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Influence of inoculum to substrate ratio on the biochemical methane potential of maize in batch tests

Influence of inoculum to substrate ratio on the biochemical methane potential of maize in batch tests
Influence of inoculum to substrate ratio on the biochemical methane potential of maize in batch tests
Completely mixed batch reactors were used to compare the methane production from maize at inoculum to substrate volatile solids ratios (r(l/s)) of 3, 2, 1.5 and 1. The tests were carried out at 35 degrees C and run against a control of inoculum without substrate. The stability and progress of the reaction from solid substrate to gaseous end product was monitored by measuring the pH, soluble chemical oxygen demand (CODs), total alkalinity (TA), volatile fatty acids (VFA) and gas composition on a daily basis. The results showed that the yield coefficient showed little variation over the range used, with an average value of 211 +/- 6 ml CH4 at standard temperature and pressure (STP) conditions g VSadded.-1 The methane added production curves were further analysed to give values for the maximum specific methane production rate, ranging from 10 ml CH4 a. VSS-1 day(-1) for a r(l/s) of 3-23 ml CH4 g VSS-1 day(-1) for a r(1/s) of 1. The initial buffering capacity of the system was enhanced by addition of NaHCO3 to 9 100 mg CaCO3 1(-1) which allowed digestion to proceed within the pH range 7.2-8.0 and at TVFA to TA ratio below the critical limit of 0.4. The largest proportion of the CODs was in the form of VFA and the reactor with the highest VS loading showed an accumulation of longer chain acids.
anaerobic digestion, maize, biochemical methane potential (bmp), inoculum to substrate ratio
1359-5113
1444-1450
Raposo, F.
47104451-511a-4b03-aaa9-67348da4567d
Banks, C.J.
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f
Siegert, I.
0d4cc94d-6bd6-430c-acec-192deeb69ac3
Heaven, S.
f25f74b6-97bd-4a18-b33b-a63084718571
Borja, R.
ed513484-04ff-4424-ab79-dc715ca63146
Raposo, F.
47104451-511a-4b03-aaa9-67348da4567d
Banks, C.J.
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f
Siegert, I.
0d4cc94d-6bd6-430c-acec-192deeb69ac3
Heaven, S.
f25f74b6-97bd-4a18-b33b-a63084718571
Borja, R.
ed513484-04ff-4424-ab79-dc715ca63146

Raposo, F., Banks, C.J., Siegert, I., Heaven, S. and Borja, R. (2006) Influence of inoculum to substrate ratio on the biochemical methane potential of maize in batch tests. Process Biochemistry, 41 (6), 1444-1450. (doi:10.1016/j.procbio.2006.01.012).

Record type: Article

Abstract

Completely mixed batch reactors were used to compare the methane production from maize at inoculum to substrate volatile solids ratios (r(l/s)) of 3, 2, 1.5 and 1. The tests were carried out at 35 degrees C and run against a control of inoculum without substrate. The stability and progress of the reaction from solid substrate to gaseous end product was monitored by measuring the pH, soluble chemical oxygen demand (CODs), total alkalinity (TA), volatile fatty acids (VFA) and gas composition on a daily basis. The results showed that the yield coefficient showed little variation over the range used, with an average value of 211 +/- 6 ml CH4 at standard temperature and pressure (STP) conditions g VSadded.-1 The methane added production curves were further analysed to give values for the maximum specific methane production rate, ranging from 10 ml CH4 a. VSS-1 day(-1) for a r(l/s) of 3-23 ml CH4 g VSS-1 day(-1) for a r(1/s) of 1. The initial buffering capacity of the system was enhanced by addition of NaHCO3 to 9 100 mg CaCO3 1(-1) which allowed digestion to proceed within the pH range 7.2-8.0 and at TVFA to TA ratio below the critical limit of 0.4. The largest proportion of the CODs was in the form of VFA and the reactor with the highest VS loading showed an accumulation of longer chain acids.

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More information

Published date: June 2006
Keywords: anaerobic digestion, maize, biochemical methane potential (bmp), inoculum to substrate ratio

Identifiers

Local EPrints ID: 39501
URI: http://eprints.soton.ac.uk/id/eprint/39501
ISSN: 1359-5113
PURE UUID: 55bc7ed3-48f7-406f-b482-0ad8a9ec850e
ORCID for C.J. Banks: ORCID iD orcid.org/0000-0001-6795-814X
ORCID for S. Heaven: ORCID iD orcid.org/0000-0001-7798-4683

Catalogue record

Date deposited: 28 Jun 2006
Last modified: 16 Mar 2024 02:52

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Contributors

Author: F. Raposo
Author: C.J. Banks ORCID iD
Author: I. Siegert
Author: S. Heaven ORCID iD
Author: R. Borja

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