The University of Southampton
University of Southampton Institutional Repository

Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice

Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice
Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice
Sebocytes, which are characterized by lipid accumulation that leads to cell disruption, can be found in hair follicle-associated sebaceous glands (SGs) or in free SGs such as the Meibomian glands in the eyelids. Because genetic tools that allow targeting of sebocytes while maintaining intact epidermal lipids are lacking, the relevance of sebaceous lipids in health and disease remains poorly understood. Using Scd3, which is expressed exclusively in mature sebocytes, we established a mouse line with sebocyte-specific expression of Cre recombinase. Both RT-PCR analysis and crossing into Rosa26-lacZ reporter mice and KrasG12D mice confirmed Cre activity specifically in SGs, with no activity in other skin compartments. Importantly, loss of SCD3 function did not cause detectable phenotypical alterations, endorsing the usefulness of Scd3-Cre mice for further functional studies. Scd3-Cre-induced, diphtheria chain A toxin-mediated depletion of sebaceous lipids resulted in impaired water repulsion and thermoregulation, increased rates of UVB-induced epidermal apoptosis and caused a severe pathology of the ocular surface resembling Meibomian gland dysfunction. This novel mouse line will be useful for further investigating the roles of sebaceous lipids in skin and eye integrity.
1477-9129
1823-1831
Dahlhoff, Maik
73308f3a-c3de-4f53-954b-de626f37d74e
Camera, Emanuela
d3f06f42-06ae-471d-99ce-ecead6ce3193
Schäfer, Matthias
e450f5a0-0e45-4ec8-b9fa-448e04c928f4
Emrich, Daniela
b01532e4-80c8-4175-aa8d-3657c6f9759a
Riethmacher, Dieter
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Foster, April
9fdf50f6-40b3-4506-b1f5-0ed1ad24d7f5
Paus, Ralf
aecd069e-f385-4cf2-9c40-908f2e8a7b68
Schneider, Marlon R.
8d66938a-28bc-4860-8223-163e93532a9d
Dahlhoff, Maik
73308f3a-c3de-4f53-954b-de626f37d74e
Camera, Emanuela
d3f06f42-06ae-471d-99ce-ecead6ce3193
Schäfer, Matthias
e450f5a0-0e45-4ec8-b9fa-448e04c928f4
Emrich, Daniela
b01532e4-80c8-4175-aa8d-3657c6f9759a
Riethmacher, Dieter
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Foster, April
9fdf50f6-40b3-4506-b1f5-0ed1ad24d7f5
Paus, Ralf
aecd069e-f385-4cf2-9c40-908f2e8a7b68
Schneider, Marlon R.
8d66938a-28bc-4860-8223-163e93532a9d

Dahlhoff, Maik, Camera, Emanuela, Schäfer, Matthias, Emrich, Daniela, Riethmacher, Dieter, Foster, April, Paus, Ralf and Schneider, Marlon R. (2016) Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice. Development, 143 (10), 1823-1831. (doi:10.1242/dev.132753).

Record type: Article

Abstract

Sebocytes, which are characterized by lipid accumulation that leads to cell disruption, can be found in hair follicle-associated sebaceous glands (SGs) or in free SGs such as the Meibomian glands in the eyelids. Because genetic tools that allow targeting of sebocytes while maintaining intact epidermal lipids are lacking, the relevance of sebaceous lipids in health and disease remains poorly understood. Using Scd3, which is expressed exclusively in mature sebocytes, we established a mouse line with sebocyte-specific expression of Cre recombinase. Both RT-PCR analysis and crossing into Rosa26-lacZ reporter mice and KrasG12D mice confirmed Cre activity specifically in SGs, with no activity in other skin compartments. Importantly, loss of SCD3 function did not cause detectable phenotypical alterations, endorsing the usefulness of Scd3-Cre mice for further functional studies. Scd3-Cre-induced, diphtheria chain A toxin-mediated depletion of sebaceous lipids resulted in impaired water repulsion and thermoregulation, increased rates of UVB-induced epidermal apoptosis and caused a severe pathology of the ocular surface resembling Meibomian gland dysfunction. This novel mouse line will be useful for further investigating the roles of sebaceous lipids in skin and eye integrity.

Text
1823 full.pdf - Version of Record
Download (5MB)

More information

Accepted/In Press date: 9 March 2016
e-pub ahead of print date: 17 May 2016
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 395337
URI: http://eprints.soton.ac.uk/id/eprint/395337
ISSN: 1477-9129
PURE UUID: f9a7ed80-14c5-410d-9462-22f7b2ed31f3
ORCID for Dieter Riethmacher: ORCID iD orcid.org/0000-0002-4206-5529

Catalogue record

Date deposited: 25 May 2016 13:27
Last modified: 17 Dec 2019 06:48

Export record

Altmetrics

Contributors

Author: Maik Dahlhoff
Author: Emanuela Camera
Author: Matthias Schäfer
Author: Daniela Emrich
Author: Dieter Riethmacher ORCID iD
Author: April Foster
Author: Ralf Paus
Author: Marlon R. Schneider

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×