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Electrically addressed dual resonator sensing platform for biochemical detection

Electrically addressed dual resonator sensing platform for biochemical detection
Electrically addressed dual resonator sensing platform for biochemical detection


Chemically functionalized silicon microresonators provide the potential for sensitive, label-free biomolecular detection by coupling small induced perturbations in stiffness, mass, and dissipation due to surface bound analyte to their measured frequency response. However, several implementation challenges arise from the necessity of operation in compatible biological buffer solutions. These challenges include minimizing undesired effects of fluid-structure interaction and buffer interference with signal transduction. In this paper, we present a novel dual resonator sensing platform (DRP) to address these challenges, wherein electrical transduction and biochemical sensing are spatially separated onto two different mechanically coupled resonators. This enables electrical interrogation of the sensor without compromising the sensing environment, allowing for relative ease of fabrication and the possibility of integration with on-chip electronics. We demonstrate the functionality of the DRP as a mass sensing platform, with a mass responsivity of 34 Hz/ng in air. The viscous effects on dynamic response of the DRP were investigated by comparing the measurements with theoretical values, and a quality factor of 221 in water is demonstrated. Furthermore, characterization of the DRP was preformed with streptavidin-coated microbeads, and the measured response is in close agreement with the model. Finally, the use of DRP for measurement of dried cell mass and accurate cell counting is demonstrated with a detection limit of 1.46 ng.
Published in:
1057-7157
34-43
Lin, Angel T.-H.
9a64197d-3714-449c-9720-9ef4ea5f2689
Yan, Jize
786dc090-843b-435d-adbe-1d35e8fc5828
Seshia, Ashwin A.
6fe2b5b5-e451-41e2-a23a-601c9faf7d8a
Lin, Angel T.-H.
9a64197d-3714-449c-9720-9ef4ea5f2689
Yan, Jize
786dc090-843b-435d-adbe-1d35e8fc5828
Seshia, Ashwin A.
6fe2b5b5-e451-41e2-a23a-601c9faf7d8a

Lin, Angel T.-H., Yan, Jize and Seshia, Ashwin A. (2012) Electrically addressed dual resonator sensing platform for biochemical detection. Journal of Microelectromechanical Systems, 21 (1), 34-43. (doi:10.1109/JMEMS.2011.2174420).

Record type: Article

Abstract



Chemically functionalized silicon microresonators provide the potential for sensitive, label-free biomolecular detection by coupling small induced perturbations in stiffness, mass, and dissipation due to surface bound analyte to their measured frequency response. However, several implementation challenges arise from the necessity of operation in compatible biological buffer solutions. These challenges include minimizing undesired effects of fluid-structure interaction and buffer interference with signal transduction. In this paper, we present a novel dual resonator sensing platform (DRP) to address these challenges, wherein electrical transduction and biochemical sensing are spatially separated onto two different mechanically coupled resonators. This enables electrical interrogation of the sensor without compromising the sensing environment, allowing for relative ease of fabrication and the possibility of integration with on-chip electronics. We demonstrate the functionality of the DRP as a mass sensing platform, with a mass responsivity of 34 Hz/ng in air. The viscous effects on dynamic response of the DRP were investigated by comparing the measurements with theoretical values, and a quality factor of 221 in water is demonstrated. Furthermore, characterization of the DRP was preformed with streptavidin-coated microbeads, and the measured response is in close agreement with the model. Finally, the use of DRP for measurement of dried cell mass and accurate cell counting is demonstrated with a detection limit of 1.46 ng.
Published in:

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Accepted/In Press date: 13 September 2011
e-pub ahead of print date: 1 December 2011
Published date: February 2012
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 394379
URI: https://eprints.soton.ac.uk/id/eprint/394379
ISSN: 1057-7157
PURE UUID: a98f2efd-e162-49ce-9312-ae5a30b3ee76
ORCID for Jize Yan: ORCID iD orcid.org/0000-0002-2886-2847

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Date deposited: 17 Jun 2016 15:52
Last modified: 20 Jul 2019 00:34

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Contributors

Author: Angel T.-H. Lin
Author: Jize Yan ORCID iD
Author: Ashwin A. Seshia

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