Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice
Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice
Sebocytes, which are characterized by lipid accumulation that leads to cell disruption, can be found in hair follicle-associated sebaceous glands (SGs) or in free SGs such as the Meibomian glands in the eyelids. Because genetic tools that allow targeting of sebocytes while maintaining intact epidermal lipids are lacking, the relevance of sebaceous lipids in health and disease remains poorly understood. Using Scd3, which is expressed exclusively in mature sebocytes, we established a mouse line with sebocyte-specific expression of Cre recombinase. Both RT-PCR analysis and crossing into Rosa26-lacZ reporter mice and KrasG12D mice confirmed Cre activity specifically in SGs, with no activity in other skin compartments. Importantly, loss of SCD3 function did not cause detectable phenotypical alterations, endorsing the usefulness of Scd3-Cre mice for further functional studies. Scd3-Cre-induced, diphtheria chain A toxin-mediated depletion of sebaceous lipids resulted in impaired water repulsion and thermoregulation, increased rates of UVB-induced epidermal apoptosis and caused a severe pathology of the ocular surface resembling Meibomian gland dysfunction. This novel mouse line will be useful for further investigating the roles of sebaceous lipids in skin and eye integrity.
1823-1831
Dahlhoff, Maik
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Camera, Emanuela
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Schäfer, Matthias
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Emrich, Daniela
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Riethmacher, Dieter
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Foster, April
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Paus, Ralf
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Schneider, Marlon R.
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Dahlhoff, Maik
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Camera, Emanuela
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Schäfer, Matthias
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Emrich, Daniela
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Riethmacher, Dieter
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Foster, April
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Paus, Ralf
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Schneider, Marlon R.
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Dahlhoff, Maik, Camera, Emanuela, Schäfer, Matthias, Emrich, Daniela, Riethmacher, Dieter, Foster, April, Paus, Ralf and Schneider, Marlon R.
(2016)
Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice.
Development, 143 (10), .
(doi:10.1242/dev.132753).
Abstract
Sebocytes, which are characterized by lipid accumulation that leads to cell disruption, can be found in hair follicle-associated sebaceous glands (SGs) or in free SGs such as the Meibomian glands in the eyelids. Because genetic tools that allow targeting of sebocytes while maintaining intact epidermal lipids are lacking, the relevance of sebaceous lipids in health and disease remains poorly understood. Using Scd3, which is expressed exclusively in mature sebocytes, we established a mouse line with sebocyte-specific expression of Cre recombinase. Both RT-PCR analysis and crossing into Rosa26-lacZ reporter mice and KrasG12D mice confirmed Cre activity specifically in SGs, with no activity in other skin compartments. Importantly, loss of SCD3 function did not cause detectable phenotypical alterations, endorsing the usefulness of Scd3-Cre mice for further functional studies. Scd3-Cre-induced, diphtheria chain A toxin-mediated depletion of sebaceous lipids resulted in impaired water repulsion and thermoregulation, increased rates of UVB-induced epidermal apoptosis and caused a severe pathology of the ocular surface resembling Meibomian gland dysfunction. This novel mouse line will be useful for further investigating the roles of sebaceous lipids in skin and eye integrity.
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Accepted/In Press date: 9 March 2016
e-pub ahead of print date: 17 May 2016
Organisations:
Human Development & Health
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Local EPrints ID: 395337
URI: http://eprints.soton.ac.uk/id/eprint/395337
ISSN: 1477-9129
PURE UUID: f9a7ed80-14c5-410d-9462-22f7b2ed31f3
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Date deposited: 25 May 2016 13:27
Last modified: 15 Mar 2024 05:36
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Author:
Maik Dahlhoff
Author:
Emanuela Camera
Author:
Matthias Schäfer
Author:
Daniela Emrich
Author:
Dieter Riethmacher
Author:
April Foster
Author:
Ralf Paus
Author:
Marlon R. Schneider
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