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Histone H1 overexpressed to high level in tobacco affects certain developmental programs but has limited effect on basal cellular functions

Histone H1 overexpressed to high level in tobacco affects certain developmental programs but has limited effect on basal cellular functions
Histone H1 overexpressed to high level in tobacco affects certain developmental programs but has limited effect on basal cellular functions
Histone H1, a major structural component of chromatin fiber, is believed to act as a general repressor of transcription. To investigate in vivo the role of this protein in transcription regulation during development of a multicellular organism, we made transgenic tobacco plants that overexpress the gene for Arabidopsis histone H1. In all plants that overexpressed H1 the total H1-to-DNA ratio in chromatin increased 2.3-2.8 times compared with the physiological level. This was accompanied by 50-100% decrease of native tobacco H1. The phenotypic changes in H1-overexpressing plants ranged from mild to severe perturbations in morphological appearance and flowering. No correlation was observed between the extent of phenotypic change and the variation in the amount of overexpressed H1 or the presence or absence of the native tobacco H1. However, the severe phenotypic changes were correlated with early occurrence during plant growth of cells with abnormally heterochromatinized nuclei. Such cells occurred considerably later in plants with milder changes. Surprisingly, the ability of cells with highly heterochromatinized nuclei to fulfill basic physiological functions, including differentiation, was not markedly hampered. The results support the suggestion that chromatin structural changes dependent on H1 stoichiometry and on the profile of major H1 variants have limited regulatory effect on the activity of genes that control basal cellular functions. However, the H1-mediated chromatin changes can be of much greater importance for the regulation of genes involved in control of specific developmental programs.
0027-8424
10250-10255
Prymakowska-Bosak, M.
ae935b68-8e7d-40d1-9a9c-6206f28abcfc
Przewloka, M.R.
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Iwkiewicz, J.
023bf946-37cb-4fd1-a3cc-3533ce57da4e
Egierszdorff, S.
cd74f21c-b3b5-4e8a-8351-c2eaae1e0d51
Kuraś, M.
83b2a472-7bcb-449b-9e27-434ddd7bab4a
Chaubet, N.
296e8e65-a386-4715-b0d5-291d1e731f05
Gigot, C.
d6b348ea-89bd-4d7f-b45f-956fcd852151
Spiker, S.
a140d2fd-0957-4f8e-8947-3b325062b889
Jerzmanowski, A.
fd267f9e-942e-4f03-a56a-db15ad119bd6
Prymakowska-Bosak, M.
ae935b68-8e7d-40d1-9a9c-6206f28abcfc
Przewloka, M.R.
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Iwkiewicz, J.
023bf946-37cb-4fd1-a3cc-3533ce57da4e
Egierszdorff, S.
cd74f21c-b3b5-4e8a-8351-c2eaae1e0d51
Kuraś, M.
83b2a472-7bcb-449b-9e27-434ddd7bab4a
Chaubet, N.
296e8e65-a386-4715-b0d5-291d1e731f05
Gigot, C.
d6b348ea-89bd-4d7f-b45f-956fcd852151
Spiker, S.
a140d2fd-0957-4f8e-8947-3b325062b889
Jerzmanowski, A.
fd267f9e-942e-4f03-a56a-db15ad119bd6

Prymakowska-Bosak, M., Przewloka, M.R., Iwkiewicz, J., Egierszdorff, S., Kuraś, M., Chaubet, N., Gigot, C., Spiker, S. and Jerzmanowski, A. (1996) Histone H1 overexpressed to high level in tobacco affects certain developmental programs but has limited effect on basal cellular functions. Proceedings of the National Academy of Sciences, 93 (19), 10250-10255. (PMID:8816786)

Record type: Article

Abstract

Histone H1, a major structural component of chromatin fiber, is believed to act as a general repressor of transcription. To investigate in vivo the role of this protein in transcription regulation during development of a multicellular organism, we made transgenic tobacco plants that overexpress the gene for Arabidopsis histone H1. In all plants that overexpressed H1 the total H1-to-DNA ratio in chromatin increased 2.3-2.8 times compared with the physiological level. This was accompanied by 50-100% decrease of native tobacco H1. The phenotypic changes in H1-overexpressing plants ranged from mild to severe perturbations in morphological appearance and flowering. No correlation was observed between the extent of phenotypic change and the variation in the amount of overexpressed H1 or the presence or absence of the native tobacco H1. However, the severe phenotypic changes were correlated with early occurrence during plant growth of cells with abnormally heterochromatinized nuclei. Such cells occurred considerably later in plants with milder changes. Surprisingly, the ability of cells with highly heterochromatinized nuclei to fulfill basic physiological functions, including differentiation, was not markedly hampered. The results support the suggestion that chromatin structural changes dependent on H1 stoichiometry and on the profile of major H1 variants have limited regulatory effect on the activity of genes that control basal cellular functions. However, the H1-mediated chromatin changes can be of much greater importance for the regulation of genes involved in control of specific developmental programs.

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Published date: 17 September 1996
Organisations: Molecular and Cellular, Centre for Biological Sciences

Identifiers

Local EPrints ID: 393871
URI: https://eprints.soton.ac.uk/id/eprint/393871
ISSN: 0027-8424
PURE UUID: 83b44615-d1fb-4eec-8c11-3e6368d58527
ORCID for M.R. Przewloka: ORCID iD orcid.org/0000-0002-0329-9162

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Date deposited: 26 May 2016 11:39
Last modified: 05 Nov 2019 01:33

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Contributors

Author: M. Prymakowska-Bosak
Author: M.R. Przewloka ORCID iD
Author: J. Iwkiewicz
Author: S. Egierszdorff
Author: M. Kuraś
Author: N. Chaubet
Author: C. Gigot
Author: S. Spiker
Author: A. Jerzmanowski

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