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Long-term in vivo single-cell tracking reveals the switch of migration patterns in adult-born juxtaglomerular cells of the mouse olfactory bulb

Long-term in vivo single-cell tracking reveals the switch of migration patterns in adult-born juxtaglomerular cells of the mouse olfactory bulb
Long-term in vivo single-cell tracking reveals the switch of migration patterns in adult-born juxtaglomerular cells of the mouse olfactory bulb
The behavior of adult-born cells can be easily monitored in cell culture or in lower model organisms, but longitudinal observation of individual mammalian adult-born cells in their native microenvironment still proves to be a challenge. Here we have established an approach named optical cell positioning system for long-term in vivo single-cell tracking, which integrates red-green-blue cell labeling with repeated angiography. By combining this approach with in vivo two-photon imaging technique, we characterized the in vivo migration patterns of adult-born neurons in the olfactory bulb. In contrast to the traditional view of mere radial migration of adult-born cells within the bulb, we found that juxtaglomerular cells switch from radial migration to long distance lateral migration upon arrival in their destination layer. This unique long-distance lateral migration has characteristic temporal (stop-and-go) and spatial (migratory, unidirectional or multidirectional) patterns, with a clear cell age-dependent decrease in the migration speed. The active migration of adult-born cells coincides with the time period of initial fate determination and is likely to impact on the integration sites of adult-born cells, their odor responsiveness, as well as their survival rate.
1001-0602
1-44
Liang, Yajie
9f9476f7-a4ac-4b3a-87fc-2dd83ee1af16
Li, Kaizhen
49fd8b31-e763-4b28-946f-e78fcd74fd7a
Riecken, Kristoffer
0eca316e-6c54-4cda-80e0-92c571263996
Maslyukov, Anatoliy
bfde9026-d910-4c09-97a3-7c511e21f7e9
Gomez-Nicola, Diego
0680aa66-9dee-47cf-a8d3-e39c988f85b5
Kovalchuk, Yury
f229bb84-2ebc-446d-b854-a95ca312571d
Fehse, Boris
c4936883-d98d-48fc-91f4-691ef0c7ee5a
Garaschuk, Olga
f751209b-b876-41b8-9d83-b999f3ce1f82
Liang, Yajie
9f9476f7-a4ac-4b3a-87fc-2dd83ee1af16
Li, Kaizhen
49fd8b31-e763-4b28-946f-e78fcd74fd7a
Riecken, Kristoffer
0eca316e-6c54-4cda-80e0-92c571263996
Maslyukov, Anatoliy
bfde9026-d910-4c09-97a3-7c511e21f7e9
Gomez-Nicola, Diego
0680aa66-9dee-47cf-a8d3-e39c988f85b5
Kovalchuk, Yury
f229bb84-2ebc-446d-b854-a95ca312571d
Fehse, Boris
c4936883-d98d-48fc-91f4-691ef0c7ee5a
Garaschuk, Olga
f751209b-b876-41b8-9d83-b999f3ce1f82

Liang, Yajie, Li, Kaizhen, Riecken, Kristoffer, Maslyukov, Anatoliy, Gomez-Nicola, Diego, Kovalchuk, Yury, Fehse, Boris and Garaschuk, Olga (2016) Long-term in vivo single-cell tracking reveals the switch of migration patterns in adult-born juxtaglomerular cells of the mouse olfactory bulb. Cell Research, 1-44. (doi:10.1038/cr.2016.55). (PMID:27174051)

Record type: Article

Abstract

The behavior of adult-born cells can be easily monitored in cell culture or in lower model organisms, but longitudinal observation of individual mammalian adult-born cells in their native microenvironment still proves to be a challenge. Here we have established an approach named optical cell positioning system for long-term in vivo single-cell tracking, which integrates red-green-blue cell labeling with repeated angiography. By combining this approach with in vivo two-photon imaging technique, we characterized the in vivo migration patterns of adult-born neurons in the olfactory bulb. In contrast to the traditional view of mere radial migration of adult-born cells within the bulb, we found that juxtaglomerular cells switch from radial migration to long distance lateral migration upon arrival in their destination layer. This unique long-distance lateral migration has characteristic temporal (stop-and-go) and spatial (migratory, unidirectional or multidirectional) patterns, with a clear cell age-dependent decrease in the migration speed. The active migration of adult-born cells coincides with the time period of initial fate determination and is likely to impact on the integration sites of adult-born cells, their odor responsiveness, as well as their survival rate.

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Accepted/In Press date: 5 April 2016
e-pub ahead of print date: 13 May 2016
Organisations: Biomedicine

Identifiers

Local EPrints ID: 394403
URI: https://eprints.soton.ac.uk/id/eprint/394403
ISSN: 1001-0602
PURE UUID: 1673444e-b1c2-4d71-b50d-ac0f0f46c121
ORCID for Diego Gomez-Nicola: ORCID iD orcid.org/0000-0002-5316-2682

Catalogue record

Date deposited: 18 May 2016 11:33
Last modified: 06 Jun 2018 12:33

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