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Morphological convergence between an allopolyploid and one of its parental species correlates with biased gene expression and DNA loss

Morphological convergence between an allopolyploid and one of its parental species correlates with biased gene expression and DNA loss
Morphological convergence between an allopolyploid and one of its parental species correlates with biased gene expression and DNA loss
The contribution of gene expression modulation to phenotypic evolution is of major importance to an understanding of the origin of divergent or convergent phenotypes during and following polyploid speciation. Here we analysed genome-wide gene expression in two subspecies of the allotetraploid species, Senecio mohavensis A. Gray, and its diploid parents S. flavus (Decne.) Sch. Bip. and S. glaucus L.. The tetraploid is morphologically much more similar to S. flavus, leading to earlier confusion over its taxonomic status. By means of an analysis of transcriptomes of all three species, we show that gene expression divergence between the parent species is relatively low (ca. 14% of loci), whereas there is significant unequal expression between ca. 20-25% of the parental homoeologues (gene copies) in the tetraploid. The majority of the expression bias in the tetraploid is in favour of S. flavus homoeologues (ca. 65% of the differentially expressed loci), and overall expression of this parental species sub-genome is higher than that of the S. glaucus sub-genome. To determine whether absence of expression of a particular S. glaucus homoeologue in the allotetraploid could be due to loss of DNA, we carried out a PCR-based assay and confirmed that in three out of 10 loci the S. glaucus homoeologue appeared absent. Our results suggest that biased gene expression is one cause of the allotetraploid S. mohavensis being more similar in morphology to one of its parent, S. flavus, and that such bias could result, in part, from loss of S. glaucus homoeologues at some loci in the allotetraploid.
0022-1503
445-454
Alexander-Webber, Douglas
2667bdc2-daf5-41ed-8c3f-f5c36884e646
Abbott, Richard J.
d52f6834-ba40-4c9f-9aa5-9be0b4184b6b
Chapman, Mark A.
8bac4a92-bfa7-4c3c-af29-9af852ef6383
Alexander-Webber, Douglas
2667bdc2-daf5-41ed-8c3f-f5c36884e646
Abbott, Richard J.
d52f6834-ba40-4c9f-9aa5-9be0b4184b6b
Chapman, Mark A.
8bac4a92-bfa7-4c3c-af29-9af852ef6383

Alexander-Webber, Douglas, Abbott, Richard J. and Chapman, Mark A. (2016) Morphological convergence between an allopolyploid and one of its parental species correlates with biased gene expression and DNA loss. Journal of Heredity, 107 (5), 445-454. (doi:10.1093/jhered/esw035). (PMID:27217580)

Record type: Article

Abstract

The contribution of gene expression modulation to phenotypic evolution is of major importance to an understanding of the origin of divergent or convergent phenotypes during and following polyploid speciation. Here we analysed genome-wide gene expression in two subspecies of the allotetraploid species, Senecio mohavensis A. Gray, and its diploid parents S. flavus (Decne.) Sch. Bip. and S. glaucus L.. The tetraploid is morphologically much more similar to S. flavus, leading to earlier confusion over its taxonomic status. By means of an analysis of transcriptomes of all three species, we show that gene expression divergence between the parent species is relatively low (ca. 14% of loci), whereas there is significant unequal expression between ca. 20-25% of the parental homoeologues (gene copies) in the tetraploid. The majority of the expression bias in the tetraploid is in favour of S. flavus homoeologues (ca. 65% of the differentially expressed loci), and overall expression of this parental species sub-genome is higher than that of the S. glaucus sub-genome. To determine whether absence of expression of a particular S. glaucus homoeologue in the allotetraploid could be due to loss of DNA, we carried out a PCR-based assay and confirmed that in three out of 10 loci the S. glaucus homoeologue appeared absent. Our results suggest that biased gene expression is one cause of the allotetraploid S. mohavensis being more similar in morphology to one of its parent, S. flavus, and that such bias could result, in part, from loss of S. glaucus homoeologues at some loci in the allotetraploid.

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Accepted/In Press date: 20 May 2016
e-pub ahead of print date: 23 May 2016
Published date: September 2016
Organisations: Environmental

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Local EPrints ID: 395211
URI: http://eprints.soton.ac.uk/id/eprint/395211
ISSN: 0022-1503
PURE UUID: 8d494edd-595b-4669-beb9-db3e9345a824
ORCID for Mark A. Chapman: ORCID iD orcid.org/0000-0002-7151-723X

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Date deposited: 26 May 2016 08:30
Last modified: 17 Dec 2019 06:48

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Author: Douglas Alexander-Webber
Author: Richard J. Abbott
Author: Mark A. Chapman ORCID iD

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