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High throughput imaging cytometer with acoustic focussing

High throughput imaging cytometer with acoustic focussing
High throughput imaging cytometer with acoustic focussing
We demonstrate an imaging flow cytometer that uses acoustic levitation to assemble cells and other particles into a sheet structure. This technique enables a high resolution, low noise CMOS camera to capture images of thousands of cells with each frame. While ultrasonic focussing has previously been demonstrated for 1D cytometry systems, extending the technology to a planar, much higher throughput format and integrating imaging is non-trivial, and represents a significant jump forward in capability, leading to diagnostic possibilities not achievable with current systems. A galvo mirror is used to track the images of the moving cells permitting exposure times of 10 ms at frame rates of 50 fps with motion blur of only a few pixels. At 80 fps, we demonstrate a throughput of 208 000 beads per second. We investigate the factors affecting motion blur and throughput, and demonstrate the system with fluorescent beads, leukaemia cells and a chondrocyte cell line. Cells require more time to reach the acoustic focus than beads, resulting in lower throughputs; however a longer device would remove this constraint.
2046-2069
83206-83216
Zmijan, Robert
511e2a0d-a3a9-4951-9f57-8852c5824611
Jonnalagadda, Umesh S.
08edef61-4ff5-42fa-a9b2-163bdb277237
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Kochi, Yu
98f40846-73d9-4759-9099-dffe66ec2fae
Lemm, Elizabeth
3db962f5-4499-41fb-a491-bc558b5f45df
Packham, Graham
fdabe56f-2c58-469c-aadf-38878f233394
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Zmijan, Robert
511e2a0d-a3a9-4951-9f57-8852c5824611
Jonnalagadda, Umesh S.
08edef61-4ff5-42fa-a9b2-163bdb277237
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Kochi, Yu
98f40846-73d9-4759-9099-dffe66ec2fae
Lemm, Elizabeth
3db962f5-4499-41fb-a491-bc558b5f45df
Packham, Graham
fdabe56f-2c58-469c-aadf-38878f233394
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d

Zmijan, Robert, Jonnalagadda, Umesh S., Carugo, Dario, Kochi, Yu, Lemm, Elizabeth, Packham, Graham, Hill, Martyn and Glynne-Jones, Peter (2015) High throughput imaging cytometer with acoustic focussing. RSC Advances, 5 (101), 83206-83216. (doi:10.1039/C5RA19497K).

Record type: Article

Abstract

We demonstrate an imaging flow cytometer that uses acoustic levitation to assemble cells and other particles into a sheet structure. This technique enables a high resolution, low noise CMOS camera to capture images of thousands of cells with each frame. While ultrasonic focussing has previously been demonstrated for 1D cytometry systems, extending the technology to a planar, much higher throughput format and integrating imaging is non-trivial, and represents a significant jump forward in capability, leading to diagnostic possibilities not achievable with current systems. A galvo mirror is used to track the images of the moving cells permitting exposure times of 10 ms at frame rates of 50 fps with motion blur of only a few pixels. At 80 fps, we demonstrate a throughput of 208 000 beads per second. We investigate the factors affecting motion blur and throughput, and demonstrate the system with fluorescent beads, leukaemia cells and a chondrocyte cell line. Cells require more time to reach the acoustic focus than beads, resulting in lower throughputs; however a longer device would remove this constraint.

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Accepted/In Press date: 23 September 2015
Published date: 24 September 2015
Organisations: Cancer Sciences, Bioengineering Group, Mechatronics, Engineering Science Unit

Identifiers

Local EPrints ID: 389370
URI: http://eprints.soton.ac.uk/id/eprint/389370
ISSN: 2046-2069
PURE UUID: 7b369ba7-248b-4aed-820f-920299242bec
ORCID for Graham Packham: ORCID iD orcid.org/0000-0002-9232-5691
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953

Catalogue record

Date deposited: 07 Mar 2016 11:45
Last modified: 15 Mar 2024 03:05

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Contributors

Author: Robert Zmijan
Author: Umesh S. Jonnalagadda
Author: Dario Carugo
Author: Yu Kochi
Author: Elizabeth Lemm
Author: Graham Packham ORCID iD
Author: Martyn Hill ORCID iD

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