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Post-translational control of protein function with light using a LOV-intein fusion protein

Post-translational control of protein function with light using a LOV-intein fusion protein
Post-translational control of protein function with light using a LOV-intein fusion protein
Methods for the post-translational control of protein function with light hold much value as tools in cell biology. To this end, we report a fusion protein that consists of DnaE split-inteins, flanking the light sensitive LOV2 domain of Avena sativa. The resulting chimera combines the activities of these two unrelated proteins to enable controlled formation of a functional protein via upregulation of intein splicing with blue light in bacterial and human cells.
1742-2051
1388-1393
Jones, Dale
91e7e9c4-ee15-4cb6-bafc-6c81b519ecd3
Mistry, Ishna
70e94cae-a95e-44f8-b1f3-3218d6d278cf
Tavassoli, Ali
d561cf8f-2669-46b5-b6e1-2016c85d63b2
Jones, Dale
91e7e9c4-ee15-4cb6-bafc-6c81b519ecd3
Mistry, Ishna
70e94cae-a95e-44f8-b1f3-3218d6d278cf
Tavassoli, Ali
d561cf8f-2669-46b5-b6e1-2016c85d63b2

Jones, Dale, Mistry, Ishna and Tavassoli, Ali (2016) Post-translational control of protein function with light using a LOV-intein fusion protein. Molecular BioSystems, 12 (4), 1388-1393. (doi:10.1039/C6MB00007J). (PMID:26940144)

Record type: Article

Abstract

Methods for the post-translational control of protein function with light hold much value as tools in cell biology. To this end, we report a fusion protein that consists of DnaE split-inteins, flanking the light sensitive LOV2 domain of Avena sativa. The resulting chimera combines the activities of these two unrelated proteins to enable controlled formation of a functional protein via upregulation of intein splicing with blue light in bacterial and human cells.

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Tavassoli Manuscript LOV.pdf - Accepted Manuscript
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More information

Accepted/In Press date: 1 March 2016
e-pub ahead of print date: 1 March 2016

Identifiers

Local EPrints ID: 390339
URI: http://eprints.soton.ac.uk/id/eprint/390339
DOI: doi:10.1039/C6MB00007J
ISSN: 1742-2051
PURE UUID: 6fb5d6c0-e23a-48ac-af6c-78f0d9c86be0
ORCID for Ali Tavassoli: ORCID iD orcid.org/0000-0002-7420-5063

Catalogue record

Date deposited: 24 Mar 2016 11:47
Last modified: 15 Mar 2024 05:26

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Contributors

Author: Dale Jones
Author: Ishna Mistry
Author: Ali Tavassoli ORCID iD

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