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Establishment of centromeric chromatin by the CENP-A assembly factor CAL1 requires FACT-mediated transcription

Establishment of centromeric chromatin by the CENP-A assembly factor CAL1 requires FACT-mediated transcription
Establishment of centromeric chromatin by the CENP-A assembly factor CAL1 requires FACT-mediated transcription
Centromeres are essential chromosomal structures that mediate accurate chromosome segregation during cell division. Centromeres are specified epigenetically by the heritable incorporation of the centromeric histone H3 variant CENP-A. While many of the primary factors that mediate centromeric deposition of CENP-A are known, the chromatin and DNA requirements of this process have remained elusive. Here, we uncover a role for transcription in Drosophila CENP-A deposition. Using an inducible ectopic centromere system that uncouples CENP-A deposition from endogenous centromere function and cell-cycle progression, we demonstrate that CENP-A assembly by its loading factor, CAL1, requires RNAPII-mediated transcription of the underlying DNA. This transcription depends on the CAL1 binding partner FACT, but not on CENP-A incorporation. Our work establishes RNAPII passage as a key step in chaperone-mediated CENP-A chromatin establishment and propagation.
1534-5807
73-84
Chen, Chin-Chi
a33cac9b-095c-48b4-a88a-e9488dc8bc72
Bowers, Sarion
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Lipinszki, Zoltan
e405fd6d-1bee-4725-96b7-7c315f2826ed
Palladino, Jason
c1a14c49-61d3-46a2-a820-aaf66a46f368
Trusiak, Sarah
6bea2998-77c3-457e-86ef-15b28c1c1a3d
Bettini, Emily
686264d3-dd13-4f79-ae5f-964c8576d134
Rosin, Leah
76e33708-f56c-4bb8-9951-82aa1987a484
Przewloka, Marcin
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Glover, David M.
cca9cd19-3e1e-4906-b418-41876c1f9c61
O'Neill, Rachel J.
39954429-f671-4674-b1dc-9ce555e8b19d
Mellone, Barbara G.
e60a0a72-92e1-4a6e-b480-daa38ac8f3c7
Chen, Chin-Chi
a33cac9b-095c-48b4-a88a-e9488dc8bc72
Bowers, Sarion
8d659ec3-2b00-4841-9a2d-3ef1b4fe03fa
Lipinszki, Zoltan
e405fd6d-1bee-4725-96b7-7c315f2826ed
Palladino, Jason
c1a14c49-61d3-46a2-a820-aaf66a46f368
Trusiak, Sarah
6bea2998-77c3-457e-86ef-15b28c1c1a3d
Bettini, Emily
686264d3-dd13-4f79-ae5f-964c8576d134
Rosin, Leah
76e33708-f56c-4bb8-9951-82aa1987a484
Przewloka, Marcin
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Glover, David M.
cca9cd19-3e1e-4906-b418-41876c1f9c61
O'Neill, Rachel J.
39954429-f671-4674-b1dc-9ce555e8b19d
Mellone, Barbara G.
e60a0a72-92e1-4a6e-b480-daa38ac8f3c7

Chen, Chin-Chi, Bowers, Sarion, Lipinszki, Zoltan, Palladino, Jason, Trusiak, Sarah, Bettini, Emily, Rosin, Leah, Przewloka, Marcin, Glover, David M., O'Neill, Rachel J. and Mellone, Barbara G. (2015) Establishment of centromeric chromatin by the CENP-A assembly factor CAL1 requires FACT-mediated transcription. Developmental Cell, 34 (1), 73-84. (doi:10.1016/j.devcel.2015.05.012). (PMID:26151904)

Record type: Article

Abstract

Centromeres are essential chromosomal structures that mediate accurate chromosome segregation during cell division. Centromeres are specified epigenetically by the heritable incorporation of the centromeric histone H3 variant CENP-A. While many of the primary factors that mediate centromeric deposition of CENP-A are known, the chromatin and DNA requirements of this process have remained elusive. Here, we uncover a role for transcription in Drosophila CENP-A deposition. Using an inducible ectopic centromere system that uncouples CENP-A deposition from endogenous centromere function and cell-cycle progression, we demonstrate that CENP-A assembly by its loading factor, CAL1, requires RNAPII-mediated transcription of the underlying DNA. This transcription depends on the CAL1 binding partner FACT, but not on CENP-A incorporation. Our work establishes RNAPII passage as a key step in chaperone-mediated CENP-A chromatin establishment and propagation.

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Accepted/In Press date: 18 May 2015
Published date: 6 July 2015
Organisations: Molecular and Cellular, Centre for Biological Sciences

Identifiers

Local EPrints ID: 393855
URI: https://eprints.soton.ac.uk/id/eprint/393855
ISSN: 1534-5807
PURE UUID: d99b7fd6-0305-4617-9daa-c1f6c40cfbfe
ORCID for Marcin Przewloka: ORCID iD orcid.org/0000-0002-0329-9162

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Date deposited: 09 May 2016 09:07
Last modified: 06 Jun 2018 12:19

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