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Hypothalamus proteomics from mouse models with obesity and anorexia reveals therapeutic targets of appetite regulation

Hypothalamus proteomics from mouse models with obesity and anorexia reveals therapeutic targets of appetite regulation
Hypothalamus proteomics from mouse models with obesity and anorexia reveals therapeutic targets of appetite regulation
Objective: This study examined the proteomic profile of the hypothalamus in mice exposed to a high-fat diet (HFD) or with the anorexia of acute illness. This comparison could provide insight on the effects of these two opposite states of energy balance on appetite regulation.

Methods: Four to six-week-old male C56BL/6J mice were fed a normal (control 1 group; n=7) or a HFD (HFD group; n=10) for 8 weeks. The control 2 (n=7) and lipopolysaccharide (LPS) groups (n=10) were fed a normal diet for 8 weeks before receiving an injection of saline and LPS, respectively. Hypothalamic regions were analysed using a quantitative proteomics method based on a combination of techniques including iTRAQ stable isotope labeling, orthogonal two-dimensional liquid chromatography hyphenated with nanospray ionization and high-resolution mass spectrometry. Key proteins were validated with quantitative PCR.

Results: Quantitative proteomics of the hypothalamous regions profiled a total of 9249 protein groups (q<0.05). Of these, 7718 protein groups were profiled with a minimum of two unique peptides for each. Hierachical clustering of the differentiated proteome revealed distinct proteomic signatures for the hypothalamus under the HFD and LPS nutritional conditions. Literature research with in silico bioinformatics interpretation of the differentiated proteome identified key biological relevant proteins and implicated pathways. Furthermore, the study identified potential pharmacologic targets. In the LPS groups, the anorexigen pro-opiomelanocortin was downregulated. In mice with obesity, nuclear factor-?B, glycine receptor subunit alpha-4 (GlyR) and neuropeptide Y levels were elevated, whereas serotonin receptor 1B levels decreased.

Conclusions: High-precision quantitative proteomics revealed that under acute systemic inflammation in the hypothalamus as a response to LPS, homeostatic mechanisms mediating loss of appetite take effect. Conversely, under chronic inflammation in the hypothalamus as a response to HFD, mechanisms mediating a sustained ‘perpetual cycle’ of appetite enhancement were observed. The GlyR protein may constitute a novel treatment target for the reduction of central orexigenic signals in obesity.
2044-4052
1-6
Manousopoulou, A.
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Koutmani, Y.
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Karaliota, S.
1fb6ee42-ddd4-4771-b8f8-c745bf47d60c
Woelk, C.H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Manolakos, E.S.
c6b4675f-0809-4b8e-9746-3b0e83d97b34
Karalis, K.
78203ad7-a96a-47c3-8ddf-efff265126b0
Garbis, S.D.
7067fd19-50c9-4d42-9611-f370289470bd
Manousopoulou, A.
9a5e4e75-cea9-4d0b-91c8-0fa2af02632f
Koutmani, Y.
d1c698f7-2233-4186-86c3-bcfbe5ec57dc
Karaliota, S.
1fb6ee42-ddd4-4771-b8f8-c745bf47d60c
Woelk, C.H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Manolakos, E.S.
c6b4675f-0809-4b8e-9746-3b0e83d97b34
Karalis, K.
78203ad7-a96a-47c3-8ddf-efff265126b0
Garbis, S.D.
7067fd19-50c9-4d42-9611-f370289470bd

Manousopoulou, A., Koutmani, Y., Karaliota, S., Woelk, C.H., Manolakos, E.S., Karalis, K. and Garbis, S.D. (2016) Hypothalamus proteomics from mouse models with obesity and anorexia reveals therapeutic targets of appetite regulation. Nutrition & Diabetes, 6 (e204), 1-6. (doi:10.1038/nutd.2016.10). (PMID:27110685)

Record type: Article

Abstract

Objective: This study examined the proteomic profile of the hypothalamus in mice exposed to a high-fat diet (HFD) or with the anorexia of acute illness. This comparison could provide insight on the effects of these two opposite states of energy balance on appetite regulation.

Methods: Four to six-week-old male C56BL/6J mice were fed a normal (control 1 group; n=7) or a HFD (HFD group; n=10) for 8 weeks. The control 2 (n=7) and lipopolysaccharide (LPS) groups (n=10) were fed a normal diet for 8 weeks before receiving an injection of saline and LPS, respectively. Hypothalamic regions were analysed using a quantitative proteomics method based on a combination of techniques including iTRAQ stable isotope labeling, orthogonal two-dimensional liquid chromatography hyphenated with nanospray ionization and high-resolution mass spectrometry. Key proteins were validated with quantitative PCR.

Results: Quantitative proteomics of the hypothalamous regions profiled a total of 9249 protein groups (q<0.05). Of these, 7718 protein groups were profiled with a minimum of two unique peptides for each. Hierachical clustering of the differentiated proteome revealed distinct proteomic signatures for the hypothalamus under the HFD and LPS nutritional conditions. Literature research with in silico bioinformatics interpretation of the differentiated proteome identified key biological relevant proteins and implicated pathways. Furthermore, the study identified potential pharmacologic targets. In the LPS groups, the anorexigen pro-opiomelanocortin was downregulated. In mice with obesity, nuclear factor-?B, glycine receptor subunit alpha-4 (GlyR) and neuropeptide Y levels were elevated, whereas serotonin receptor 1B levels decreased.

Conclusions: High-precision quantitative proteomics revealed that under acute systemic inflammation in the hypothalamus as a response to LPS, homeostatic mechanisms mediating loss of appetite take effect. Conversely, under chronic inflammation in the hypothalamus as a response to HFD, mechanisms mediating a sustained ‘perpetual cycle’ of appetite enhancement were observed. The GlyR protein may constitute a novel treatment target for the reduction of central orexigenic signals in obesity.

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Accepted/In Press date: 1 March 2016
e-pub ahead of print date: 25 April 2016
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 395367
URI: http://eprints.soton.ac.uk/id/eprint/395367
ISSN: 2044-4052
PURE UUID: 9df32e02-f6cd-468f-89ca-8749c53a666e
ORCID for S.D. Garbis: ORCID iD orcid.org/0000-0002-1050-0805

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Date deposited: 27 May 2016 11:25
Last modified: 15 Mar 2024 00:39

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Contributors

Author: A. Manousopoulou
Author: Y. Koutmani
Author: S. Karaliota
Author: C.H. Woelk
Author: E.S. Manolakos
Author: K. Karalis
Author: S.D. Garbis ORCID iD

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