The pentameric nucleoplasmin fold is present in Drosophila FKBP39 and a large number of chromatin-related proteins
The pentameric nucleoplasmin fold is present in Drosophila FKBP39 and a large number of chromatin-related proteins
Nucleoplasmin is a histone chaperone that consists of a pentameric N-terminal domain and an unstructured C-terminal tail. The pentameric core domain, a doughnut-like structure with a central pore, is only found in the nucleoplasmin family. Here, we report the first structure of a nucleoplasmin-like domain (NPL) from the unrelated Drosophila protein, FKBP39, and we present evidence that this protein associates with chromatin. Furthermore, we show that two other chromatin proteins, Arabidopsis thaliana histone deacetylase type 2 (HD2) and Saccharomyces cerevisiae Fpr4, share the NPL fold and form pentamers, or a dimer of pentamers in the case of HD2. Thus, we propose a new family of proteins that share the pentameric nucleoplasmin-like NPL domain and are found in protists, fungi, plants and animals.
1949-1963
Edlich-Muth, Christian
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Artero, Jean-Baptiste
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Callow, Phil
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Przewloka, Marcin
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Watson, Aleksandra A.
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Zhang, Wei
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Glover, David M
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Debski, Janusz
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Dadlez, Michal
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Round, Adam R.
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Forsyth, V. Trevor
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Laue, Ernest D.
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22 May 2015
Edlich-Muth, Christian
8a997f7f-677e-4569-b709-8b481930bd8a
Artero, Jean-Baptiste
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Callow, Phil
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Przewloka, Marcin
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Watson, Aleksandra A.
1e375e2f-94d4-4125-af78-d1417f60046a
Zhang, Wei
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Glover, David M
43fa3614-df56-498d-b3cc-360c129ea761
Debski, Janusz
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Dadlez, Michal
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Round, Adam R.
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Forsyth, V. Trevor
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Laue, Ernest D.
9bfea318-d6a1-493a-9055-41a9f9e06f17
Edlich-Muth, Christian, Artero, Jean-Baptiste, Callow, Phil, Przewloka, Marcin, Watson, Aleksandra A., Zhang, Wei, Glover, David M, Debski, Janusz, Dadlez, Michal, Round, Adam R., Forsyth, V. Trevor and Laue, Ernest D.
(2015)
The pentameric nucleoplasmin fold is present in Drosophila FKBP39 and a large number of chromatin-related proteins.
Journal of Molecular Biology, 427 (10), .
(doi:10.1016/j.jmb.2015.03.010).
(PMID:25813344)
Abstract
Nucleoplasmin is a histone chaperone that consists of a pentameric N-terminal domain and an unstructured C-terminal tail. The pentameric core domain, a doughnut-like structure with a central pore, is only found in the nucleoplasmin family. Here, we report the first structure of a nucleoplasmin-like domain (NPL) from the unrelated Drosophila protein, FKBP39, and we present evidence that this protein associates with chromatin. Furthermore, we show that two other chromatin proteins, Arabidopsis thaliana histone deacetylase type 2 (HD2) and Saccharomyces cerevisiae Fpr4, share the NPL fold and form pentamers, or a dimer of pentamers in the case of HD2. Thus, we propose a new family of proteins that share the pentameric nucleoplasmin-like NPL domain and are found in protists, fungi, plants and animals.
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Accepted/In Press date: 17 March 2015
Published date: 22 May 2015
Organisations:
Molecular and Cellular, Centre for Biological Sciences
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Local EPrints ID: 393856
URI: http://eprints.soton.ac.uk/id/eprint/393856
ISSN: 0022-2836
PURE UUID: 998c977e-c367-48c3-af29-af01b242ef95
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Date deposited: 09 May 2016 09:11
Last modified: 15 Mar 2024 03:54
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Contributors
Author:
Christian Edlich-Muth
Author:
Jean-Baptiste Artero
Author:
Phil Callow
Author:
Aleksandra A. Watson
Author:
Wei Zhang
Author:
David M Glover
Author:
Janusz Debski
Author:
Michal Dadlez
Author:
Adam R. Round
Author:
V. Trevor Forsyth
Author:
Ernest D. Laue
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