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A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice

A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice
A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice
Understanding how plants allocate their resources to growth or defence is of long-term importance to the development of new and improved varieties of different crops.
Using molecular genetics, plant physiology, hormone analysis and Next-Generation Sequencing (NGS)-based transcript profiling, we have isolated and characterized the rice(Oryza sativa) LESION AND LAMINA BENDING (LLB) gene that encodes a chloroplast-targeted putative leucine carboxyl methyltransferase.

Loss of LLB function results in reduced growth and yield, hypersensitive response (HR)-like lesions, accumulation of the antimicrobial compounds momilactones and phytocassanes, and constitutive expression of pathogenesis-related genes. Consistent with these defence-associated responses, llb shows enhanced resistance to rice blast (Magnaporthe oryzae) and bacterial blight (Xanthomonas oryzae pv. oryzae). The lesion and resistance phenotypes are likely to be caused by the over-accumulation of jasmonates (JAs) in the llb mutant including the JA precursor 12-oxo-phytodienoic acid. Additionally, llb shows an increased lamina inclination and enhanced early seedling growth due to elevated brassinosteroid (BR) synthesis and/or signalling.

These findings show that LLB functions in the chloroplast to either directly or indirectly repress both JA- and BR-mediated responses, revealing a possible mechanism for controlling how plants allocate resources for defence and growth.
0028-646X
1282-1297
Tamiru, Muluneh
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Takagi, Hiroki
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Abe, Akira
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Yokota, Takao
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Kanzaki, Hiroyuki
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Okamoto, Haruko
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Saitoh, Hiromasa
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Takahashi, Hideyuki
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Fujisaki, Koki
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Oikawa, Kaori
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Uemura, Aiko
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Natsume, Satoshi
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Jikumaru, Yusuke
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Matsuura, Hideyuki
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Umemura, Kenji
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Terry, Matthew J.
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Terauchi, Ryohei
934926b5-7016-4152-aded-1a002da1f66b
Tamiru, Muluneh
1aa4d312-1946-41ce-8506-880061ce6ff0
Takagi, Hiroki
2db1e271-aa87-4fe4-9ec4-d5a9a4017ae8
Abe, Akira
cefcadd9-e3cc-4f42-9cb6-74c19b0dfc2a
Yokota, Takao
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Kanzaki, Hiroyuki
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Okamoto, Haruko
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Saitoh, Hiromasa
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Takahashi, Hideyuki
e07005b6-0c7f-4ede-abf4-12bf7bcf963e
Fujisaki, Koki
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Oikawa, Kaori
3f7b307a-a218-45d4-b10e-bd499657d652
Uemura, Aiko
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Natsume, Satoshi
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Jikumaru, Yusuke
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Matsuura, Hideyuki
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Umemura, Kenji
bb5ed90b-67f4-4bf3-a2eb-ccec00c755ea
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Terauchi, Ryohei
934926b5-7016-4152-aded-1a002da1f66b

Tamiru, Muluneh, Takagi, Hiroki, Abe, Akira, Yokota, Takao, Kanzaki, Hiroyuki, Okamoto, Haruko, Saitoh, Hiromasa, Takahashi, Hideyuki, Fujisaki, Koki, Oikawa, Kaori, Uemura, Aiko, Natsume, Satoshi, Jikumaru, Yusuke, Matsuura, Hideyuki, Umemura, Kenji, Terry, Matthew J. and Terauchi, Ryohei (2016) A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice. New Phytologist, 210, 1282-1297. (doi:10.1111/nph.13864).

Record type: Article

Abstract

Understanding how plants allocate their resources to growth or defence is of long-term importance to the development of new and improved varieties of different crops.
Using molecular genetics, plant physiology, hormone analysis and Next-Generation Sequencing (NGS)-based transcript profiling, we have isolated and characterized the rice(Oryza sativa) LESION AND LAMINA BENDING (LLB) gene that encodes a chloroplast-targeted putative leucine carboxyl methyltransferase.

Loss of LLB function results in reduced growth and yield, hypersensitive response (HR)-like lesions, accumulation of the antimicrobial compounds momilactones and phytocassanes, and constitutive expression of pathogenesis-related genes. Consistent with these defence-associated responses, llb shows enhanced resistance to rice blast (Magnaporthe oryzae) and bacterial blight (Xanthomonas oryzae pv. oryzae). The lesion and resistance phenotypes are likely to be caused by the over-accumulation of jasmonates (JAs) in the llb mutant including the JA precursor 12-oxo-phytodienoic acid. Additionally, llb shows an increased lamina inclination and enhanced early seedling growth due to elevated brassinosteroid (BR) synthesis and/or signalling.

These findings show that LLB functions in the chloroplast to either directly or indirectly repress both JA- and BR-mediated responses, revealing a possible mechanism for controlling how plants allocate resources for defence and growth.

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More information

Accepted/In Press date: 11 December 2015
e-pub ahead of print date: 10 February 2016
Published date: 10 February 2016
Organisations: Environmental

Identifiers

Local EPrints ID: 390315
URI: http://eprints.soton.ac.uk/id/eprint/390315
ISSN: 0028-646X
PURE UUID: 495e3da3-a5cf-4f28-985c-efa5fba05aae
ORCID for Matthew J. Terry: ORCID iD orcid.org/0000-0001-5002-2708

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Date deposited: 23 Mar 2016 12:21
Last modified: 09 Jan 2022 02:50

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Contributors

Author: Muluneh Tamiru
Author: Hiroki Takagi
Author: Akira Abe
Author: Takao Yokota
Author: Hiroyuki Kanzaki
Author: Haruko Okamoto
Author: Hiromasa Saitoh
Author: Hideyuki Takahashi
Author: Koki Fujisaki
Author: Kaori Oikawa
Author: Aiko Uemura
Author: Satoshi Natsume
Author: Yusuke Jikumaru
Author: Hideyuki Matsuura
Author: Kenji Umemura
Author: Ryohei Terauchi

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