C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes
C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes
Signals induced by mechanical loading and C-type natriuretic peptide (CNP) represent chondroprotective routes that may potentially prevent osteoarthritis (OA). We examined whether CNP will reduce hyaluronan production and export via members of the multidrug resistance protein (MRP) and diminish pro-inflammatory effects in human chondrocytes. The presence of interleukin-1? (IL-1?) increased HA production and export via MRP5 that was reduced with CNP and/or loading. Treatment with IL-1? conditioned medium increased production of catabolic mediators and the response was reduced with the hyaluronan inhibitor, Pep-1. The induction of pro-inflammatory cytokines by the conditioned medium was reduced by CNP and/or Pep-1, ?CD44 or ?TLR4 in a cytokine-dependent manner, suggesting that the CNP pathway is protective and should be exploited further.
784-789
Peake, N.J.
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Bader, D.L.
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Vessillier, S.
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Ramachandran, M.
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Salter, D.M.
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Hobbs, A.J.
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Chowdhury, T.T.
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2 October 2015
Peake, N.J.
5865b85c-5698-42a3-b54f-fc036ed87e42
Bader, D.L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Vessillier, S.
f4f74639-0815-4664-a163-fd828aff1a10
Ramachandran, M.
132897fa-e457-4bb7-a6e4-3fb2cc18c454
Salter, D.M.
94485705-ebda-4ddf-96c9-55fc6c3fe073
Hobbs, A.J.
a7abebde-319c-439e-a176-dad63401b021
Chowdhury, T.T.
3969bc1d-acec-4cad-b523-c8b4885fcb0a
Peake, N.J., Bader, D.L., Vessillier, S., Ramachandran, M., Salter, D.M., Hobbs, A.J. and Chowdhury, T.T.
(2015)
C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes.
Biochemical and Biophysical Research Communications, 465 (4), .
(doi:10.1016/j.bbrc.2015.08.087).
(PMID:26307537)
Abstract
Signals induced by mechanical loading and C-type natriuretic peptide (CNP) represent chondroprotective routes that may potentially prevent osteoarthritis (OA). We examined whether CNP will reduce hyaluronan production and export via members of the multidrug resistance protein (MRP) and diminish pro-inflammatory effects in human chondrocytes. The presence of interleukin-1? (IL-1?) increased HA production and export via MRP5 that was reduced with CNP and/or loading. Treatment with IL-1? conditioned medium increased production of catabolic mediators and the response was reduced with the hyaluronan inhibitor, Pep-1. The induction of pro-inflammatory cytokines by the conditioned medium was reduced by CNP and/or Pep-1, ?CD44 or ?TLR4 in a cytokine-dependent manner, suggesting that the CNP pathway is protective and should be exploited further.
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Accepted/In Press date: 20 July 2015
e-pub ahead of print date: 22 August 2015
Published date: 2 October 2015
Organisations:
Faculty of Health Sciences
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Local EPrints ID: 390211
URI: http://eprints.soton.ac.uk/id/eprint/390211
ISSN: 0006-291X
PURE UUID: 6e74d590-e48e-4154-b6a8-9141ec616edd
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Date deposited: 22 Mar 2016 11:57
Last modified: 14 Mar 2024 23:13
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Author:
N.J. Peake
Author:
S. Vessillier
Author:
M. Ramachandran
Author:
D.M. Salter
Author:
A.J. Hobbs
Author:
T.T. Chowdhury
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