Proteomic analysis of amniotic fluid in pregnancies with Down syndrome
Proteomic analysis of amniotic fluid in pregnancies with Down syndrome
Proteomic analysis is widely used for the detection of diagnostic markers. In the present study amniotic fluid supernatants?(AFS) from pregnancies with Down syndrome?(DS) fetuses and from chromosomally normal fetuses in the 17th?week of gestation were analyzed by 2-DE. Gel comparison revealed significant differences in the two groups. Spots with different expression levels were excised and proteins were identified by MALDI-MS and nano-ESI-MS/MS. Splicing factor arginine/serine-rich?4 (SFRS4; Q08170) was present only in AFS from DS fetuses and completely absent in the control group. Quantitative differences were detected for alpha-1-microglobulin (AMBP; P02760), collagen alpha?1?(I) chain (CO1A1; P02452), collagen alpha?1?(III) chain (CO3A1; P02461), collagen alpha?1?(V) chain?d (CO5A1; P20908), and basement membrane-specific heparin sulfate proteoglycan core protein (PGBM; P98160). These proteins were increased in cases with DS, whereas protein?IBP-1 (P08833) was decreased by 40% compared with chromosomally normal fetuses. Four proteins, CO1A1, CO3A1, CO5A1, and PGBM, appeared as fragments. As differentially expressed proteins were present in all pregnancies with DS tested, they may represent useful potential markers for prenatal diagnosis. However, for protein biomarkers to be of any clinical utility, systematic analysis of the maternal serum should be conducted.
4410-4419
Tsangaris, George Th.
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Karamessinis, Panagiotis
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Kolialexi, Aggeliki
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Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Antsaklis, Aris
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Mavrou, Ariadni
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Fountoulakis, Michael
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August 2006
Tsangaris, George Th.
9271b5ad-7a60-4e90-89f4-88ed0e1de520
Karamessinis, Panagiotis
e79360a4-cd88-46b2-9a52-a647e20ad74a
Kolialexi, Aggeliki
bac99d0b-b2e9-4062-95e6-632dff27d71d
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Antsaklis, Aris
86c86f67-e9e9-4eb5-8a29-2afde8e4e5d0
Mavrou, Ariadni
60eaf02e-06bf-4258-878d-b024f555a508
Fountoulakis, Michael
7918e434-8d49-49da-bd7e-45f71b6401b8
Tsangaris, George Th., Karamessinis, Panagiotis, Kolialexi, Aggeliki, Garbis, Spiros D., Antsaklis, Aris, Mavrou, Ariadni and Fountoulakis, Michael
(2006)
Proteomic analysis of amniotic fluid in pregnancies with Down syndrome.
Proteomics, 6 (15), .
(doi:10.1002/pmic.200600085).
(PMID:16847874)
Abstract
Proteomic analysis is widely used for the detection of diagnostic markers. In the present study amniotic fluid supernatants?(AFS) from pregnancies with Down syndrome?(DS) fetuses and from chromosomally normal fetuses in the 17th?week of gestation were analyzed by 2-DE. Gel comparison revealed significant differences in the two groups. Spots with different expression levels were excised and proteins were identified by MALDI-MS and nano-ESI-MS/MS. Splicing factor arginine/serine-rich?4 (SFRS4; Q08170) was present only in AFS from DS fetuses and completely absent in the control group. Quantitative differences were detected for alpha-1-microglobulin (AMBP; P02760), collagen alpha?1?(I) chain (CO1A1; P02452), collagen alpha?1?(III) chain (CO3A1; P02461), collagen alpha?1?(V) chain?d (CO5A1; P20908), and basement membrane-specific heparin sulfate proteoglycan core protein (PGBM; P98160). These proteins were increased in cases with DS, whereas protein?IBP-1 (P08833) was decreased by 40% compared with chromosomally normal fetuses. Four proteins, CO1A1, CO3A1, CO5A1, and PGBM, appeared as fragments. As differentially expressed proteins were present in all pregnancies with DS tested, they may represent useful potential markers for prenatal diagnosis. However, for protein biomarkers to be of any clinical utility, systematic analysis of the maternal serum should be conducted.
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Published date: August 2006
Organisations:
Cancer Sciences
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Local EPrints ID: 395428
URI: http://eprints.soton.ac.uk/id/eprint/395428
ISSN: 1615-9853
PURE UUID: 5757d8b2-99af-4382-9e5a-c5e5ddcd39b3
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Date deposited: 18 Jul 2016 11:02
Last modified: 15 Mar 2024 00:40
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Author:
George Th. Tsangaris
Author:
Panagiotis Karamessinis
Author:
Aggeliki Kolialexi
Author:
Spiros D. Garbis
Author:
Aris Antsaklis
Author:
Ariadni Mavrou
Author:
Michael Fountoulakis
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