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Highly diverse MLVA-ompA genotypes of rectal Chlamydia trachomatis among men who have sex with men in Brighton, UK and evidence for an HIV-related sexual network.

Highly diverse MLVA-ompA genotypes of rectal Chlamydia trachomatis among men who have sex with men in Brighton, UK and evidence for an HIV-related sexual network.
Highly diverse MLVA-ompA genotypes of rectal Chlamydia trachomatis among men who have sex with men in Brighton, UK and evidence for an HIV-related sexual network.
Objectives: In this prospective study, we aimed to determine the distribution of genotypes by multilocus variable number tandem repeat (VNTR) analysis plus analysis of the ompA gene (MLVA-ompA) of rectal Chlamydia trachomatis among men who have sex with men (MSM) attending Brighton Genitourinary Medicine (GUM) Clinic and to examine any correlations with clinical variables, including HIV status, and to isolate rectal C. trachomatis cultures maximising the possibility of obtaining complete genotyping data.

Methods: Samples were assigned genotypes by PCR and sequencing of the markers of the MLVA-ompA genotyping system. Rectal C. trachomatis was isolated in cell culture using McCoy cells. Data regarding demographics, HIV status, rectal symptoms and history of sexually transmitted infections, including C. trachomatis, were collected.

Results: 1809 MSM attending the clinic between October 2011 and January 2013 took part in the study, 112 (6.2%) of whom had rectal samples that tested positive for C. trachomatis. 85/112 (75.9%) C. trachomatis-positive rectal samples were assigned 66 different genotypes. Two distinct genotype subclusters were identified: subcluster 1 consisted of more HIV-negative men than subcluster 2 (p=0.025), and the MLVA-ompA genotypes in these subclusters reflected this. Isolates were successfully cultured from 37 of the 112 specimens, from which 27 otherwise unobtainable (from direct PCR) MLVA-ompA genotypes were gained.

Conclusions: The most prevalent genotypes were G, E and D representing some overlap with the heterosexual distribution in UK. Subcluster 1 consisted of more ‘heterosexual genotypes’ and significantly more HIV-negative men than subcluster 2, associated with ‘MSM genotypes’. There was a higher diversity of C. trachomatis strains among MSM in Brighton than observed in other cities.
1368-4973
Labiran, Clare
7804e65c-6cd6-41a8-b351-abca702f6018
Marsh, Peter
28ca4a02-c63e-45bb-8288-52806daad572
Zhou, Judith
654c4ec0-671c-4e3d-a2d8-b09f099daaa2
Bannister, Alan
4dcafa6e-eeb6-40ea-814d-20343c8bcfee
Clarke, Ian Nicholas
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Goubet, Stephanie
96944744-7267-4285-91c5-b12a5a86d95d
Soni, Suneeta
72b47657-6b1f-49d9-be40-96ec5d3e3ac1
Labiran, Clare
7804e65c-6cd6-41a8-b351-abca702f6018
Marsh, Peter
28ca4a02-c63e-45bb-8288-52806daad572
Zhou, Judith
654c4ec0-671c-4e3d-a2d8-b09f099daaa2
Bannister, Alan
4dcafa6e-eeb6-40ea-814d-20343c8bcfee
Clarke, Ian Nicholas
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Goubet, Stephanie
96944744-7267-4285-91c5-b12a5a86d95d
Soni, Suneeta
72b47657-6b1f-49d9-be40-96ec5d3e3ac1

Labiran, Clare, Marsh, Peter, Zhou, Judith, Bannister, Alan, Clarke, Ian Nicholas, Goubet, Stephanie and Soni, Suneeta (2015) Highly diverse MLVA-ompA genotypes of rectal Chlamydia trachomatis among men who have sex with men in Brighton, UK and evidence for an HIV-related sexual network. Sexually Transmitted Infections. (doi:10.1136/sextrans-2015-052261). (PMID:26487754)

Record type: Article

Abstract

Objectives: In this prospective study, we aimed to determine the distribution of genotypes by multilocus variable number tandem repeat (VNTR) analysis plus analysis of the ompA gene (MLVA-ompA) of rectal Chlamydia trachomatis among men who have sex with men (MSM) attending Brighton Genitourinary Medicine (GUM) Clinic and to examine any correlations with clinical variables, including HIV status, and to isolate rectal C. trachomatis cultures maximising the possibility of obtaining complete genotyping data.

Methods: Samples were assigned genotypes by PCR and sequencing of the markers of the MLVA-ompA genotyping system. Rectal C. trachomatis was isolated in cell culture using McCoy cells. Data regarding demographics, HIV status, rectal symptoms and history of sexually transmitted infections, including C. trachomatis, were collected.

Results: 1809 MSM attending the clinic between October 2011 and January 2013 took part in the study, 112 (6.2%) of whom had rectal samples that tested positive for C. trachomatis. 85/112 (75.9%) C. trachomatis-positive rectal samples were assigned 66 different genotypes. Two distinct genotype subclusters were identified: subcluster 1 consisted of more HIV-negative men than subcluster 2 (p=0.025), and the MLVA-ompA genotypes in these subclusters reflected this. Isolates were successfully cultured from 37 of the 112 specimens, from which 27 otherwise unobtainable (from direct PCR) MLVA-ompA genotypes were gained.

Conclusions: The most prevalent genotypes were G, E and D representing some overlap with the heterosexual distribution in UK. Subcluster 1 consisted of more ‘heterosexual genotypes’ and significantly more HIV-negative men than subcluster 2, associated with ‘MSM genotypes’. There was a higher diversity of C. trachomatis strains among MSM in Brighton than observed in other cities.

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Accepted/In Press date: 26 September 2015
Published date: 20 October 2015
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 389800
URI: https://eprints.soton.ac.uk/id/eprint/389800
ISSN: 1368-4973
PURE UUID: 06f965a1-1b1a-4d62-9474-4d97ea996008
ORCID for Ian Nicholas Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 15 Mar 2016 15:04
Last modified: 06 Jun 2018 13:19

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