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Biologically and acoustically compatible chamber for studying ultrasound-mediated delivery of therapeutic compounds

Biologically and acoustically compatible chamber for studying ultrasound-mediated delivery of therapeutic compounds
Biologically and acoustically compatible chamber for studying ultrasound-mediated delivery of therapeutic compounds
Ultrasound (US), in combination with microbubbles, has been found to be a potential alternative to viral therapies for transfecting biological cells. The translation of this technique to the clinical environment, however, requires robust and systematic optimization of the acoustic parameters needed to achieve a desired therapeutic effect. Currently, a variety of different devices have been developed to transfect cells in vitro, resulting in a lack of standardized experimental conditions and difficulty in comparing results from different laboratories. To overcome this limitation, we propose an easy-to-fabricate and cost-effective device for application in US-mediated delivery of therapeutic compounds. It comprises a commercially available cell culture dish coupled with a silicon-based "lid" developed in-house that enables the device to be immersed in a water bath for US exposure. Described here are the design of the device, characterization of the sound field and fluid dynamics inside the chamber and an example protocol for a therapeutic delivery experiment.
cell culture device, in vitro assay, small interfering rna, sonoporation, ultrasound-mediated drug delivery
0301-5629
1927-1937
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Owen, Joshua
b5d9c311-b8d4-40db-b937-65a769a11b4f
Crake, Calum
ab3eeead-4851-4c54-bea9-383cf136c4bb
Lee, Jeong Yu
25c5c704-f4ad-48ea-a984-088034dc3a93
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Owen, Joshua
b5d9c311-b8d4-40db-b937-65a769a11b4f
Crake, Calum
ab3eeead-4851-4c54-bea9-383cf136c4bb
Lee, Jeong Yu
25c5c704-f4ad-48ea-a984-088034dc3a93
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6

Carugo, Dario, Owen, Joshua, Crake, Calum, Lee, Jeong Yu and Stride, Eleanor (2015) Biologically and acoustically compatible chamber for studying ultrasound-mediated delivery of therapeutic compounds. Ultrasound in Medicine & Biology, 41 (7), 1927-1937. (doi:10.1016/j.ultrasmedbio.2015.03.020). (PMID:25922133)

Record type: Article

Abstract

Ultrasound (US), in combination with microbubbles, has been found to be a potential alternative to viral therapies for transfecting biological cells. The translation of this technique to the clinical environment, however, requires robust and systematic optimization of the acoustic parameters needed to achieve a desired therapeutic effect. Currently, a variety of different devices have been developed to transfect cells in vitro, resulting in a lack of standardized experimental conditions and difficulty in comparing results from different laboratories. To overcome this limitation, we propose an easy-to-fabricate and cost-effective device for application in US-mediated delivery of therapeutic compounds. It comprises a commercially available cell culture dish coupled with a silicon-based "lid" developed in-house that enables the device to be immersed in a water bath for US exposure. Described here are the design of the device, characterization of the sound field and fluid dynamics inside the chamber and an example protocol for a therapeutic delivery experiment.

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More information

Accepted/In Press date: 18 March 2015
e-pub ahead of print date: 24 April 2015
Published date: July 2015
Keywords: cell culture device, in vitro assay, small interfering rna, sonoporation, ultrasound-mediated drug delivery
Organisations: Bioengineering Group, Mechatronics, Engineering Science Unit, Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 389481
URI: https://eprints.soton.ac.uk/id/eprint/389481
ISSN: 0301-5629
PURE UUID: f50697a1-6112-428c-bd38-2a9edf0b3da8

Catalogue record

Date deposited: 08 Mar 2016 10:28
Last modified: 16 Jul 2019 20:07

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