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Lack of Annexin 1 results in an increase in corticotroph number in male but not female mice

Lack of Annexin 1 results in an increase in corticotroph number in male but not female mice
Lack of Annexin 1 results in an increase in corticotroph number in male but not female mice
Annexin 1 (ANXA1) is a member of the annexin family of phospholipid- and calcium-binding proteins with a well demonstrated role in early delayed (30 min to 3 h) inhibitory feedback of glucocorticoids in the pituitary. We have examined corticotrophs in wild-type and ANXA1 knockout mice to determine the effects of lack of ANXA1 in male and female animals. Anterior pituitary tissue from ANXA1 wild-type, heterozygote and null mice was fixed and examined (i) by confocal immunocytochemistry to determine the number of corticotrophs and (ii) by electron microscopy to examine the size, secretory granule population and secretory machinery of corticotrophs. No differences in these parameters were detected in female mice. In male ANXA1 null mice, there were approximately four-fold more corticotrophs than in wild-type animals. However, the corticotrophs in ANXA1 null mice were smaller and had reduced numbers of secretory granules (the reduction in granules paralleled the reduction in cell size). No differences in the numerical density of folliculo-stellate, gonadotroph, lactotroph or somatotroph cells were detected in male ANXA1 null mice. Plasma corticosterone, adrenocorticotrophic hormone (ACTH) and pituitary pro-opiomelanocortin mRNA were unchanged but pituitary ACTH content was increased in male ANXA1 null mice. Interleukin (IL)-6 pituitary content was significantly elevated in male and reduced in female ANXA1 null mice compared to wild-type. In conclusion, these data indicate that ANXA1 deficiency is associated with gender-specific changes in corticotroph number and structure, via direct actions of ANXA1 and/or indirect changes in factors such as IL-6.
corticotrophs, annexin 1 knockout mice, glucocorticoids
0953-8194
835-846
Morris, J.F.
e471889e-fb79-483d-a424-cf55a1754e36
Omer, S.
c1a560b1-9b1f-4feb-8cda-555643ff9265
Davies, E.
0390ad20-8293-4c62-b00b-58b24c926e11
Wang, E.
0183047f-313e-448a-9915-b54b169a894a
John, C.
5c6e0e3c-bc98-4231-a4df-a62031bb266f
Afzal, T.
a38ecb1c-6b8a-4c07-bfcb-db0bf8c2cab6
Wain, S.
cf38fb55-a785-41e3-907c-fb15902f70b5
Buckingham, J.C.
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Flower, R.J.
d8a4a9f1-9d60-44b7-85f9-b11879156d87
Christian, H.C.
6d8fa111-286d-47fc-95d3-bfefc17e5527
Morris, J.F.
e471889e-fb79-483d-a424-cf55a1754e36
Omer, S.
c1a560b1-9b1f-4feb-8cda-555643ff9265
Davies, E.
0390ad20-8293-4c62-b00b-58b24c926e11
Wang, E.
0183047f-313e-448a-9915-b54b169a894a
John, C.
5c6e0e3c-bc98-4231-a4df-a62031bb266f
Afzal, T.
a38ecb1c-6b8a-4c07-bfcb-db0bf8c2cab6
Wain, S.
cf38fb55-a785-41e3-907c-fb15902f70b5
Buckingham, J.C.
97f0c23b-de2d-4327-bbaf-9f7abf317b6e
Flower, R.J.
d8a4a9f1-9d60-44b7-85f9-b11879156d87
Christian, H.C.
6d8fa111-286d-47fc-95d3-bfefc17e5527

Morris, J.F., Omer, S., Davies, E., Wang, E., John, C., Afzal, T., Wain, S., Buckingham, J.C., Flower, R.J. and Christian, H.C. (2006) Lack of Annexin 1 results in an increase in corticotroph number in male but not female mice. Journal of Neuroendocrinology, 18 (11), 835-846. (doi:10.1111/j.1365-2826.2006.01481.x). (PMID:17026533)

Record type: Article

Abstract

Annexin 1 (ANXA1) is a member of the annexin family of phospholipid- and calcium-binding proteins with a well demonstrated role in early delayed (30 min to 3 h) inhibitory feedback of glucocorticoids in the pituitary. We have examined corticotrophs in wild-type and ANXA1 knockout mice to determine the effects of lack of ANXA1 in male and female animals. Anterior pituitary tissue from ANXA1 wild-type, heterozygote and null mice was fixed and examined (i) by confocal immunocytochemistry to determine the number of corticotrophs and (ii) by electron microscopy to examine the size, secretory granule population and secretory machinery of corticotrophs. No differences in these parameters were detected in female mice. In male ANXA1 null mice, there were approximately four-fold more corticotrophs than in wild-type animals. However, the corticotrophs in ANXA1 null mice were smaller and had reduced numbers of secretory granules (the reduction in granules paralleled the reduction in cell size). No differences in the numerical density of folliculo-stellate, gonadotroph, lactotroph or somatotroph cells were detected in male ANXA1 null mice. Plasma corticosterone, adrenocorticotrophic hormone (ACTH) and pituitary pro-opiomelanocortin mRNA were unchanged but pituitary ACTH content was increased in male ANXA1 null mice. Interleukin (IL)-6 pituitary content was significantly elevated in male and reduced in female ANXA1 null mice compared to wild-type. In conclusion, these data indicate that ANXA1 deficiency is associated with gender-specific changes in corticotroph number and structure, via direct actions of ANXA1 and/or indirect changes in factors such as IL-6.

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Accepted/In Press date: 30 July 2006
e-pub ahead of print date: 14 August 2006
Published date: November 2006
Keywords: corticotrophs, annexin 1 knockout mice, glucocorticoids
Organisations: Faculty of Medicine

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Local EPrints ID: 388301
URI: http://eprints.soton.ac.uk/id/eprint/388301
ISSN: 0953-8194
PURE UUID: 43f8b00a-0e63-4c54-887c-7ad1cb99f8cc

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Date deposited: 23 Feb 2016 11:46
Last modified: 15 Jul 2019 20:45

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Contributors

Author: J.F. Morris
Author: S. Omer
Author: E. Davies
Author: E. Wang
Author: C. John
Author: T. Afzal
Author: S. Wain
Author: J.C. Buckingham
Author: R.J. Flower
Author: H.C. Christian

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