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Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry.

Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry.
Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry.
Skeletal stem cells (SSCs) are a sub-population of mesenchymal stromal cells (MSCs) present in bone marrow with multipotent differentiation potential. A current unmet challenge hampering their clinical translation remains the isolation of homogeneous populations of SSCs, in vitro, with consistent regeneration and differentiation capacities. Cell stiffness has been shown to play an important role in cell separation using microfluidic techniques such as inertial focusing or deterministic lateral displacement. Here we report that the mechanical properties of SSCs, and of a surrogate human osteosarcoma cell line (MG-63), differ significantly from other cell populations found in the bone marrow. Using real-time deformability cytometry, a recently introduced method for cell mechanical characterization, we demonstrate that both MG-63 and SSCs are stiffer than the three primary leukocyte lineages (lymphocytes, monocytes and granulocytes) and also stiffer than HL-60, a human leukemic progenitor cell line. In addition, we show that SSCs form a mechanically distinct sub-population of MSCs. These results represent an important step towards finding the bio-physical fingerprint of human SSCs that will allow their label-free separation from bone marrow with significant physiological and therapeutic implications.
616-623
Xavier, Miguel
23a18e02-6cee-42ff-8ee5-33a99fe5c565
Rosendahl, Philipp
236b9590-5bc7-4b8d-b5c6-605d00957bf3
Herbig, Maik
babcd615-9c8f-4d13-a1cb-96e932526c3f
Kräter, Martin
c21bbc17-c1e1-4646-8664-5263721ef178
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Bornhäuser, Martin
a748aeeb-bf11-44ba-873e-24b81ad1008c
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Guck, Jochen
9791e923-26dc-4c8a-9835-86d7e11551bc
Otto, Oliver
3134b19d-51d4-46a4-9476-a57f9876a068
Xavier, Miguel
23a18e02-6cee-42ff-8ee5-33a99fe5c565
Rosendahl, Philipp
236b9590-5bc7-4b8d-b5c6-605d00957bf3
Herbig, Maik
babcd615-9c8f-4d13-a1cb-96e932526c3f
Kräter, Martin
c21bbc17-c1e1-4646-8664-5263721ef178
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Bornhäuser, Martin
a748aeeb-bf11-44ba-873e-24b81ad1008c
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Guck, Jochen
9791e923-26dc-4c8a-9835-86d7e11551bc
Otto, Oliver
3134b19d-51d4-46a4-9476-a57f9876a068

Xavier, Miguel, Rosendahl, Philipp, Herbig, Maik, Kräter, Martin, Spencer, Daniel, Bornhäuser, Martin, Oreffo, Richard O.C., Morgan, Hywel, Guck, Jochen and Otto, Oliver (2016) Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry. Integrative Biology, 8, 616-623. (doi:10.1039/c5ib00304k). (PMID:26980074)

Record type: Article

Abstract

Skeletal stem cells (SSCs) are a sub-population of mesenchymal stromal cells (MSCs) present in bone marrow with multipotent differentiation potential. A current unmet challenge hampering their clinical translation remains the isolation of homogeneous populations of SSCs, in vitro, with consistent regeneration and differentiation capacities. Cell stiffness has been shown to play an important role in cell separation using microfluidic techniques such as inertial focusing or deterministic lateral displacement. Here we report that the mechanical properties of SSCs, and of a surrogate human osteosarcoma cell line (MG-63), differ significantly from other cell populations found in the bone marrow. Using real-time deformability cytometry, a recently introduced method for cell mechanical characterization, we demonstrate that both MG-63 and SSCs are stiffer than the three primary leukocyte lineages (lymphocytes, monocytes and granulocytes) and also stiffer than HL-60, a human leukemic progenitor cell line. In addition, we show that SSCs form a mechanically distinct sub-population of MSCs. These results represent an important step towards finding the bio-physical fingerprint of human SSCs that will allow their label-free separation from bone marrow with significant physiological and therapeutic implications.

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Accepted/In Press date: 7 March 2016
Published date: 8 March 2016
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 390755
URI: https://eprints.soton.ac.uk/id/eprint/390755
PURE UUID: a052904e-9a35-4b95-ad1f-2e6c04e674a7
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

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Date deposited: 07 Apr 2016 09:11
Last modified: 06 Jun 2018 12:53

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