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Expression and externalization of Annexin 1 in the adrenal gland: structure and function of the adrenal gland in Annexin 1-null mutant mice

Expression and externalization of Annexin 1 in the adrenal gland: structure and function of the adrenal gland in Annexin 1-null mutant mice
Expression and externalization of Annexin 1 in the adrenal gland: structure and function of the adrenal gland in Annexin 1-null mutant mice
Annexin 1 (ANXA1) is a member of the annexin family of phospholipid- and calcium-binding proteins with a well demonstrated role in early delayed (30 min to 3 h) inhibitory feedback of glucocorticoids in the hypothalamus and pituitary gland. This study used adrenal gland tissue from ANXA1-null transgenic mice, in which a beta-galactosidase (beta-Gal) reporter gene was controlled by the ANXA1 promoter, and wild-type control mice to explore the potential role of ANXA1 in adrenal function. RT-PCR and Western blotting revealed strong expression of ANXA1 mRNA and protein in the adrenal gland. Immunofluorescence labeling of ANXA1 in wild-type and beta-Gal expression in ANXA1-null adrenals localized intense staining in the outer perimeter cell layers. Immunogold electron microscopy identified cytoplasmic and nuclear ANXA1 labeling in outer cortical cells and capsular cells. Exposure of adrenal segments in vitro to dexamethasone (0.1 mum, 3 h) caused an increase in the amount of ANXA1 in the intracellular compartment and attached to the surface of the cells. The N-terminal peptide ANXA1(Ac2-26) inhibited corticosterone release. Corticosterone release was significantly greater from ANXA1-null adrenal cells compared with wild type in response to ACTH (10 pm to 5 nm). In contrast, basal and ACTH-stimulated aldosterone release from ANXA1-null adrenal cells was not different from wild type. Morphometry studies demonstrated that ANXA1 null adrenal glands were smaller than wild-type, and the cortical/medullary area ratio was significantly reduced. These results suggest ANXA1 is a regulator of adrenocortical size and corticosterone secretion.
0013-7227
1030-1038
Davies, Evelyn
ccf87cba-603c-4b3a-b17e-df0b526a3635
Omer, Selma
c1a560b1-9b1f-4feb-8cda-555643ff9265
Buckingham, Julia C.
b9208d30-e2eb-4575-8db1-7f0fc0a4602b
Morris, John F.
5cb2036a-d7e1-4915-8d14-f476b12217ed
Christian, Helen C.
98497ac9-4d3a-4350-8283-dcd83a84207f
Davies, Evelyn
ccf87cba-603c-4b3a-b17e-df0b526a3635
Omer, Selma
c1a560b1-9b1f-4feb-8cda-555643ff9265
Buckingham, Julia C.
b9208d30-e2eb-4575-8db1-7f0fc0a4602b
Morris, John F.
5cb2036a-d7e1-4915-8d14-f476b12217ed
Christian, Helen C.
98497ac9-4d3a-4350-8283-dcd83a84207f

Davies, Evelyn, Omer, Selma, Buckingham, Julia C., Morris, John F. and Christian, Helen C. (2007) Expression and externalization of Annexin 1 in the adrenal gland: structure and function of the adrenal gland in Annexin 1-null mutant mice. Endocrinology, 148 (3), 1030-1038. (doi:10.1210/en.2006-0732). (PMID:17158208)

Record type: Article

Abstract

Annexin 1 (ANXA1) is a member of the annexin family of phospholipid- and calcium-binding proteins with a well demonstrated role in early delayed (30 min to 3 h) inhibitory feedback of glucocorticoids in the hypothalamus and pituitary gland. This study used adrenal gland tissue from ANXA1-null transgenic mice, in which a beta-galactosidase (beta-Gal) reporter gene was controlled by the ANXA1 promoter, and wild-type control mice to explore the potential role of ANXA1 in adrenal function. RT-PCR and Western blotting revealed strong expression of ANXA1 mRNA and protein in the adrenal gland. Immunofluorescence labeling of ANXA1 in wild-type and beta-Gal expression in ANXA1-null adrenals localized intense staining in the outer perimeter cell layers. Immunogold electron microscopy identified cytoplasmic and nuclear ANXA1 labeling in outer cortical cells and capsular cells. Exposure of adrenal segments in vitro to dexamethasone (0.1 mum, 3 h) caused an increase in the amount of ANXA1 in the intracellular compartment and attached to the surface of the cells. The N-terminal peptide ANXA1(Ac2-26) inhibited corticosterone release. Corticosterone release was significantly greater from ANXA1-null adrenal cells compared with wild type in response to ACTH (10 pm to 5 nm). In contrast, basal and ACTH-stimulated aldosterone release from ANXA1-null adrenal cells was not different from wild type. Morphometry studies demonstrated that ANXA1 null adrenal glands were smaller than wild-type, and the cortical/medullary area ratio was significantly reduced. These results suggest ANXA1 is a regulator of adrenocortical size and corticosterone secretion.

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Accepted/In Press date: 30 November 2006
Published date: March 2007
Organisations: Medical Education

Identifiers

Local EPrints ID: 388300
URI: http://eprints.soton.ac.uk/id/eprint/388300
ISSN: 0013-7227
PURE UUID: d7c5aca9-35f0-4791-8d9b-5035bdfd8327

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Date deposited: 23 Feb 2016 11:41
Last modified: 14 Mar 2024 22:55

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Contributors

Author: Evelyn Davies
Author: Selma Omer
Author: Julia C. Buckingham
Author: John F. Morris
Author: Helen C. Christian

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