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Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis in Drosophila spermatocytes

Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis in Drosophila spermatocytes
Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis in Drosophila spermatocytes
Cytokinesis, the final step of cell division, usually ends with the abscission of the two daughter cells. In some tissues, however, daughter cells never completely separate and remain interconnected by intercellular bridges or ring canals. In this paper, we report the identification and analysis of a novel ring canal component, Nessun Dorma (Nesd), isolated as an evolutionarily conserved partner of the centralspindlin complex, a key regulator of cytokinesis. Nesd contains a pectin lyase-like domain found in proteins that bind to polysaccharides, and we present evidence that it has high affinity for ?-galactosides in vitro. Moreover, nesd is an essential gene in Drosophila melanogaster, in which it is required for completion of cytokinesis during male meiosis and possibly in female germline cells. Our findings indicate that Nesd is a novel carbohydrate-binding protein that functions together with centralspindlin in late cytokinesis, thus highlighting the importance of glycosylation in this process.
1540-8140
1351-1365
Montembault, Emilie
d813cb92-f2b7-41d9-9bd4-29171f13c47a
Zhang, Wei
fa6d7061-bfce-48c0-879c-925302213c1a
Przewloka, Marcin
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Archambault, Vincent
3654cc6d-d3f9-4404-8ae5-9d8af8b008dc
Sevin, Emeric W.
b9eb8468-7fa9-4aaa-bf36-d4029c283ccd
Laue, Ernest D.
9bfea318-d6a1-493a-9055-41a9f9e06f17
Glover, David M.
cca9cd19-3e1e-4906-b418-41876c1f9c61
D'Avino, Pier Paolo
0e659e0c-c3a7-4344-8850-3364810b9445
Montembault, Emilie
d813cb92-f2b7-41d9-9bd4-29171f13c47a
Zhang, Wei
fa6d7061-bfce-48c0-879c-925302213c1a
Przewloka, Marcin
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Archambault, Vincent
3654cc6d-d3f9-4404-8ae5-9d8af8b008dc
Sevin, Emeric W.
b9eb8468-7fa9-4aaa-bf36-d4029c283ccd
Laue, Ernest D.
9bfea318-d6a1-493a-9055-41a9f9e06f17
Glover, David M.
cca9cd19-3e1e-4906-b418-41876c1f9c61
D'Avino, Pier Paolo
0e659e0c-c3a7-4344-8850-3364810b9445

Montembault, Emilie, Zhang, Wei, Przewloka, Marcin, Archambault, Vincent, Sevin, Emeric W., Laue, Ernest D., Glover, David M. and D'Avino, Pier Paolo (2010) Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis in Drosophila spermatocytes. The Journal of Cell Biology, 191 (7), 1351-1365. (doi:10.1083/jcb.201007060). (PMID:21187330)

Record type: Article

Abstract

Cytokinesis, the final step of cell division, usually ends with the abscission of the two daughter cells. In some tissues, however, daughter cells never completely separate and remain interconnected by intercellular bridges or ring canals. In this paper, we report the identification and analysis of a novel ring canal component, Nessun Dorma (Nesd), isolated as an evolutionarily conserved partner of the centralspindlin complex, a key regulator of cytokinesis. Nesd contains a pectin lyase-like domain found in proteins that bind to polysaccharides, and we present evidence that it has high affinity for ?-galactosides in vitro. Moreover, nesd is an essential gene in Drosophila melanogaster, in which it is required for completion of cytokinesis during male meiosis and possibly in female germline cells. Our findings indicate that Nesd is a novel carbohydrate-binding protein that functions together with centralspindlin in late cytokinesis, thus highlighting the importance of glycosylation in this process.

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Accepted/In Press date: 29 November 2010
Published date: 27 December 2010
Organisations: Molecular and Cellular, Centre for Biological Sciences

Identifiers

Local EPrints ID: 393862
URI: http://eprints.soton.ac.uk/id/eprint/393862
ISSN: 1540-8140
PURE UUID: a43c4b35-f8d8-4bac-affa-f291995dccbc
ORCID for Marcin Przewloka: ORCID iD orcid.org/0000-0002-0329-9162

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Date deposited: 13 May 2016 15:35
Last modified: 24 Sep 2019 00:32

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Contributors

Author: Emilie Montembault
Author: Wei Zhang
Author: Vincent Archambault
Author: Emeric W. Sevin
Author: Ernest D. Laue
Author: David M. Glover
Author: Pier Paolo D'Avino

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