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Continuous measurement of enzymatic kinetics in droplet flow for point-of-care monitoring

Continuous measurement of enzymatic kinetics in droplet flow for point-of-care monitoring
Continuous measurement of enzymatic kinetics in droplet flow for point-of-care monitoring
Droplet microfluidics is ideally suited to continuous biochemical analysis, requiring low sample volumes and offering high temporal resolution. Many biochemical assays are based on enzymatic reactions, the kinetics of which can be obtained by probing droplets at multiple points over time. Here we present a miniaturised multi-detector flow cell to analyse enzyme kinetics in droplets, with an example application of continuous glucose measurement. Reaction rates and Michaelis–Menten kinetics can be quantified for each individual droplet and unknown glucose concentrations can be accurately determined (errors <5 %). Droplets can be probed continuously giving short sample-to-result time (~30 s) measurement. In contrast to previous reports of multipoint droplet measurement (all of which used bulky microscope-based setups) the flow cell presented here has a small footprint and uses low-powered, low-cost components, making it ideally suited for use in field-deployable devices.
0003-2654
3266-3273
Hassan, Sammer-Ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Nightingale, Adrian
4b51311d-c6c3-40d5-a13f-ab8917031ab3
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa
Hassan, Sammer-Ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Nightingale, Adrian
4b51311d-c6c3-40d5-a13f-ab8917031ab3
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa

Hassan, Sammer-Ul, Nightingale, Adrian and Niu, Xize (2016) Continuous measurement of enzymatic kinetics in droplet flow for point-of-care monitoring. Analyst, 141 (11), 3266-3273. (doi:10.1039/C6AN00620E).

Record type: Article

Abstract

Droplet microfluidics is ideally suited to continuous biochemical analysis, requiring low sample volumes and offering high temporal resolution. Many biochemical assays are based on enzymatic reactions, the kinetics of which can be obtained by probing droplets at multiple points over time. Here we present a miniaturised multi-detector flow cell to analyse enzyme kinetics in droplets, with an example application of continuous glucose measurement. Reaction rates and Michaelis–Menten kinetics can be quantified for each individual droplet and unknown glucose concentrations can be accurately determined (errors <5 %). Droplets can be probed continuously giving short sample-to-result time (~30 s) measurement. In contrast to previous reports of multipoint droplet measurement (all of which used bulky microscope-based setups) the flow cell presented here has a small footprint and uses low-powered, low-cost components, making it ideally suited for use in field-deployable devices.

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Accepted/In Press date: 16 March 2016
e-pub ahead of print date: 16 March 2016
Published date: 7 June 2016
Organisations: Mechatronics

Identifiers

Local EPrints ID: 390187
URI: http://eprints.soton.ac.uk/id/eprint/390187
ISSN: 0003-2654
PURE UUID: 50cfbe3f-c543-49fc-8333-427fec063e74
ORCID for Sammer-Ul Hassan: ORCID iD orcid.org/0000-0002-0319-5814

Catalogue record

Date deposited: 22 Mar 2016 09:17
Last modified: 18 Feb 2021 17:20

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Contributors

Author: Xize Niu

University divisions

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