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CENP-C is a structural platform for kinetochore assembly

CENP-C is a structural platform for kinetochore assembly
CENP-C is a structural platform for kinetochore assembly
Centromeres provide a region of chromatin upon which kinetochores are assembled in mitosis. Centromeric protein C (CENP-C) is a core component of this centromeric chromatin that, when depleted, prevents the proper formation of both centromeres and kinetochores. CENP-C localizes to centromeres throughout the cell cycle via its C-terminal part, whereas its N-terminal part appears necessary for recruitment of some but not all components of the Mis12 complex of the kinetochore. We now find that all kinetochore proteins belonging to the KMN (KNL1/Spc105, the Mis12 complex, and the Ndc80 complex) network bind to the N-terminal part of Drosophila CENP-C. Moreover, we show that the Mis12 complex component Nnf1 interacts directly with CENP-C in vitro. To test whether CENP-C's N-terminal part was sufficient to recruit KMN proteins, we targeted it to the centrosome by fusing it to a domain of Plk4 kinase. The Mis12 and Ndc80 complexes and Spc105 protein were then all recruited to centrosomes at the expense of centromeres, leading to mitotic abnormalities typical of cells with defective kinetochores. Thus, the N-terminal part of Drosophila CENP-C is sufficient to recruit core kinetochore components and acts as the principal linkage between centromere and kinetochore during mitosis.
0960-9822
399-405
Przewloka, Marcin
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Venkei, Zsolt
0df721fd-a3e7-4360-94e2-8470bd03f487
Bolanos-Garcia, Victor M.
89e3daf2-d53f-4fbb-b457-f159e12f81a7
Debski, Janusz
f30de1a1-5593-4c9e-935b-89acbaeb2000
Dadlez, Michal
c6cc6bee-3459-4449-a2ab-36233c8a0cdc
Glover, David M.
cca9cd19-3e1e-4906-b418-41876c1f9c61
Przewloka, Marcin
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Venkei, Zsolt
0df721fd-a3e7-4360-94e2-8470bd03f487
Bolanos-Garcia, Victor M.
89e3daf2-d53f-4fbb-b457-f159e12f81a7
Debski, Janusz
f30de1a1-5593-4c9e-935b-89acbaeb2000
Dadlez, Michal
c6cc6bee-3459-4449-a2ab-36233c8a0cdc
Glover, David M.
cca9cd19-3e1e-4906-b418-41876c1f9c61

Przewloka, Marcin, Venkei, Zsolt, Bolanos-Garcia, Victor M., Debski, Janusz, Dadlez, Michal and Glover, David M. (2011) CENP-C is a structural platform for kinetochore assembly. Current Biology, 21 (5), 399-405. (doi:10.1016/j.cub.2011.02.005). (PMID:21353555)

Record type: Article

Abstract

Centromeres provide a region of chromatin upon which kinetochores are assembled in mitosis. Centromeric protein C (CENP-C) is a core component of this centromeric chromatin that, when depleted, prevents the proper formation of both centromeres and kinetochores. CENP-C localizes to centromeres throughout the cell cycle via its C-terminal part, whereas its N-terminal part appears necessary for recruitment of some but not all components of the Mis12 complex of the kinetochore. We now find that all kinetochore proteins belonging to the KMN (KNL1/Spc105, the Mis12 complex, and the Ndc80 complex) network bind to the N-terminal part of Drosophila CENP-C. Moreover, we show that the Mis12 complex component Nnf1 interacts directly with CENP-C in vitro. To test whether CENP-C's N-terminal part was sufficient to recruit KMN proteins, we targeted it to the centrosome by fusing it to a domain of Plk4 kinase. The Mis12 and Ndc80 complexes and Spc105 protein were then all recruited to centrosomes at the expense of centromeres, leading to mitotic abnormalities typical of cells with defective kinetochores. Thus, the N-terminal part of Drosophila CENP-C is sufficient to recruit core kinetochore components and acts as the principal linkage between centromere and kinetochore during mitosis.

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More information

Accepted/In Press date: 3 February 2011
e-pub ahead of print date: 25 February 2011
Published date: 8 March 2011
Organisations: Molecular and Cellular, Centre for Biological Sciences

Identifiers

Local EPrints ID: 393861
URI: http://eprints.soton.ac.uk/id/eprint/393861
ISSN: 0960-9822
PURE UUID: 66e0cffe-6cc9-4259-b149-2c4e20b6c50d
ORCID for Marcin Przewloka: ORCID iD orcid.org/0000-0002-0329-9162

Catalogue record

Date deposited: 13 May 2016 15:28
Last modified: 15 Sep 2021 02:06

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