Insight into the architecture of the NuRD complex: structure of the RbAp48-MTA1 subcomplex
Insight into the architecture of the NuRD complex: structure of the RbAp48-MTA1 subcomplex
The nucleosome remodeling and deacetylase (NuRD) complex is a widely conserved transcriptional co-regulator that harbors both nucleosome remodeling and histone deacetylase activities. It plays a critical role in the early stages of ES cell differentiation and the reprogramming of somatic to induced pluripotent stem cells. Abnormalities in several NuRD proteins are associated with cancer and aging. We have investigated the architecture of NuRD by determining the structure of a subcomplex comprising RbAp48 and MTA1. Surprisingly, RbAp48 recognizes MTA1 using the same site that it uses to bind histone H4, showing that assembly into NuRD modulates RbAp46/48 interactions with histones. Taken together with other results, our data show that the MTA proteins act as scaffolds for NuRD complex assembly. We further show that the RbAp48-MTA1 interaction is essential for the in vivo integration of RbAp46/48 into the NuRD complex.
21844-21855
Alqarni, Saad S.M.
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Murthy, Andal
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Zhang, Wei
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Przewloka, Marcin
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Silva, Ana P.G.
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Watson, Aleksandra A.
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Lejon, Sara
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Pei, Xue Y.
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Smits, Arne H.
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Kloet, Susan L.
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Wang, Hongxin
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Shepherd, Nicholas E.
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Stokes, Philippa H.
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Blobel, Gerd A.
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Vermeulen, Michiel
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Glover, David M.
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Mackay, Joel P.
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Laue, Ernest D.
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8 August 2014
Alqarni, Saad S.M.
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Murthy, Andal
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Zhang, Wei
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Przewloka, Marcin
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Silva, Ana P.G.
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Watson, Aleksandra A.
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Lejon, Sara
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Pei, Xue Y.
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Smits, Arne H.
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Kloet, Susan L.
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Wang, Hongxin
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Shepherd, Nicholas E.
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Stokes, Philippa H.
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Blobel, Gerd A.
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Vermeulen, Michiel
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Glover, David M.
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Mackay, Joel P.
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Laue, Ernest D.
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Alqarni, Saad S.M., Murthy, Andal, Zhang, Wei, Przewloka, Marcin, Silva, Ana P.G., Watson, Aleksandra A., Lejon, Sara, Pei, Xue Y., Smits, Arne H., Kloet, Susan L., Wang, Hongxin, Shepherd, Nicholas E., Stokes, Philippa H., Blobel, Gerd A., Vermeulen, Michiel, Glover, David M., Mackay, Joel P. and Laue, Ernest D.
(2014)
Insight into the architecture of the NuRD complex: structure of the RbAp48-MTA1 subcomplex.
The Journal of Biological Chemistry, 289 (32), .
(doi:10.1074/jbc.M114.558940).
(PMID:24920672)
Abstract
The nucleosome remodeling and deacetylase (NuRD) complex is a widely conserved transcriptional co-regulator that harbors both nucleosome remodeling and histone deacetylase activities. It plays a critical role in the early stages of ES cell differentiation and the reprogramming of somatic to induced pluripotent stem cells. Abnormalities in several NuRD proteins are associated with cancer and aging. We have investigated the architecture of NuRD by determining the structure of a subcomplex comprising RbAp48 and MTA1. Surprisingly, RbAp48 recognizes MTA1 using the same site that it uses to bind histone H4, showing that assembly into NuRD modulates RbAp46/48 interactions with histones. Taken together with other results, our data show that the MTA proteins act as scaffolds for NuRD complex assembly. We further show that the RbAp48-MTA1 interaction is essential for the in vivo integration of RbAp46/48 into the NuRD complex.
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e-pub ahead of print date: 11 June 2014
Published date: 8 August 2014
Organisations:
Molecular and Cellular, Centre for Biological Sciences
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Local EPrints ID: 393858
URI: http://eprints.soton.ac.uk/id/eprint/393858
ISSN: 0021-9258
PURE UUID: 32974b1b-b480-4b61-a92c-6c12221c6f4c
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Date deposited: 09 May 2016 09:22
Last modified: 15 Mar 2024 03:54
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Contributors
Author:
Saad S.M. Alqarni
Author:
Andal Murthy
Author:
Wei Zhang
Author:
Ana P.G. Silva
Author:
Aleksandra A. Watson
Author:
Sara Lejon
Author:
Xue Y. Pei
Author:
Arne H. Smits
Author:
Susan L. Kloet
Author:
Hongxin Wang
Author:
Nicholas E. Shepherd
Author:
Philippa H. Stokes
Author:
Gerd A. Blobel
Author:
Michiel Vermeulen
Author:
David M. Glover
Author:
Joel P. Mackay
Author:
Ernest D. Laue
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