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Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology

Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology
Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology
Models of the outer epithelia of the human body - namely the skin, the intestine and the lung - have found valid applications in both research and industrial settings as attractive alternatives to animal testing. A variety of approaches to model these barriers are currently employed in such fields, ranging from the utilization of ex vivo tissue to reconstructed in vitro models, and further to chip-based technologies, synthetic membrane systems and, of increasing current interest, in silico modeling approaches. An international group of experts in the field of epithelial barriers was convened from academia, industry and regulatory bodies to present both the current state of the art of non-animal models of the skin, intestinal and pulmonary barriers in their various fields of application, and to discuss research-based, industry-driven and regulatory-relevant future directions for both the development of new models and the refinement of existing test methods. Issues of model relevance and preference, validation and standardization, acceptance, and the need for simplicity versus complexity were focal themes of the discussions. The outcomes of workshop presentations and discussions, in relation to both current status and future directions in the utilization and development of epithelial barrier models, are presented by the attending experts in the current report.
in vitro models, epithelial cell culture, permeability, transport studies, cytotoxity
1868-596X
327-378
Gordon, Sarah
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Daneshian, Mardas
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Bouwstra, Joke
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Caloni, Francesca
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Constant, Samuel
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Davies, Donna E.
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Dandekar, Gudrun
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Guzman, Carlos A.
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Fabian, Eric
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Haltner, Eleonore
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Hartung, Thomas
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Hasiwa, Nina
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Hayden, Patrick
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Kandarova, Helena
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Khare, Sangeeta
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Krug, Harald F.
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Kneuer, Carsten
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Leist, Marcel
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Lian, Guoping
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Marx, Uwe
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Metzger, Marco
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Ott, Katharina
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Prieto, Pilar
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Roberts, Michael S.
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Roggen, Erwin L.
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Tralau, Tewes
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van den Braak, Claudia
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Walles, Heike
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Lehr, Claus-Michael
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Gordon, Sarah
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Daneshian, Mardas
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Bouwstra, Joke
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Caloni, Francesca
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Constant, Samuel
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Davies, Donna E.
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Dandekar, Gudrun
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Guzman, Carlos A.
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Fabian, Eric
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Haltner, Eleonore
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Hartung, Thomas
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Hasiwa, Nina
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Hayden, Patrick
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Kandarova, Helena
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Khare, Sangeeta
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Krug, Harald F.
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Kneuer, Carsten
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Leist, Marcel
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Lian, Guoping
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Marx, Uwe
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Metzger, Marco
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Ott, Katharina
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Prieto, Pilar
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Roberts, Michael S.
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Roggen, Erwin L.
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Tralau, Tewes
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van den Braak, Claudia
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Walles, Heike
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Lehr, Claus-Michael
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Gordon, Sarah, Daneshian, Mardas, Bouwstra, Joke, Caloni, Francesca, Constant, Samuel, Davies, Donna E., Dandekar, Gudrun, Guzman, Carlos A., Fabian, Eric, Haltner, Eleonore, Hartung, Thomas, Hasiwa, Nina, Hayden, Patrick, Kandarova, Helena, Khare, Sangeeta, Krug, Harald F., Kneuer, Carsten, Leist, Marcel, Lian, Guoping, Marx, Uwe, Metzger, Marco, Ott, Katharina, Prieto, Pilar, Roberts, Michael S., Roggen, Erwin L., Tralau, Tewes, van den Braak, Claudia, Walles, Heike and Lehr, Claus-Michael (2015) Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology. ALTEX, 32 (4), 327-378. (doi:10.14573/altex.1510051). (PMID:26536291)

Record type: Article

Abstract

Models of the outer epithelia of the human body - namely the skin, the intestine and the lung - have found valid applications in both research and industrial settings as attractive alternatives to animal testing. A variety of approaches to model these barriers are currently employed in such fields, ranging from the utilization of ex vivo tissue to reconstructed in vitro models, and further to chip-based technologies, synthetic membrane systems and, of increasing current interest, in silico modeling approaches. An international group of experts in the field of epithelial barriers was convened from academia, industry and regulatory bodies to present both the current state of the art of non-animal models of the skin, intestinal and pulmonary barriers in their various fields of application, and to discuss research-based, industry-driven and regulatory-relevant future directions for both the development of new models and the refinement of existing test methods. Issues of model relevance and preference, validation and standardization, acceptance, and the need for simplicity versus complexity were focal themes of the discussions. The outcomes of workshop presentations and discussions, in relation to both current status and future directions in the utilization and development of epithelial barrier models, are presented by the attending experts in the current report.

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Published date: 2015
Keywords: in vitro models, epithelial cell culture, permeability, transport studies, cytotoxity
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 389842
URI: https://eprints.soton.ac.uk/id/eprint/389842
ISSN: 1868-596X
PURE UUID: f681afe9-ab74-47f6-85d9-158f4cd3519c

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Date deposited: 16 Mar 2016 15:04
Last modified: 17 Jul 2017 19:31

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Contributors

Author: Sarah Gordon
Author: Mardas Daneshian
Author: Joke Bouwstra
Author: Francesca Caloni
Author: Samuel Constant
Author: Donna E. Davies
Author: Gudrun Dandekar
Author: Carlos A. Guzman
Author: Eric Fabian
Author: Eleonore Haltner
Author: Thomas Hartung
Author: Nina Hasiwa
Author: Patrick Hayden
Author: Helena Kandarova
Author: Sangeeta Khare
Author: Harald F. Krug
Author: Carsten Kneuer
Author: Marcel Leist
Author: Guoping Lian
Author: Uwe Marx
Author: Marco Metzger
Author: Katharina Ott
Author: Pilar Prieto
Author: Michael S. Roberts
Author: Erwin L. Roggen
Author: Tewes Tralau
Author: Claudia van den Braak
Author: Heike Walles
Author: Claus-Michael Lehr

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