Comparative genomics of carriage and disease isolates of Streptococcus pneumoniae serotype 22F reveals lineage specific divergence and niche adaptation
Comparative genomics of carriage and disease isolates of Streptococcus pneumoniae serotype 22F reveals lineage specific divergence and niche adaptation
Streptococcus pneumoniaeis a major cause of meningitis, sepsis and pneumonia worldwide. Pneumococcal conjugate vaccines (PCV) have been part of the UK's childhood immunisation programme since 2006 and have significantly reduced the incidence of disease due to vaccine efficacy in reducing carriage in the population. Here we isolated two clones of 22F (an emerging serotype of clinical concern, multilocus sequence types (MLST) 433 and 698) and conducted comparative genomic analysis on four isolates, paired by ST with one of each pair being derived from carriage and the other disease (sepsis). The most compelling observation was of non-synonymous mutations inpgdA, encoding peptidoglycanN-acetylglucosamine deacetylase A, which were found in the carriage isolates of both ST433 and 698. Deacetylation of pneumococcal peptidoglycan is known to enable resistance to lysozyme upon invasion. Whilst no other clear genotypic signatures related to disease or carriage could be determined, additional intriguing comparisons between the two STs were possible. These include the presence of an intact prophage, in addition to numerous additional phage insertions, within the carriage isolate of ST433. Contrasting gene repertoires related to virulence and colonisation, including: bacteriocins, lantibiotics, and toxin-antitoxin systems, were also observed.
1243-1251
Cleary, D.W.
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Devine, V.T.
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Jefferies, J.M.C.
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Webb, J.S.
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Bentley, S.D.
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Gladstone, R.A.
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Faust, S.N.
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Clarke, S.C.
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Cleary, D.W.
f4079c6d-d54b-4108-b346-b0069035bec0
Devine, V.T.
ce4845c0-7013-4e4f-9f57-e1616cc96e98
Jefferies, J.M.C.
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Webb, J.S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Bentley, S.D.
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Gladstone, R.A.
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Faust, S.N.
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Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Cleary, D.W., Devine, V.T., Jefferies, J.M.C., Webb, J.S., Bentley, S.D., Gladstone, R.A., Faust, S.N. and Clarke, S.C.
(2016)
Comparative genomics of carriage and disease isolates of Streptococcus pneumoniae serotype 22F reveals lineage specific divergence and niche adaptation.
Genome Biology and Evolution, 8 (4), .
(doi:10.1093/gbe/evw066).
(PMID:27016484)
Abstract
Streptococcus pneumoniaeis a major cause of meningitis, sepsis and pneumonia worldwide. Pneumococcal conjugate vaccines (PCV) have been part of the UK's childhood immunisation programme since 2006 and have significantly reduced the incidence of disease due to vaccine efficacy in reducing carriage in the population. Here we isolated two clones of 22F (an emerging serotype of clinical concern, multilocus sequence types (MLST) 433 and 698) and conducted comparative genomic analysis on four isolates, paired by ST with one of each pair being derived from carriage and the other disease (sepsis). The most compelling observation was of non-synonymous mutations inpgdA, encoding peptidoglycanN-acetylglucosamine deacetylase A, which were found in the carriage isolates of both ST433 and 698. Deacetylation of pneumococcal peptidoglycan is known to enable resistance to lysozyme upon invasion. Whilst no other clear genotypic signatures related to disease or carriage could be determined, additional intriguing comparisons between the two STs were possible. These include the presence of an intact prophage, in addition to numerous additional phage insertions, within the carriage isolate of ST433. Contrasting gene repertoires related to virulence and colonisation, including: bacteriocins, lantibiotics, and toxin-antitoxin systems, were also observed.
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Accepted/In Press date: 11 March 2016
e-pub ahead of print date: 24 March 2016
Organisations:
Clinical & Experimental Sciences
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Local EPrints ID: 391261
URI: http://eprints.soton.ac.uk/id/eprint/391261
ISSN: 1759-6653
PURE UUID: 71342a17-92c8-48f8-b298-3cbf3e986e8b
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Date deposited: 11 Apr 2016 09:39
Last modified: 15 Mar 2024 03:50
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Author:
V.T. Devine
Author:
J.M.C. Jefferies
Author:
S.D. Bentley
Author:
R.A. Gladstone
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