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Human vastus lateralis skeletal muscle biopsy using the Well-Blakesley Conchotome

Human vastus lateralis skeletal muscle biopsy using the Well-Blakesley Conchotome
Human vastus lateralis skeletal muscle biopsy using the Well-Blakesley Conchotome
Percutaneous muscle biopsy using the Weil-Blakesley conchotome is well established in both clinical and research practice. It is a safe, effective and well tolerated technique. The Weil-Blakesley conchotome has a sharp biting tip with a 4 - 6 mm wide hollow. It is inserted through a 5 - 10 mm skin incision and can be maneuvered for controlled tissue penetration. The tip is opened and closed within the tissue and then rotated through 90 -180° to cut the muscle. The amount of muscle obtained following repeated sampling can vary from 20 mg to 290 mg which can be processed for both histology and molecular studies. The wound needs to be kept dry and vigorous physical activity kept to a minimum for approximately 72 hr although normal levels of activity can restart immediately following the procedure. This procedure is safe and effective when close attention is paid to the selection of subjects, full asepsis and post procedure care. Both right and left vastus lateralis are suitable for biopsy dependent on participant preference.
1940-087X
1-12
Baczynska, Alicja
54390ea5-a6f8-425d-9265-03194c9869ee
Shaw, Sarah
9629b12a-8ee2-4483-a9ca-6efb4eef74c8
Roberts, Helen
5ea688b1-ef7a-4173-9da0-26290e18f253
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Aihie Sayer, Avan
fb4c2053-6d51-4fc1-9489-c3cb431b0ffb
Patel, Harnish
a1b095f6-0fe3-427a-b6fb-6247bd63f39c
Baczynska, Alicja
54390ea5-a6f8-425d-9265-03194c9869ee
Shaw, Sarah
9629b12a-8ee2-4483-a9ca-6efb4eef74c8
Roberts, Helen
5ea688b1-ef7a-4173-9da0-26290e18f253
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Aihie Sayer, Avan
fb4c2053-6d51-4fc1-9489-c3cb431b0ffb
Patel, Harnish
a1b095f6-0fe3-427a-b6fb-6247bd63f39c

Baczynska, Alicja, Shaw, Sarah, Roberts, Helen, Cooper, Cyrus, Aihie Sayer, Avan and Patel, Harnish (2016) Human vastus lateralis skeletal muscle biopsy using the Well-Blakesley Conchotome. Journal of Visualized Experiments, 1-12. (doi:10.3791/53075). (PMID:26967381)

Record type: Article

Abstract

Percutaneous muscle biopsy using the Weil-Blakesley conchotome is well established in both clinical and research practice. It is a safe, effective and well tolerated technique. The Weil-Blakesley conchotome has a sharp biting tip with a 4 - 6 mm wide hollow. It is inserted through a 5 - 10 mm skin incision and can be maneuvered for controlled tissue penetration. The tip is opened and closed within the tissue and then rotated through 90 -180° to cut the muscle. The amount of muscle obtained following repeated sampling can vary from 20 mg to 290 mg which can be processed for both histology and molecular studies. The wound needs to be kept dry and vigorous physical activity kept to a minimum for approximately 72 hr although normal levels of activity can restart immediately following the procedure. This procedure is safe and effective when close attention is paid to the selection of subjects, full asepsis and post procedure care. Both right and left vastus lateralis are suitable for biopsy dependent on participant preference.

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e-pub ahead of print date: 4 March 2016
Published date: 4 March 2016
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 390746
URI: https://eprints.soton.ac.uk/id/eprint/390746
ISSN: 1940-087X
PURE UUID: 36e2af85-2f93-47d3-84ea-c4fc8de69284
ORCID for Helen Roberts: ORCID iD orcid.org/0000-0002-5291-1880
ORCID for Cyrus Cooper: ORCID iD orcid.org/0000-0003-3510-0709

Catalogue record

Date deposited: 07 Apr 2016 08:42
Last modified: 18 Jul 2019 13:35

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Contributors

Author: Alicja Baczynska
Author: Sarah Shaw
Author: Helen Roberts ORCID iD
Author: Cyrus Cooper ORCID iD
Author: Harnish Patel

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