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Rapid identification of Saccharomyces eubayanus and its hybrids

Rapid identification of Saccharomyces eubayanus and its hybrids
Rapid identification of Saccharomyces eubayanus and its hybrids
The Saccharomyces species Saccharomyces eubayanus was recently discovered in Patagonia. Genomic, genetic and phylogenetic data all suggest that it is one of the two parents of the hybrid yeast S. pastorianus (S. cerevisiae being the other). Saccharomyces eubayanus genomes can also be found in strains of the hybrid species S. bayanus. Here, we describe a novel pair of PCR primers targeted against the S. eubayanus FSY1 gene that will specifically detect S. eubayanus and hybrids containing this allele of the gene. The primer pair can be used to identify the species using a rapid, inexpensive colony PCR method suitable for a preliminary identification of wild isolates.
Keywords
species, specific promers, colony pcr, multiplex
1567-1356
156-161
Pengelly, Reuben
af97c0c1-b568-415c-9f59-1823b65be76d
Wheals, Alan E.
aad7ff4f-9a79-4729-8eb5-a69cf5777f28
Pengelly, Reuben
af97c0c1-b568-415c-9f59-1823b65be76d
Wheals, Alan E.
aad7ff4f-9a79-4729-8eb5-a69cf5777f28

Pengelly, Reuben and Wheals, Alan E. (2013) Rapid identification of Saccharomyces eubayanus and its hybrids. FEMS Yeast Research, 13 (2), 156-161. (doi:10.1111/1567-1364.12018).

Record type: Article

Abstract

The Saccharomyces species Saccharomyces eubayanus was recently discovered in Patagonia. Genomic, genetic and phylogenetic data all suggest that it is one of the two parents of the hybrid yeast S. pastorianus (S. cerevisiae being the other). Saccharomyces eubayanus genomes can also be found in strains of the hybrid species S. bayanus. Here, we describe a novel pair of PCR primers targeted against the S. eubayanus FSY1 gene that will specifically detect S. eubayanus and hybrids containing this allele of the gene. The primer pair can be used to identify the species using a rapid, inexpensive colony PCR method suitable for a preliminary identification of wild isolates.
Keywords

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More information

Accepted/In Press date: 23 October 2012
Published date: March 2013
Keywords: species, specific promers, colony pcr, multiplex
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 389742
URI: http://eprints.soton.ac.uk/id/eprint/389742
ISSN: 1567-1356
PURE UUID: 0ca63ee7-cfaa-4a12-bc86-1436edfc1b2c
ORCID for Reuben Pengelly: ORCID iD orcid.org/0000-0001-7022-645X

Catalogue record

Date deposited: 14 Mar 2016 14:03
Last modified: 09 Jan 2022 03:45

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Contributors

Author: Reuben Pengelly ORCID iD
Author: Alan E. Wheals

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