Last modified: 15 Mar 2024 03:20
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Contributors
Author:
Miranda C. Lewis
Author:
Colin P. Please
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