The University of Southampton
University of Southampton Institutional Repository

Laser direct write techniques for the fabrication of paper-based diagnostic devices

Laser direct write techniques for the fabrication of paper-based diagnostic devices
Laser direct write techniques for the fabrication of paper-based diagnostic devices
We report on the use of laser direct-write techniques for the fabrication of point-of-care paper-based diagnostic sensors. These include laser-based deposition, laser ablation and laser-induced photo-polymerisation.

Firstly, Laser Induced Forward Transfer (LIFT) was employed to deposit biomolecules from a donor film onto paper receivers. Paper was chosen as the ideal receiver because of its inherent properties which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged antibodies were LIFT-printed and their viability was confirmed via a colorimetric enzyme-linked immunosorbent assay (ELISA).

Secondly, we report on the laser-based structuring of paper-based fluidic devices. Laser-scanning the paper defines the areas that will be polymerised, thus creating barriers that keep the liquid solutions contained. Complicated devices are easy to fabricate and the flexibility of this technique allows for unique patterns, making it appropriate for rapid prototyping but also for large-scale production. Furthermore, the laser patterning technique allows control of the depth or degree of polymerisation, thereby allowing the liquid to wick through but also imposition of flow delays.

Finally, the use of lasers for the fabrication of a 'master' which can be used for casting a PDMS mould for applications in micro-contact printing. The combination of the above mentioned techniques represent the platform technology for the rapid, precise and versatile laser-based fabrication of diagnostic point-of-care sensors.
Katis, Ioannis
f92dfb8f-610d-4877-83f6-fd26a571df12
Katis, Ioannis
f92dfb8f-610d-4877-83f6-fd26a571df12
Eason, Robert
e38684c3-d18c-41b9-a4aa-def67283b020

Katis, Ioannis (2015) Laser direct write techniques for the fabrication of paper-based diagnostic devices. University of Southampton, Physical Sciences and Engineering, Doctoral Thesis, 181pp.

Record type: Thesis (Doctoral)

Abstract

We report on the use of laser direct-write techniques for the fabrication of point-of-care paper-based diagnostic sensors. These include laser-based deposition, laser ablation and laser-induced photo-polymerisation.

Firstly, Laser Induced Forward Transfer (LIFT) was employed to deposit biomolecules from a donor film onto paper receivers. Paper was chosen as the ideal receiver because of its inherent properties which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged antibodies were LIFT-printed and their viability was confirmed via a colorimetric enzyme-linked immunosorbent assay (ELISA).

Secondly, we report on the laser-based structuring of paper-based fluidic devices. Laser-scanning the paper defines the areas that will be polymerised, thus creating barriers that keep the liquid solutions contained. Complicated devices are easy to fabricate and the flexibility of this technique allows for unique patterns, making it appropriate for rapid prototyping but also for large-scale production. Furthermore, the laser patterning technique allows control of the depth or degree of polymerisation, thereby allowing the liquid to wick through but also imposition of flow delays.

Finally, the use of lasers for the fabrication of a 'master' which can be used for casting a PDMS mould for applications in micro-contact printing. The combination of the above mentioned techniques represent the platform technology for the rapid, precise and versatile laser-based fabrication of diagnostic point-of-care sensors.

Text
Thesis_Ioannis_Katis.pdf - Other
Download (32MB)

More information

Published date: December 2015
Organisations: University of Southampton, Optoelectronics Research Centre

Identifiers

Local EPrints ID: 388397
URI: https://eprints.soton.ac.uk/id/eprint/388397
PURE UUID: 52d12206-6c1e-441d-9baa-aadef95bdf36
ORCID for Ioannis Katis: ORCID iD orcid.org/0000-0002-2016-557X
ORCID for Robert Eason: ORCID iD orcid.org/0000-0001-9704-2204

Catalogue record

Date deposited: 01 Mar 2016 12:13
Last modified: 29 Jan 2019 01:38

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×