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Laser direct write techniques for the fabrication of paper-based diagnostic devices

Laser direct write techniques for the fabrication of paper-based diagnostic devices
Laser direct write techniques for the fabrication of paper-based diagnostic devices
We report on the use of laser direct-write techniques for the fabrication of point-of-care paper-based diagnostic sensors. These include laser-based deposition, laser ablation and laser-induced photo-polymerisation.

Firstly, Laser Induced Forward Transfer (LIFT) was employed to deposit biomolecules from a donor film onto paper receivers. Paper was chosen as the ideal receiver because of its inherent properties which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged antibodies were LIFT-printed and their viability was confirmed via a colorimetric enzyme-linked immunosorbent assay (ELISA).

Secondly, we report on the laser-based structuring of paper-based fluidic devices. Laser-scanning the paper defines the areas that will be polymerised, thus creating barriers that keep the liquid solutions contained. Complicated devices are easy to fabricate and the flexibility of this technique allows for unique patterns, making it appropriate for rapid prototyping but also for large-scale production. Furthermore, the laser patterning technique allows control of the depth or degree of polymerisation, thereby allowing the liquid to wick through but also imposition of flow delays.

Finally, the use of lasers for the fabrication of a 'master' which can be used for casting a PDMS mould for applications in micro-contact printing. The combination of the above mentioned techniques represent the platform technology for the rapid, precise and versatile laser-based fabrication of diagnostic point-of-care sensors.
Katis, Ioannis
f92dfb8f-610d-4877-83f6-fd26a571df12
Katis, Ioannis
f92dfb8f-610d-4877-83f6-fd26a571df12
Eason, Robert
e38684c3-d18c-41b9-a4aa-def67283b020

Katis, Ioannis (2015) Laser direct write techniques for the fabrication of paper-based diagnostic devices. University of Southampton, Physical Sciences and Engineering, Doctoral Thesis, 181pp.

Record type: Thesis (Doctoral)

Abstract

We report on the use of laser direct-write techniques for the fabrication of point-of-care paper-based diagnostic sensors. These include laser-based deposition, laser ablation and laser-induced photo-polymerisation.

Firstly, Laser Induced Forward Transfer (LIFT) was employed to deposit biomolecules from a donor film onto paper receivers. Paper was chosen as the ideal receiver because of its inherent properties which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged antibodies were LIFT-printed and their viability was confirmed via a colorimetric enzyme-linked immunosorbent assay (ELISA).

Secondly, we report on the laser-based structuring of paper-based fluidic devices. Laser-scanning the paper defines the areas that will be polymerised, thus creating barriers that keep the liquid solutions contained. Complicated devices are easy to fabricate and the flexibility of this technique allows for unique patterns, making it appropriate for rapid prototyping but also for large-scale production. Furthermore, the laser patterning technique allows control of the depth or degree of polymerisation, thereby allowing the liquid to wick through but also imposition of flow delays.

Finally, the use of lasers for the fabrication of a 'master' which can be used for casting a PDMS mould for applications in micro-contact printing. The combination of the above mentioned techniques represent the platform technology for the rapid, precise and versatile laser-based fabrication of diagnostic point-of-care sensors.

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More information

Published date: December 2015
Organisations: University of Southampton, Optoelectronics Research Centre

Identifiers

Local EPrints ID: 388397
URI: http://eprints.soton.ac.uk/id/eprint/388397
PURE UUID: 52d12206-6c1e-441d-9baa-aadef95bdf36
ORCID for Ioannis Katis: ORCID iD orcid.org/0000-0002-2016-557X
ORCID for Robert Eason: ORCID iD orcid.org/0000-0001-9704-2204

Catalogue record

Date deposited: 01 Mar 2016 12:13
Last modified: 15 Mar 2024 03:50

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Contributors

Author: Ioannis Katis ORCID iD
Thesis advisor: Robert Eason ORCID iD

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