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Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration

Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration
Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration
The use of skeletal stem cells (SSCs) for cell-based therapies is currently one of the most promising areas for skeletal disease treatment and skeletal tissue repair. The ability for controlled modification of SSCs could provide significant therapeutic potential in regenerative medicine, with the prospect to permanently repopulate a host with stem cells and their progeny. Currently, SSC differentiation into the stromal lineages of bone, fat and cartilage is assessed using different approaches that typically require cell fixation or lysis, which are invasive or even destructive. Raman spectroscopy and coherent anti-Stokes Raman scattering (CARS) microscopy present an exciting alternative for studying biological systems in their natural state, without any perturbation. Here we review the applications of Raman spectroscopy and CARS imaging in stem-cell research, and discuss the potential of these two techniques for evaluating SSCs, skeletal tissues and skeletal regeneration as an exemplar.
1-12
Costa Moura, Catarina
fdd85e38-f46f-47d0-a33a-6e7328398e90
Tare, Rahul
587c9db4-e409-4e7c-a02a-677547ab724a
Oreffo, Richard O.C.
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Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Costa Moura, Catarina
fdd85e38-f46f-47d0-a33a-6e7328398e90
Tare, Rahul
587c9db4-e409-4e7c-a02a-677547ab724a
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9

Costa Moura, Catarina, Tare, Rahul, Oreffo, Richard O.C. and Mahajan, Sumeet (2016) Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration. Journal of the Royal Society Interface, 13 (118), 1-12. (doi:10.1098/rsif.2016.0182). (PMID:27170652)

Record type: Article

Abstract

The use of skeletal stem cells (SSCs) for cell-based therapies is currently one of the most promising areas for skeletal disease treatment and skeletal tissue repair. The ability for controlled modification of SSCs could provide significant therapeutic potential in regenerative medicine, with the prospect to permanently repopulate a host with stem cells and their progeny. Currently, SSC differentiation into the stromal lineages of bone, fat and cartilage is assessed using different approaches that typically require cell fixation or lysis, which are invasive or even destructive. Raman spectroscopy and coherent anti-Stokes Raman scattering (CARS) microscopy present an exciting alternative for studying biological systems in their natural state, without any perturbation. Here we review the applications of Raman spectroscopy and CARS imaging in stem-cell research, and discuss the potential of these two techniques for evaluating SSCs, skeletal tissues and skeletal regeneration as an exemplar.

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Accepted/In Press date: 13 April 2016
e-pub ahead of print date: 11 May 2016
Organisations: Institute for Life Sciences, Human Development & Health, Chemical Biology Group

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Local EPrints ID: 394850
URI: http://eprints.soton.ac.uk/id/eprint/394850
PURE UUID: 97d16e31-f6b9-4afe-b92b-75b8951ea3d3
ORCID for Rahul Tare: ORCID iD orcid.org/0000-0001-8274-8837
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Sumeet Mahajan: ORCID iD orcid.org/0000-0001-8923-6666

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Date deposited: 24 May 2016 15:40
Last modified: 18 Feb 2021 17:09

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Contributors

Author: Catarina Costa Moura
Author: Rahul Tare ORCID iD
Author: Sumeet Mahajan ORCID iD

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