Characterisation of oxygen permeation into a microfluidic device for cell culture by in-situ NMR spectroscopy

Yilmaz, Ali and Utz, Marcel (2016) Characterisation of oxygen permeation into a microfluidic device for cell culture by in-situ NMR spectroscopy. Lab on a Chip, 16 (11), 2079-2085. (doi:10.1039/C6LC00396F).

Record type: Article

Abstract

A compact microfludic device for perfusion culture of mammalian cells under in-situ metabolomic observation by NMR spectroscopy is presented. The chip is made from poly(methylmethacrylate) (PMMA), and uses a poly(dimethyl siloxane) (PDMS) membrane to allow gas exchange. It is integrated with a generic micro-NMR detector developed recently in our group [J. Magn. Reson. 262, 73-80 2016]. While PMMA is an excellent material in the context of NMR, PDMS is known to produce strong background signals. To mitigate this, the device keeps the PDMS away from the detection area. The oxygen permeation into the device is quantified using a flow chemistry approach. A solution of glucose is mixed on the chip with one of glucose oxidase, before flowing through the gas exchanger. The resulting concentration of gluconate is measured by 1H NMR spectroscopy as a function of flow rate. An oxygen equilibration rate constant of 2.4 s -1 is found for the device, easily sufficient to maintain normoxic conditions in a cell culture at modest perfusion flow rates.

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